ABSTRACT
Magnesium is presently attracting a lot of interest as a replacement to clinically used orthopaedic implant materials, due to its ability to solve the stress shielding problems, biodegradability and osteocompatibility. However, the strength of Mg is still lower than the requirement and it becomes worse after it starts degrading fast, while being exposed in living body environment. This research explores the effectiveness of 'grain refinement through deformation', as a tool to modify the strength (while keeping elastic modulus unaffected) of Mg based alloys in orthopaedic application. Hot rolled Mg-3wt% Zn alloy (MZ3) has been investigated for its potential in orthopaedic implant. Microstructure, mechanical properties, bio-corrosion properties and biocompatibility of the rolled samples are probed into. Grain size gets refined significantly with increasing amount of deformation. The alloy experiences a marked improvement in hardness, yield strength, ultimate tensile strength, strain and toughness with finer grain size. An increment in accelerated corrosion rate is noted with decreasing grain size, which is correlated to the increased grain boundary area and mechano-chemical dissolution. However, immersion test in simulated body fluid (SBF) reveals reduction in corrosion rate after third day of immersion. This was possible owing to precipitation of protective hydroxyapatite (HA) layer, formed out of the interaction of SBF and the alloy. More nucleation sites at the grain boundary for fine grained samples help in forming more HA and thus reduce the corrosion rate. Human osteosarcoma cells show less viability and adhesion on grain refined alloy.
Subject(s)
Absorbable Implants , Alloys , Magnesium , Orthopedics , Cell Line, Tumor , Corrosion , Humans , Materials Testing , Osteosarcoma/pathology , Tensile StrengthABSTRACT
The present work deals with development of ultrafine grained zircaloy-2 and studying its potential for orthopedic application. The multimodal structure, i.e. the combination of coarse, ultrafine grained (UFG) and nanograined structures of zircaloy-2 is obtained by cryorolling the bulk alloy followed by annealing at 400 °C, and 450 °C for 30 min. An estimation of surface wettability of the alloy was obtained through contact angle measurement. The bioactivity of the alloy samples was investigated by incubating bone marrow derived stem cells. The cellular attachment, adhesion and proliferation at different intervals of incubation were characterized by scanning fluorescent microscopy and MTT assay. Cell culture results indicated that liquid nitrogen rolled alloy samples exhibited excellent in-vitro biocompatibility together with satisfactory bioactivity. Excellent genomic expressions were observed for zircaloy 2 processed by cryorolling.