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1.
J Biol Chem ; 292(22): 9218-9228, 2017 06 02.
Article in English | MEDLINE | ID: mdl-28364043

ABSTRACT

Human leukocyte antigen (HLA)-DQ2.5 (DQA1*05/DQB1*02) is a class-II major histocompatibility complex protein associated with both type 1 diabetes and celiac disease. One unusual feature of DQ2.5 is its high class-II-associated invariant chain peptide (CLIP) content. Moreover, HLA-DQ2.5 preferentially binds the non-canonical CLIP2 over the canonical CLIP1. To better understand the structural basis of HLA-DQ2.5's unusual CLIP association characteristics, better insight into the HLA-DQ2.5·CLIP complex structures is required. To this end, we determined the X-ray crystal structure of the HLA-DQ2.5· CLIP1 and HLA-DQ2.5·CLIP2 complexes at 2.73 and 2.20 Å, respectively. We found that HLA-DQ2.5 has an unusually large P4 pocket and a positively charged peptide-binding groove that together promote preferential binding of CLIP2 over CLIP1. An α9-α22-α24-α31-ß86-ß90 hydrogen bond network located at the bottom of the peptide-binding groove, spanning from the P1 to P4 pockets, renders the residues in this region relatively immobile. This hydrogen bond network, along with a deletion mutation at α53, may lead to HLA-DM insensitivity in HLA-DQ2.5. A molecular dynamics simulation experiment reported here and recent biochemical studies by others support this hypothesis. The diminished HLA-DM sensitivity is the likely reason for the CLIP-rich phenotype of HLA-DQ2.5.


Subject(s)
HLA-DQ Antigens/chemistry , HLA-DQ alpha-Chains/chemistry , HLA-DQ beta-Chains/chemistry , Molecular Dynamics Simulation , Peptides/chemistry , Binding Sites , HLA-DQ Antigens/genetics , HLA-DQ alpha-Chains/genetics , HLA-DQ beta-Chains/genetics , Humans , Peptides/genetics
2.
Immunobiology ; 221(9): 964-74, 2016 09.
Article in English | MEDLINE | ID: mdl-27259371

ABSTRACT

Leptospirosis is a zoonotic disease and is caused by pathogenic species of the Leptospira genus, including Leptospira interrogans (L. interrogans). Humans, domestic and wild animals are susceptible to acute or chronic infection. The innate immune response is a critical defense mechanism against Leptospira interrogans, and has been investigated in mouse models. Murine Toll-like receptors (TLRs) have been shown to be key factors in sensing and responding to L. interrogans infection. Specifically, TLR2, TLR4 and the TLR adaptor molecule MyD88 are essential for host defense against L. interrogans; however, the role of the TLR adaptor molecule TIR-domain-containing adaptor-inducing interferon ß (TRIF) in the response to L. interrogans has not been previously determined. In the present study, TRIF was found to play an important role during leptospiral infection. Following challenge with L. interrogans, Trif(-/-) mice exhibited delayed weight gain compared to wild-type mice. Moreover, Trif(-/-) mice exhibited an increase in L. interrogans burden in the kidneys, lungs, and blood at early time points (less than 7days post infection). Multiple components of the innate immune responses were dampened in response to leptospiral infection including transcription and production of cytokines, and the humoral response, which suggested that TRIF contributes to expression and production of cytokines important for the host defense against L. interrogans.


Subject(s)
Adaptor Proteins, Vesicular Transport/immunology , Leptospirosis/immunology , Adaptor Proteins, Vesicular Transport/genetics , Animals , Cytokines/blood , Cytokines/genetics , Cytokines/immunology , Immunoglobulin A/immunology , Immunoglobulin M/immunology , Kidney/immunology , Leptospira/immunology , Lung/immunology , Mice, Inbred C57BL , Mice, Knockout , RNA, Messenger/metabolism
3.
J Virol ; 84(19): 10276-88, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20660183

ABSTRACT

The coronavirus nucleocapsid (N) protein plays an essential role in virion assembly via interactions with the large, positive-strand RNA viral genome and the carboxy-terminal endodomain of the membrane protein (M). To learn about the functions of N protein domains in the coronavirus mouse hepatitis virus (MHV), we replaced the MHV N gene with its counterpart from the closely related bovine coronavirus (BCoV). The resulting viral mutant was severely defective, even though individual domains of the N protein responsible for N-RNA, N-M, or N-N interactions were completely interchangeable between BCoV and MHV. The lesion in the BCoV N substitution mutant could be compensated for by reverting mutations in the central, serine- and arginine-rich (SR) domain of the N protein. Surprisingly, a second class of reverting mutations were mapped to the amino terminus of a replicase subunit, nonstructural protein 3 (nsp3). A similarly defective MHV N mutant bearing an insertion of the SR region from the severe acute respiratory syndrome coronavirus N protein was rescued by the same two classes of reverting mutations. Our genetic results were corroborated by the demonstration that the expressed amino-terminal segment of nsp3 bound selectively to N protein from infected cells, and this interaction was RNA independent. Moreover, we found a direct correlation between the N-nsp3 interaction and the ability of N protein to stimulate the infectivity of transfected MHV genomic RNA (gRNA). Our results suggest a role for this previously unknown N-nsp3 interaction in the localization of genomic RNA to the replicase complex at an early stage of infection.


Subject(s)
Coronavirus, Bovine/physiology , DNA-Directed RNA Polymerases/physiology , Murine hepatitis virus/physiology , Nucleocapsid Proteins/physiology , RNA, Viral/genetics , RNA-Dependent RNA Polymerase/physiology , Amino Acid Sequence , Animals , Cattle , Coronavirus Nucleocapsid Proteins , Coronavirus, Bovine/genetics , Coronavirus, Bovine/pathogenicity , DNA-Directed RNA Polymerases/genetics , Genome, Viral , Humans , Mice , Molecular Sequence Data , Murine hepatitis virus/genetics , Murine hepatitis virus/pathogenicity , Mutation , Nucleocapsid Proteins/chemistry , Nucleocapsid Proteins/genetics , Protein Structure, Tertiary , RNA-Dependent RNA Polymerase/genetics , Recombination, Genetic , Severe acute respiratory syndrome-related coronavirus/genetics , Severe acute respiratory syndrome-related coronavirus/pathogenicity , Severe acute respiratory syndrome-related coronavirus/physiology , Sequence Homology, Amino Acid , Transfection , Virulence/genetics , Virulence/physiology
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