Begomovirus-Host Interactions: Viral Proteins Orchestrating Intra and Intercellular Transport of Viral DNA While Suppressing Host Defense Mechanisms.

Begomoviruses, which belong to the Geminiviridae family, are intracellular parasites transmitted by whiteflies to dicotyledonous plants thatsignificantly damage agronomically relevant crops. These nucleus-replicating DNA viruses move intracellularly from the nucleus to the cytoplasm and then, like other plant viruses, cause disease by spreading systemically throughout the plant. The transport proteins of begomoviruses play a crucial role in recruiting host components for the movement of viral DNA within and between cells, while exhibiting functions that suppress the host's immune defense. Pioneering studies on species of the Begomovirus genus have identified specific viral transport proteins involved in intracellular transport, cell-to-cell movement, and systemic spread. Recent research has primarily focused on viral movement proteins and their interactions with the cellular host transport machinery, which has significantly expanded understanding on viral infection pathways. This review focuses on three components within this context: (i) the role of viral transport proteins, specifically movement proteins (MPs) and nuclear shuttle proteins (NSPs), (ii) their ability to recruit host factors for intra- and intercellular viral movement, and (iii) the suppression of antiviral immunity, with a particular emphasis on bipartite begomoviral movement proteins.

Biotechnological potential of microorganisms from textile effluent: isolation, enzymatic activity and dye discoloration.

Environmental pollution may be considered one of the main problems affecting the world population. As the effluents from textile industries are the largest representatives of sources of pollution of water bodies due to the disposal of colored compounds in the environment. Microorganisms capable of thriving in textile wastewater may exhibit metabolic machinery to synthesize a wide variety of enzymes and/or secondary metabolites of industrial interest. The present work investigated the biotechnological potential of filamentous fungi from wastewater of a textile industry for the production of laccase, cellulase, amylase and lipase enzymes and their potential for discoloration capacity of Remazol Brilliant Blue R synthetic dye. The isolate Aspergillus sydowii (ITF 30) presented the best cellulase (46.74 U mL-1), amylase, lipase and laccase (0.0273 U L-1) production, as well as RBBR dye discoloration ability in solid medium, followed by isolate Aspergillus sydowii (ITF 27) able to synthesize cellulase, amylase and laccase and had the capacity to discolor 74.7% of RBBR in liquid medium. The results of the present work encourage future studies of characterization, optimization and purification of the enzymes encountered, aiming to be used in bioremediation processes of textile industrial effluents.