Monitoring and assessment of radionuclide discharges from Temelín Nuclear Power Plant into the Vltava River (Czech Republic).

The paper summarizes impacts of the Temelín Nuclear Power Plant (NPP) on the Vltava and Labe River basins. The study is based on the results of long-term monitoring carried out before the plant operation (1989-2000), and subsequently during the plant operation (2001-2005). In the first period, the main objective was to determine background radionuclide levels remaining in the environment after global fallout and due to the Chernobyl accident. A decrease in the concentrations of (90)Sr, (134)Cs and (137)Cs, which was observed before the plant operation, continued also during the subsequent period. Apart from tritium, the results of the observation did not indicate any impacts of the plant on the concentrations of activation and fission products in the hydrosphere. The annual average tritium concentrations in the Vltava River were in agreement with predicted values. The maximum annual average tritium concentration (13.5 Bq L(-1)) was observed in 2004 downstream from the wastewater discharge in the Vltava River at Solenice. Estimated radiation doses for adults due to intakes of river water as drinking water contaminated by tritium are below 0.1 microSv y(-1).

The influence of temperature and hydration on the sorption properties of bentonite.

The effects of exposure to synthetic groundwater at elevated temperature gradients on the sorption properties of bentonite were investigated using the Mock-Up-Cz experiment. This experiment simulated the vertical placement of a container of radioactive waste according to the Swedish KBS-3 system for a period of more than 3 years. The mineralogical composition, as well as its chemical and physico-chemical properties, including the uptake of (99)TcO(4)(-) and (134)Cs(+), was used to evaluate the chemical changes caused by the long-term exposure of bentonite buffer to thermal and hydration gradients. It was found that the bentonite material was predominantly stable. No more than 2% of the montmorillonite was transformed due to thermal and moisture gradients. It was concluded that the new-formed mineral phases have no significant influence on the ion exchange and sorption properties of bulk bentonite samples.

Observed half-lives of 3H, 90Sr and 137Cs in hydrosphere in the Vltava River basin (Bohemia).

A near field study was set up to follow the effects of the Temelin nuclear power plant construction. Reference levels of artificial radionuclides were monitored in the Vltava River upper course and its tributaries in the period 1990-2001. Monitoring continued even after the waste water release startup during the pilot operation in 2002. The assessment of the (90)Sr and (137)Cs concentrations histories in ground water, river bottom sediments and fish showed a decreasing trend. This trend was not influenced by the nuclear power plant pilot operation. In the case of tritium, trend of increasing concentration had been already observed since the pilot operation startup. The monitoring of changes in concentrations of artificial and natural radionuclides in influenced and uninfluenced profiles will be maintained to assess the possible influence of the operation of the Temelin nuclear power plant.

Isotachophoretic determination of stability constants of Ho and Y complexes with diethylenetriaminepentaacetic acid and 1,4,7,10-tetraazadodecane-N,N',N'',N'''-tetraacetic acid.

The method of capillary isotachophoresis with conductivity detection was applied for the determination of the physico-chemical characteristics (conditional stability constants log beta') of holmium and yttrium complexes with DTPA (diethylenetriaminepentaacetic acid) and DOTA (1,4,7,10-tetraazadodecane-N,N',N'',N'''-tetraacetic acid). The log beta' determination is based on the linear relation between the stability constants of lanthanide-DTPA (lanthanide-DOTA) complexes and the reduction of the zone of the complex owing to the bleeding phenomena (liberating free metal ion). The stability constants calculated using this relationship are comparable with the literary data obtained by other methods for both holmium (log beta'(Ho-DTPA)=21.9, log beta'(Ho-DOTA)=24.5) and yttrium complexes (log beta'(Y-DTPA)=21.2, log beta'(Y-DOTA)=24.4). Capillary isotachophoresis was applied for the determination of the optimum composition of the reaction mixture (metal:ligand ratio) as well.

Capillary electrophoretic and thin-layer chromatographic characterization of rhenium complexation with 1-hydroxyethylidenediphosphonic acid.

1-Hydroxyethylidenediphosphonic acid (HEDP) labeled by short-lived radionuclides with the nuclear properties suitable for the therapeutical purposes (186Re, 188Re, 166Ho) is similar to the other phosphonates widely applied in the radiopharmaceutical field for the treatment of palliative bone metastases. One of the important steps for the preparation of compounds of radiopharmaceutical interest is the quality control comprehending the radiochemical and chemical purity determination. Chromatographic methods as TLC and HPLC are mostly used for this purpose. Our experiments were focused on the application of capillary electrophoresis with UV detection to the study of rhenium complexation with HEDP. The influence of pH, concentration of the ligand and the reaction time were determined. Taking in account our previous results, the Re:SnCl2 molar ratio 1:500 (for 0.1 mM Re) was applied to reduce perrhenate to lower oxidation states which enables the Re-HEDP complexation. Different background electrolytes were tested. The mixture of 40 mM Na2HPO4 with 15 mM HEDP adjusted to pH 8 was selected as the most suitable system because it enabled the separation of different forms of Re-HEDP complexes. The results obtained in this study were compared to those obtained by thin-layer chromatography with radiometric detection.

Study of perrhenate reduction by capillary electrophoresis.

The influence of perrhenate concentration, the concentration of the reducing agent and pH of the reaction mixture on the yield of perrhenate reduction were studied to find a possibility to decrease the stannous chloride concentration in the reaction mixture without negative changes on the yield of perrhenate reduction. Britton-Robinson buffer solutions were selected as the background electrolytes because of their buffering capacity in a wide pH interval. The highest degree of perrhenate reduction was obtained at pH 2 at perrhenate concentrations ranging from 10(-4) to 10(-3) mol/L. The stability of reduced rhenium against pH change from 2 to 5.5 and against dilution of rhenium in the reaction mixture to the concentration suitable for the application in radiotherapy were studied as well. The results obtained by capillary electrophoresis and by thin-layer chromatography with radiometric detection were compared.