Scanning electron microscopy (SEM) evaluation of sealing ability of MTA and EndoSequence as root-end filling materials with chitosan and carboxymethyl chitosan (CMC) as retrograde smear layer removing agents.

AIM: The purpose of this study was to evaluate the sealing ability of mineral trioxide aggregate (MTA) and EndoSequence with chitosan and carboxymethyl chitosan (CMC) as retrograde smear layer removing agents using scanning electron microscopy (SEM). MATERIALS AND METHODS: Forty human single rooted teeth were taken. Crowns were decoronated and canals were obturated. Apically roots were resected and retrograde cavities were done. Based on the type of retrograde material placed and the type of smear layer removal agent used for retrograde cavities, they were divided into four groups (N = 10): Group I chitosan with EndoSequence, group II chitosan with MTA, group III CMC with EndoSequence, and Group IV CMC with MTA. All the samples were longitudinally sectioned, and the SEM analysis was done for marginal adaptation. STATISTICAL ANALYSIS: Kruskal-Wallis and Mann-Witney analysis tests. RESULTS: SEM images showed the presence of less gaps in group III, i.e., CMC with EndoSequence when compared to other groups with statistically significant difference. CONCLUSION: Within the limited scope of this study, it was concluded that EndoSequence as retrograde material showed better marginal sealing ability.

"Evaluation of sealing ability of MM-MTA, Endosequence, and biodentine as furcation repair materials: UV spectrophotometric analysis".

AIM: To evaluate the sealing ability of MICRO-MEGA Mineral Trioxide Aggregate, Endosequence, Biodentine as furcation repair materials using a dye extraction leakage method. MATERIALS AND METHODS: Forty mandibular molars were randomly divided according to the material used for perforation repair. Group I- (left unsealed) control, Group II-MICRO-MEGA Mineral Trioxide Aggregate, Group III - Endosequence, Group IV - Biodentine. All samples were subjected to orthograde and retrograde methylene blue dye challenge followed by dye extraction with 65% nitric acid. Samples were then analyzed using Ultra violet (UV) Visible Spectrophotometer. RESULTS: Biodentine showed highest dye absorbance, whereas Endosequence showed lowest dye absorbance when compared with other repair materials. CONCLUSION: Within the limitations of this study, it was observed that Endosequence showed better sealing ability when compared with other root repair materials.