ABSTRACT
Cryptococcus albidus (Saito) Skinner, found frequently in the air of El-Minia, Egypt; in New Zealand and Budapest, and isolated occasionally from clinical specimens, has now been recognized as the cause of human diseases. Since isolation of this fungus from pigeon droppings has not previously been reported, this first paper reports results of its drug-susceptibility testing to gentamicin and 5-fluorocytosine, as well as its fungal cell antigenic cross reactivity with C. neoformans as detected by a Latex-Crypto-Antigen Detection System (LCAS).
Subject(s)
Columbidae/microbiology , Cryptococcus/isolation & purification , Feces/microbiology , Animals , Antigens, Fungal/immunology , Cross Reactions , Cryptococcus/drug effects , Cryptococcus/immunology , Cryptococcus neoformans/drug effects , Cryptococcus neoformans/immunology , Flucytosine/pharmacology , Gentamicins/pharmacology , Microbial Sensitivity Tests , TaiwanABSTRACT
This paper describes a scheme for differential identification of Candida species and other yeasts based on autoradiographic analysis of protein profiles of [35S]methionine-labeled cellular proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Using ATCC strains as references, protein profile analysis showed that different Candida and other yeast species produced distinctively different patterns. Good agreement in results obtained with this approach and with other conventional systems was observed. Being accurate and reproducible, this approach provides a basis for the development of an alternative method for the identification of yeasts isolated from clinical specimens.
Subject(s)
Candida/classification , Methionine/metabolism , Peptides/analysis , Yeasts/classification , Electrophoresis, Polyacrylamide Gel/methods , Molecular Weight , Radioisotope Dilution Technique , Species Specificity , Sulfur RadioisotopesABSTRACT
This is a study to modify and simplify the classical laboratory procedures of mycological diagnosis of Tinea unguium. By means of a tube KOH digestion technique for microscopic examination of nail clippings and a paper disc transfer technique using a more uniform wool-fiber-yeast extract culture fluid as inoculum for all tests necessary for fungal identification, dermatophytes can be identified to a species level. To authenticate the advantage of the etiologic diagnosis of Tinea unguium by mycological studies, the promising result of our previous clinical therapeutic trial with Griseofulvin was presented.
