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1.
Sci Rep ; 10(1): 9192, 2020 06 08.
Article in English | MEDLINE | ID: mdl-32513937

ABSTRACT

Nonhost resistance, a resistance of plant species against all nonadapted pathogens, is considered the most durable and efficient immune system in plants. To increase our understanding of the response of barley plants to infection by powdery mildew, Blumeria graminis f. sp. tritici, we used quantitative proteomic analysis (LC-MS/MS). We compared the response of two genotypes of barley cultivar Golden Promise, wild type (WT) and plants with overexpression of phytoglobin (previously hemoglobin) class 1 (HO), which has previously been shown to significantly weaken nonhost resistance. A total of 8804 proteins were identified and quantified, out of which the abundance of 1044 proteins changed significantly in at least one of the four comparisons ('i' stands for 'inoculated')- HO/WT and HOi/WTi (giving genotype differences), and WTi/WT and HOi/HO (giving treatment differences). Among these differentially abundant proteins (DAP) were proteins related to structural organization, disease/defense, metabolism, transporters, signal transduction and protein synthesis. We demonstrate that quantitative changes in the proteome can explain physiological changes observed during the infection process such as progression of the mildew infection in HO plants that was correlated with changes in proteins taking part in papillae formation and preinvasion resistance. Overexpression of phytoglobins led to modification in signal transduction prominently by dramatically reducing the number of kinases induced, but also in the turnover of other signaling molecules such as phytohormones, polyamines and Ca2+. Thus, quantitative proteomics broaden our understanding of the role NO and phytoglobins play in barley during nonhost resistance against powdery mildew.


Subject(s)
Ascomycota , Gene Expression Regulation, Plant/genetics , Gene Expression/genetics , Hemoglobins/genetics , Hemoglobins/metabolism , Hordeum/genetics , Hordeum/metabolism , Host Microbial Interactions/genetics , Proteome/genetics
2.
Acta Physiol (Oxf) ; 212(1): 106-18, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25041901

ABSTRACT

AIM: In essential hypertension (EH), the regulation of renal sodium excretion is aberrant. We hypothesized that in mild EH, (i) abnormal dynamics of plasma renin concentration (PRC) and atrial natriuretic peptide (ANP) are responsible for the exaggerated natriuresis, and (ii) exosomic protein patterns reflect the renal tubular abnormality involved in the dysregulation of sodium excretion. METHODS: After 2-week drug washout and 4-day diet, systemic and renal hemodynamics, cardio-renal hormones, glomerular filtration and renal excretion were studied in male patients during saline loading (SL). Excretion rates of exosome-related urinary proteins including apical membrane transporters were determined by proteomics-based methods. RESULTS: In patients, baseline renal vascular conductance was reduced (-44%, P < 0.001), but non-renal vascular conductances were normal while PRC was reduced and ANP elevated (both P < 0.01). SL induced exaggerated natriuresis and reduced PRC (P < 0.01), at normal suppression rate. SL increased arterial pressure in patients (+11 mmHg, P < 0.001), but not in controls; however, during time control, patients showed identical increases (+10 mmHg, P < 0.005) apparently dissociating arterial pressure from natriuresis. At baseline, excretion rates of 438 proteins ranged from 0.07 to 49.8 pmol (mmol creatinine)(-1); 12 proteins were found in all subjects, and 21 proteins were found in two or more patients, but not in controls. In patients, the excretion rate of retinoic acid-induced gene 2 protein was reduced, and excretion rates of other proteins showed increased variances compatible with pathophysiological and clinical applicability. CONCLUSION: Essential hypertension patients exhibit selective renal vasoconstriction and individually varying excretion rates of several exosome-related proteins. Hormonal changes, rather than arterial pressure, seem to cause exaggeration of natriuresis.


Subject(s)
Exosomes/metabolism , Hypertension/physiopathology , Kidney/blood supply , Membrane Proteins/urine , Natriuresis/physiology , Adult , Essential Hypertension , Humans , Hypertension/metabolism , Kidney/metabolism , Kidney/physiopathology , Kidney Function Tests , Male , Middle Aged , Proteomics , Vasoconstriction
3.
J Biomed Mater Res B Appl Biomater ; 89(2): 300-305, 2009 May.
Article in English | MEDLINE | ID: mdl-19343783

ABSTRACT

In this study, the growth factors in COLLOSSE were analyzed, using ELISA tests, mass spectrometry, western blotting, and a 24-day cell culture experiment using osteoblast-like cells. The results of the ELISA testing, mass spectrometry, and western blotting all confirmed that TGF-beta1 was the main growth factor in COLLOSSE at 55 ng/mg. The results from the culture test showed that the cell proliferation, alkaline phosphatase activity, and matrix calcification were all drastically changed by the addition of COLLOSSE, mirroring the effects of addition of TGF-beta1. We conclude that COLLOSSE is not only a rich source of TGFbeta-1, but also contains the growth factors TGFbeta-2, BMP-2, BMP-3, BMP-7, IGF-1, and possibly VEGF. Other growth factors might be present in COLLOSSE, but were not identified due to inherent detection limits of the used ELISA and mass spectrometry techniques. The number of osteoinductive factors in COLLOSSE causes a synergistic effect, explaining the new bone formation found in previously described in vivo studies, with much lower growth factor concentrations when compared with recombinant BMPs.


Subject(s)
Bone Morphogenetic Proteins/pharmacology , Collagen/pharmacology , Growth Substances/pharmacology , Guided Tissue Regeneration , Animals , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Bone Morphogenetic Proteins/chemistry , Cell Proliferation/drug effects , Cells, Cultured , Collagen/chemistry , Growth Substances/chemistry , Humans , Male , Materials Testing , Osteoblasts/cytology , Osteoblasts/physiology , Osteogenesis/drug effects , Osteogenesis/physiology , Rats , Rats, Wistar , Transforming Growth Factor beta1/pharmacology
4.
J Proteomics ; 71(1): 11-8, 2008 Apr 30.
Article in English | MEDLINE | ID: mdl-18541470

ABSTRACT

Plans for the European Proteomics Association (EuPA) were conceived and established during 2004 and 2005, and culminated in the formal inception of the organisation during the 4th HUPO World Congress held in Munich in 2005. The mission from the outset has been three-tiered and is to: i) strengthen the national Proteomics organizations in their efforts; ii) to co-ordinate and provide educational programs, and iii) to advance the networking of scientists through meetings, workshops and student exchange. Linked to the mission were objectives to emphasise the benefits and contributions of Proteomics to biological and industrial researchers, the general public and science policy makers in Europe. In addition, the EuPA set out to promote scientific exchange for all applications and technology development related to Proteomics, and coordinate joint activities of national Proteomics societies at the European level. To achieve these tasks an organisational structure was conceived whereby four Activity Committees (Conferences/Communications, Education, EuPA-HUPO-Interactions and Funding) were implemented and a General Council consisting of all member countries. The remarkable rise and progress the EuPA has achieved in this small time frame is reported here.


Subject(s)
Proteomics , Societies, Scientific/organization & administration , Europe , History, 21st Century , Proteomics/education , Proteomics/organization & administration , Societies, Scientific/history , Societies, Scientific/trends
5.
Rapid Commun Mass Spectrom ; 20(7): 1127-34, 2006.
Article in English | MEDLINE | ID: mdl-16521170

ABSTRACT

Polyacrylamide gel electrophoresis is widely used for protein separation and it is frequently the final step in protein purification in biochemistry and proteomics. Using a commercially available amine-reactive isobaric tagging reagent (iTRAQ) and mass spectrometry we obtained reproducible, quantitative data from peptides derived by tryptic in-gel digestion of proteins and phosphoproteins. The protocol combines optimized reaction conditions, miniaturized peptide handling techniques and tandem mass spectrometry to quantify low- to sub-picomole amounts of (phospho)proteins that were isolated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Immobilized metal affinity chromatography (FeIII-IMAC) was efficient for removal of excess reagents and for enrichment of derivatized phosphopeptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. Phosphopeptide abundance was determined by liquid chromatography/tandem mass (LC/MS/MS) using either MALDI time-of-flight/time-of-flight (TOF/TOF) MS/MS or electrospray ionization quadrupole time-of-flight (ESI-QTOF) MS/MS instruments. Chemically labeled isobaric phosphopeptides, differing only by the position of the phosphate group, were distinguished and characterized by LC/MS/MS based on their LC elution profile and distinct MS/MS spectra. We expect this quantitative mass spectrometry method to be suitable for systematic, comparative analysis of molecular variants of proteins isolated by gel electrophoresis.


Subject(s)
Amines/chemistry , Electrophoresis, Polyacrylamide Gel/methods , Microchemistry/methods , Peptide Mapping/methods , Phosphopeptides/analysis , Proteins/analysis , Spectrometry, Mass, Electrospray Ionization/methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Indicators and Reagents/chemistry , Isotope Labeling/methods , Reproducibility of Results , Sensitivity and Specificity
6.
Eur J Cancer ; 39(7): 989-95, 2003 May.
Article in English | MEDLINE | ID: mdl-12706369

ABSTRACT

Prostate cancer cells metastasise to bone causing a predominantly osteosclerotic response. It has previously been shown that PC3 cells secrete factors which stimulate the mitogenic activity of human bone marrow stromal (hBMS) cells. Some of these mitogens have been found to be proteins with a molecular weight between 20 and 30 kDa. Even though a number of investigations have been performed to identify the osteoblastic mitogenic factor or factors produced by prostate cancer cells, it is still unknown what causes the mitogenic activation of osteoblasts. Therefore, the aim of this study was to characterise the protein profile of conditioned medium (CM) from PC3 cells in the molecular weight range of 5-30 kDa using proteome analysis. A protein profile of the CM from PC3 cells was performed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Thirty protein spots with molecular weights ranging from 5 to 30 kDa were analysed by matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF MS). One of these spots was identified as cyclophilin A. We examined whether cyclophilin A alone or in combination with insulin-like growth factor-I (IGF-I) had any effects on the proliferation or differentiation of hBMS cells. Cyclophilin A at 1, 10, and 100 nM and cyclophilin A at 10 nM combined with 10 ng/ml IGF decreased the proliferation of hBMS cells up to 49+/-30, 38+/-29, 50+/-8 and 60+/-16%, respectively [mean (treated/control)+/-standard error of the means (SEM)] of control. IGF-I did not significantly affect these decreases. Cyclophilin A alone or in combination with IGF-I did not have any effect on differentiation (assessed by measuring the activity of alkaline phosphatase (ALP)). In conclusion, these results suggest cyclophilin A is not involved in the osteosclerotic effects seen when prostate cancer metastasises to bone.


Subject(s)
Bone Neoplasms/secondary , Cyclophilin A/metabolism , Osteoblasts/metabolism , Prostatic Neoplasms , Adult , Bone Neoplasms/pathology , Cell Division/drug effects , Cell Transformation, Neoplastic/drug effects , Cyclophilin A/pharmacology , Electrophoresis, Gel, Two-Dimensional/methods , Humans , Insulin-Like Growth Factor I/metabolism , Male , Middle Aged , Neoplasm Proteins/metabolism , Proteome
7.
Proteomics ; 1(2): 207-22, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11680868

ABSTRACT

Detailed characterization of phosphoproteins as well as other post-translationally modified proteins is required to fully understand protein function and regulatory events in cells and organisms. Here we present a mass spectrometry (MS) based experimental strategy for the identification and mapping of phosphorylation site(s) using only low-picomole amounts of phosphoprotein starting material. Miniaturized sample preparation methods for MS facilitated localization of phosphorylation sites in phosphoproteins isolated by polyacrylamide gel electrophoresis. Custom made, nanoscale immobilized Fe(III) affinity chromatography (Fe(III)-IMAC) columns were employed for enrichment of phosphorylated peptides from crude peptide mixtures prior to off-line analysis by matrix-assisted laser desorption/ionization (MALDI) MS or nanoelectrospray tandem mass spectrometry (MS/MS). An optimized and sensitive procedure for alkaline phosphatase treatment of peptide mixtures was implemented, which in combination with nano-scale Fe(III)-IMAC and MALDI-MS allowed unambiguous identification of phosphopeptides by observation of 80 Da mass shifts. Nanoelectrospray MS/MS was used for phosphopeptide sequencing for exact determination of phosphorylation sites. The advantages and limitations of the experimental strategy was demonstrated by enrichment, identification and sequencing of phosphopeptides from the model proteins ovalbumin and bovine beta-casein isolated by gel electrophoresis. Furthermore, an autophosphorylation site at Ser-3 in recombinant human casein kinase-2 beta subunit was determined. The potential of miniaturized Fe(III)-IMAC and MALDI-MS for characterization of in vivo phosphorylated proteins was demonstrated by identification of tryptic phosphopeptides derived from the human p47/phox phosphoprotein isolated by two-dimensional gel electrophoresis.


Subject(s)
Chromatography, Affinity/methods , Mass Spectrometry/methods , Phosphoproteins/isolation & purification , Amino Acid Sequence , Animals , Binding Sites , Casein Kinase II , Caseins/chemistry , Caseins/genetics , Cattle , Electrophoresis, Gel, Two-Dimensional , Humans , Iron , Molecular Sequence Data , NADPH Oxidases , Ovalbumin/chemistry , Ovalbumin/genetics , Peptide Fragments/chemistry , Peptide Fragments/genetics , Peptide Fragments/isolation & purification , Phosphoproteins/chemistry , Phosphoproteins/genetics , Protein Serine-Threonine Kinases/chemistry , Protein Serine-Threonine Kinases/genetics , Proteome , Spectrometry, Mass, Electrospray Ionization/methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Trypsin
8.
Proteomics ; 1(8): 955-66, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11683512

ABSTRACT

Identification and detailed characterization of complex mixtures of proteins separated by polyacrylamide gel electrophoresis (PAGE) require optimized and robust methods for interfacing electrophoretic techniques to mass spectrometry. Peptide mapping by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) is used as the first protein screening method in many laboratories because of its inherent simplicity, mass accuracy, sensitivity and relatively high sample throughput. We present a simplified sample preparation method for MALDI-MS that enables in-gel digestion of protein samples directly on the MALDI-MS metal probe. Removal of detergent and reagents as well as protein reduction and S-alkylation were performed prior to cutting of protein samples from the polyacrylamide gel slab. The general utility of this approach was demonstrated by on-probe digestion and MALDI-MS peptide mapping of femtomole amounts of standard proteins isolated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A representative set of 47 human proteins obtained from a silver stained two-dimensional electrophoretic gel was analyzed by the new method and resulted in a success rate for protein identification similar to that obtained by the traditional protocols for in-gel digestion and MALDI peptide mass mapping of human proteins, i.e. approximately 60%. The overall performance of the novel on-probe digestion method is comparable with that of the standard in-gel sample preparation protocol while being less labour intensive and more cost-effective due to minimal consumption of reagents, enzymes and consumables. Preliminary data obtained on a MALDI quadrupole-TOF tandem mass spectrometer demonstrated the utility of the on-probe digestion protocol for peptide mass mapping and peptide sequencing on this instrument. Automation of the on-probe protein digestion procedure and its combination with automated MALDI tandem mass spectrometry should be advantageous in proteomics research aimed at the systematic identification and analysis of large sets of proteins from electrophoretic gels.


Subject(s)
Proteins/chemistry , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Amino Acid Sequence , Electrophoresis, Gel, Two-Dimensional , Molecular Probes , Molecular Weight , Nanotechnology
9.
Protein Sci ; 10(10): 1989-2001, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11567090

ABSTRACT

Protein-nucleic acid complexes are commonly studied by photochemical cross-linking. UV-induced cross-linking of protein to nucleic acid may be followed by structural analysis of the conjugated protein to localize the cross-linked amino acids and thereby identify the nucleic acid binding site. Mass spectrometry is becoming increasingly popular for characterization of purified peptide-nucleic acid heteroconjugates derived from UV cross-linked protein-nucleic acid complexes. The efficiency of mass spectrometry-based methods is, however, hampered by the contrasting physico-chemical properties of nucleic acid and peptide entities present in such heteroconjugates. Sample preparation of the peptide-nucleic acid heteroconjugates is, therefore, a crucial step in any mass spectrometry-based analytical procedure. This study demonstrates the performance of four different MS-based strategies to characterize E. coli single-stranded DNA binding protein (SSB) that was UV-cross-linked to a 5-iodouracil containing DNA oligomer. Two methods were optimized to circumvent the need for standard liquid chromatography and gel electrophoresis, thereby dramatically increasing the overall sensitivity of the analysis. Enzymatic degradation of protein and oligonucleotide was combined with miniaturized sample preparation methods for enrichment and desalting of cross-linked peptide-nucleic acid heteroconjugates from complex mixtures prior to mass spectrometric analysis. Detailed characterization of the peptidic component of two different peptide-DNA heteroconjugates was accomplished by matrix-assisted laser desorption/ionization mass spectrometry and allowed assignment of tryptophan-54 and tryptophan-88 as candidate cross-linked residues. Sequencing of those peptide-DNA heteroconjugates by nanoelectrospray quadrupole time-of-flight tandem mass spectrometry identified tryptophan-54 and tryptophan-88 as the sites of cross-linking. Although the UV-cross-linking yield of the protein-DNA complex did not exceed 15%, less than 100 pmole of SSB protein was required for detailed structural analysis by mass spectrometry.


Subject(s)
DNA-Binding Proteins/chemistry , DNA/chemistry , Escherichia coli/chemistry , Tryptophan/chemistry , Amino Acids/chemistry , Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Cross-Linking Reagents , DNA/radiation effects , DNA-Binding Proteins/metabolism , Endopeptidase K/metabolism , Peptides/chemistry , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Ultraviolet Rays , Urea/chemistry
10.
Eur J Biochem ; 268(8): 2402-7, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11298759

ABSTRACT

HSP90 is one of the most abundant proteins in the cytosol of eukaryotic cells. HSP90 forms transient or stable complexes with several key proteins involved in signal transduction including protooncogenic protein kinases and nuclear receptors, it interacts with cellular structural elements such as actin-microfilament, tubulin-microtubule and intermediate filaments, and also exhibits conventional chaperone functions. This protein exists in two isoforms alpha-HSP90 and beta-HSP90, and it forms dimers which are crucial species for its biological activity. PAGE, ESI-MS and MALDI-MS were used to study HSP90 purified from pig brain. The two protein isoforms were clearly distinguished by ESI-MS, the alpha isoform being approximately six times more abundant than the beta isoform. ESI-MS in combination with lambda phosphatase treatment provided direct evidence of the existence of four phosphorylated forms of native pig brain alpha-HSP90, with the diphosphorylated form being the most abundant. For the beta isoform, the di-phosphorylated was also the most abundant. MALDI mass spectra of HSP90 samples after chemical cross-linking showed a high percentage of alpha-alpha homodimers. In addition, evidence for the existence of higher HSP90 oligomers was obtained.


Subject(s)
Brain/metabolism , HSP90 Heat-Shock Proteins/chemistry , HSP90 Heat-Shock Proteins/metabolism , Animals , Chromatography , Cross-Linking Reagents/pharmacology , Dimerization , Electrophoresis, Polyacrylamide Gel , Mass Spectrometry , Molecular Sequence Data , Phosphoric Monoester Hydrolases/pharmacology , Phosphorylation , Protein Isoforms , Protein Processing, Post-Translational , Spectrometry, Mass, Electrospray Ionization , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Swine
11.
J Cell Biol ; 152(3): 503-18, 2001 Feb 05.
Article in English | MEDLINE | ID: mdl-11157978

ABSTRACT

Proteins contained on purified COPII vesicles were analyzed by matrix-assisted laser desorption ionization mass spectrometry combined with database searching. We identified four known vesicle proteins (Erv14p, Bet1p, Emp24p, and Erv25p) and an additional nine species (Yip3p, Rer1p, Erp1p, Erp2p, Erv29p, Yif1p, Erv41p, Erv46p, and Emp47p) that had not been localized to ER vesicles. Using antibodies, we demonstrate that these proteins are selectively and efficiently packaged into COPII vesicles. Three of the newly identified vesicle proteins (Erv29p, Erv41p, and Erv46p) represent uncharacterized integral membrane proteins that are conserved across species. Erv41p and Erv46p were further characterized. These proteins colocalized to ER and Golgi membranes and exist in a detergent-soluble complex that was isolated by immunoprecipitation. Yeast strains lacking Erv41p and/or Erv46p are viable but display cold sensitivity. The expression levels of Erv41p and Erv46p are interdependent such that Erv46p was reduced in an erv41Delta strain, and Erv41p was not detected in an erv46Delta strain. When the erv41Delta or ev46Delta alleles were combined with other mutations in the early secretory pathway, altered growth phenotypes were observed in some of the double mutant strains. A cell-free assay that reproduces transport between the ER and Golgi indicates that deletion of the Erv41p-Erv46p complex influences the membrane fusion stage of transport.


Subject(s)
COP-Coated Vesicles/metabolism , Endoplasmic Reticulum/metabolism , Fungal Proteins/metabolism , Golgi Apparatus/metabolism , Intracellular Membranes/metabolism , Membrane Proteins/metabolism , Protein Transport/physiology , COP-Coated Vesicles/chemistry , Cell Fractionation , Databases, Factual , Endoplasmic Reticulum/chemistry , Fungal Proteins/genetics , Golgi Apparatus/chemistry , Immunoblotting , Membrane Proteins/genetics , Plasmids/genetics , Plasmids/metabolism , Precipitin Tests , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Yeasts/genetics , Yeasts/metabolism
12.
Rapid Commun Mass Spectrom ; 14(19): 1793-800, 2000.
Article in English | MEDLINE | ID: mdl-11006587

ABSTRACT

Analysis of phosphotyrosine and phosphoserine containing peptides by nano-electrospray Fourier transform ion cyclotron resonance (FTICR) mass spectrometry established electron capture dissociation (ECD) as a viable method for phosphopeptide sequencing. In general, ECD spectra of synthetic and native phosphopeptides appeared less complex than conventional collision activated dissociation (CAD) mass spectra of these species. ECD of multiply protonated phosphopeptide ions generated mainly c- and z(.)-type peptide fragment ion series. No loss of water, phosphate groups or phosphoric acid from intact phosphopeptide ions nor from the c and z(.) fragment ion products was observed in the ECD spectra. ECD enabled complete or near-complete amino acid sequencing of phosphopeptides for the assignment of up to four phosphorylation sites in peptides in the mass range 1400 to 3500 Da. Nano-scale Fe(III)-affinity chromatography combined with nano-electrospray FTMS/ECD facilitated phosphopeptide analysis and amino acid sequencing from crude proteolytic peptide mixtures.


Subject(s)
Phosphopeptides/chemistry , Amino Acid Sequence , Animals , Caseins/chemistry , Cattle , Chromatography, Affinity , Fourier Analysis , Mass Spectrometry , Molecular Sequence Data , Phosphorylation , Phosphoserine/chemistry , Phosphotyrosine/chemistry
13.
J Biol Chem ; 275(43): 33861-8, 2000 Oct 27.
Article in English | MEDLINE | ID: mdl-10906332

ABSTRACT

The low density lipoprotein (LDL) receptor is responsible for removing the majority of the LDL cholesterol from the plasma. Mutations in the LDL receptor gene cause the disease familial hypercholesterolemia (FH). Approximately 50% of the mutations in the LDL receptor gene in patients with FH lead to receptor proteins that are retained in the endoplasmic reticulum (ER). Misfolding of mutant LDL receptors is a probable cause of this ER retention, resulting in no functional LDL receptors at the cell surface. However, the specific factors and mechanisms responsible for retention of mutant LDL receptors are unknown. In the present study we show that the molecular chaperone Grp78/BiP co-immunoprecipitates with both the wild type and two different mutant (W556S and C646Y) LDL receptors in lysates obtained from human liver cells overexpressing wild type or mutant LDL receptors. A pulse-chase study shows that the interaction between the wild type LDL receptor and Grp78 is no longer detectable after 2(1/2) h, whereas it persists for more than 4 h with the mutant receptors. Furthermore, about five times more Grp78 is co-immunoprecipitated with the mutant receptors than with the wild type receptor suggesting that Grp78 is involved in retention of mutant LDL receptors in the ER. Overexpression of Grp78 causes no major alterations on the steady state level of active LDL receptors at the cell surface. However, overexpression of Grp78 decreases the processing rate of newly synthesized wild type LDL receptors. This indicates that the Grp78 interaction is a rate-limiting step in the maturation of the wild type LDL receptor and that Grp78 may be an important factor in the quality control of newly synthesized LDL receptors.


Subject(s)
Endoplasmic Reticulum/metabolism , HSP70 Heat-Shock Proteins/physiology , Membrane Proteins/physiology , Receptors, LDL/metabolism , 6-Aminonicotinamide/pharmacology , Amino Acid Sequence , Cells, Cultured , Endoplasmic Reticulum Chaperone BiP , Humans , Molecular Sequence Data , Molecular Weight , Mutation
14.
J Neurosci ; 20(11): 4069-80, 2000 Jun 01.
Article in English | MEDLINE | ID: mdl-10818142

ABSTRACT

Our understanding of the organization of postsynaptic signaling systems at excitatory synapses has been aided by the identification of proteins in the postsynaptic density (PSD) fraction, a subcellular fraction enriched in structures with the morphology of PSDs. In this study, we have completed the identification of most major proteins in the PSD fraction with the use of an analytical method based on mass spectrometry coupled with searching of the protein sequence databases. At least one protein in each of 26 prominent protein bands from the PSD fraction has now been identified. We found 7 proteins not previously known to be constituents of the PSD fraction and 24 that had previously been associated with the PSD by other methods. The newly identified proteins include the heavy chain of myosin-Va (dilute myosin), a motor protein thought to be involved in vesicle trafficking, and the mammalian homolog of the yeast septin protein cdc10, which is important for bud formation in yeast. Both myosin-Va and cdc10 are threefold to fivefold enriched in the PSD fraction over brain homogenates. Immunocytochemical localization of myosin-Va in cultured hippocampal neurons shows that it partially colocalizes with PSD-95 at synapses and is also diffusely localized in cell bodies, dendrites, and axons. Cdc10 has a punctate distribution in cell bodies and dendrites, with some of the puncta colocalizing with PSD-95. The results support a role for myosin-Va in transport of materials into spines and for septins in the formation or maintenance of spines.


Subject(s)
Myosin Heavy Chains , Myosin Type V , Nerve Tissue Proteins/analysis , Synapses/chemistry , Amino Acid Sequence , Animals , Cell Cycle Proteins/analysis , Cell Cycle Proteins/biosynthesis , Cell Cycle Proteins/genetics , Cells, Cultured , Cloning, Molecular , DNA, Complementary/biosynthesis , DNA, Complementary/chemistry , Dendrites/chemistry , Hippocampus/chemistry , Hippocampus/cytology , Hydrolysis , Immunoblotting , Immunohistochemistry , Intermediate Filament Proteins/analysis , Mass Spectrometry , Molecular Sequence Data , Neurons/chemistry , Peptides/analysis , Rats , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Trypsin
15.
Curr Genet ; 37(3): 200-8, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10794178

ABSTRACT

A differential protein display screen resulted in the identification of a 27-kDa protein which strongly accumulates during the senescence of Podospora anserina cultures grown under standard conditions. After partial determination of the amino-acid sequence by mass-spectrometry analysis of trypsin-generated fragments, pairs of degenerated primers were deduced and used to amplify parts of the sequence coding for the protein. These PCR products were utilized to select specific cDNA and genomic clones from DNA libraries of P. anserina. A subsequent DNA-sequence analysis revealed that the 27-kDa protein is encoded by a discontinuous gene, PaMth1, capable of coding for 240 amino acids. The first three amino-terminal residues appear to be removed post-translationally. The deduced amino-acid sequence shows significant homology to S-adenosylmethionine (SAM)-dependent methyltransferases. We hypothesize that the 27-kDa protein, PaMTH1, is involved in age-related methylation reactions protecting aging cultures against increasing oxidative stress.


Subject(s)
Ascomycota/genetics , Fungal Proteins/genetics , Methyltransferases/genetics , Amino Acid Sequence , Ascomycota/enzymology , Ascomycota/growth & development , Base Sequence , Cloning, Molecular , DNA, Fungal/chemistry , DNA, Fungal/genetics , Electrophoresis, Polyacrylamide Gel , Exons , Fungal Proteins/chemistry , Fungal Proteins/metabolism , Genes, Fungal/genetics , Introns , Molecular Sequence Data , Molecular Weight , Sequence Alignment , Sequence Analysis, DNA , Sequence Analysis, Protein , Sequence Homology, Amino Acid , Time Factors
16.
J Biol Chem ; 275(16): 11610-7, 2000 Apr 21.
Article in English | MEDLINE | ID: mdl-10766777

ABSTRACT

Dynamin I is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C (PKC). Dephosphorylation is required for synaptic vesicle retrieval, suggesting that its phosphorylation affects its subcellular localization. An in vitro phospholipid binding assay was established that prevents lipid vesiculation and dynamin lipid insertion into the lipid. Dynamin I bound the phospholipid in a concentration-dependent and saturable manner, with an apparent affinity of 230 +/- 51 nM. Optimal binding occurred with mixtures of phosphatidylserine and phosphatidylcholine of 1:3 with little binding to phosphatidylcholine or phosphatidylserine alone. Phospholipid binding was abolished after dynamin I phosphorylation by PKC and was restored after dephosphorylation by calcineurin. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry revealed the phosphorylation site in PKCalpha-phosphorylated dynamin I as a single site at Ser-795, located near a binding site for the SH3 domain of p85, the regulatory subunit of phosphatidylinositol 3-kinase. However, phosphorylation had no effect on dynamin binding to a bacterially expressed p85-SH3 domain. Thus, phosphorylation of dynamin I on Ser-795 prevents its association with phospholipid, providing a basis for the cytosolic localization of the minor pool of phospho-dynamin I that mediates synaptic vesicle retrieval in nerve terminals.


Subject(s)
GTP Phosphohydrolases/metabolism , Phospholipids/metabolism , Protein Kinase C/metabolism , Serine/metabolism , Adenine Nucleotides/metabolism , Amino Acid Sequence , Animals , Dynamin I , Dynamins , Electrophoresis, Polyacrylamide Gel , Escherichia coli , Guanine Nucleotides/metabolism , Molecular Sequence Data , Phosphorylation , Rabbits , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Structure-Activity Relationship , src Homology Domains
17.
Chem Res Toxicol ; 13(4): 237-44, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10775322

ABSTRACT

Disulfiram (DSF) is a drug used in aversion therapy to treat alcoholics and acts by inhibiting mitochondrial low-K(m) aldehyde dehydrogenase. Investigations into the mechanisms for in vivo inactivation suggest that the DSF metabolite S-methyl-N, N-diethylthiocarbamate sulfoxide reacts irreversibly with an active site Cys. This work aimed to determine if DSF generates monothiocarbamate adducts on cysteine residues in vivo by examining hemoglobin. Sprague-Dawley rats were treated with DSF po for 2, 4, and 6 weeks. Rats have four different globin beta-chains, of which three (beta-1-3) contain two cysteine residues each. MALDI-TOF MS analysis of two new globin species from DSF-treated rats collected by HPLC revealed increments of 99 Da above the mass of the unmodified chains (beta-2 and beta-3). In a separate experiment, the globin mixture was digested for 2 h with Glu-C and reanalyzed by MALDI-TOF MS. Results showed a peptide at m/z 2716.3 having a mass 99 Da higher than a known Cys-containing peptide. Subsequently, the Glu-C digest was analyzed using Q-TOF tandem MS, enabling observation of the +4 charge state of the peptide with m/z 2716.3. This peptide was fragmented to produce y-sequence ions that located the modification to Cys-125 (present on both beta-2 and beta-3). Cys-125 is the most reactive of two cysteine residues on these beta-chains. To confirm the structure of the modification, globin was hydrolyzed with 6 N HCl at 110 degrees C for 18 h. The adduct survived these conditions so that S-(N,N-diethylcarbamoyl)cysteine was detected in the hydrolysates of treated rats on the basis of comparison with the tandem MS spectrum of a standard. These results extend the findings of others obtained using glutathione conjugates and demonstrate the ability of DSF to covalently modify Cys residues of proteins in a manner consistent with the production of S-methyl-N, N-diethylthiocarbamate sulfoxide, or sulfone, intermediates.


Subject(s)
Alcohol Deterrents/metabolism , Disulfiram/metabolism , Hemoglobins/metabolism , Animals , Chromatography, High Pressure Liquid , Male , Mass Spectrometry , Rats , Rats, Sprague-Dawley
18.
Ugeskr Laeger ; 162(5): 643-8, 2000 Jan 31.
Article in Danish | MEDLINE | ID: mdl-10707596

ABSTRACT

Studies from western countries have shown that coeliac disease (CD) is common with prevalence figures about 1:300. The clinical spectrum varies greatly, steatorrhoea and weight loss affecting less than half of the patients. CD should be suspected in case of positive gliadin (IgA and IgG) and endomysial (IgA) antibodies. The diagnosis is based upon histological examination of duodenal biopsies taken during upper gastrointestinal endoscopy. Most patients respond quickly and satisfactorily to treatment with a gluten-free diet. This treatment also eliminates the excess risk of small bowel malignancy. Screening among first degree relatives and patients with insulin-dependent diabetes mellitus should be considered.


Subject(s)
Celiac Disease , Celiac Disease/complications , Celiac Disease/diagnosis , Celiac Disease/diet therapy , Diagnosis, Differential , Gastrointestinal Neoplasms/etiology , Humans , Risk Factors
19.
Ugeskr Laeger ; 162(5): 670-1, 2000 Jan 31.
Article in Danish | MEDLINE | ID: mdl-10707602

ABSTRACT

Coeliac disease in adults is an overlooked and undiagnosed illness. We present a case where a woman was diagnosed at the age of 59 despite symptoms suggestive of coeliac disease since childhood. At the time of diagnosis the patient had severe osteomalacia with multiple vertebral fractures of the thoracic and lumbar spine.


Subject(s)
Celiac Disease/diagnosis , Osteomalacia/etiology , Celiac Disease/complications , Diagnosis, Differential , Female , Humans , Middle Aged , Muscular Atrophy/diagnosis , Muscular Atrophy/etiology , Osteomalacia/diagnosis , Spinal Fractures/diagnosis , Spinal Fractures/etiology
20.
Nat Struct Biol ; 7(2): 122-6, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10655614

ABSTRACT

Rho family-specific guanine nucleotide dissociation inhibitors (RhoGDIs) decrease the rate of nucleotide dissociation and release Rho proteins such as RhoA, Rac and Cdc42 from membranes, forming tight complexes that shuttle between cytosol and membrane compartments. We have solved the crystal structure of a complex between the RhoGDI homolog LyGDI and GDP-bound Rac2, which are abundant in leukocytes, representing the cytosolic, resting pool of Rho species to be activated by extracellular signals. The N-terminal domain of LyGDI (LyN), which has been reported to be flexible in isolated RhoGDIs, becomes ordered upon complex formation and contributes more than 60% to the interface area. The structure is consistent with the C-terminus of Rac2 binding to a hydrophobic cavity previously proposed as isoprenyl binding site. An inner segment of LyN forms a helical hairpin that contacts mainly the switch regions of Rac2. The architecture of the complex interface suggests a mechanism for the inhibition of guanine nucleotide dissociation that is based on the stabilization of the magnesium (Mg2+) ion in the nucleotide binding pocket.


Subject(s)
Guanine Nucleotide Dissociation Inhibitors/chemistry , Guanine Nucleotide Dissociation Inhibitors/metabolism , rac GTP-Binding Proteins/chemistry , rac GTP-Binding Proteins/metabolism , Amino Acid Sequence , Binding Sites , Cell Membrane/metabolism , Crystallography, X-Ray , Guanosine Diphosphate/chemistry , Guanosine Diphosphate/metabolism , Guanosine Triphosphate/metabolism , Hydrolysis , Lipid Metabolism , Models, Molecular , Molecular Sequence Data , Protein Conformation , Proteins/chemistry , Proteins/metabolism , rho GTP-Binding Proteins/chemistry , rho GTP-Binding Proteins/metabolism , rho-Specific Guanine Nucleotide Dissociation Inhibitors , RAC2 GTP-Binding Protein
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