1.
J Am Chem Soc
; 130(13): 4246-7, 2008 Apr 02.
Article
in English
| MEDLINE
| ID: mdl-18331041
ABSTRACT
We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mum in live tissues using two-photon microscopy.
Subject(s)
Fluorescent Dyes/chemistry , Membrane Lipids/chemistry , Membrane Microdomains/chemistry , Naphthalenes/chemistry , Animals , Cells, Cultured , Fluorescence , Fluorescent Dyes/pharmacokinetics , Hippocampus/cytology , Hippocampus/drug effects , Hippocampus/metabolism , Macrophages/drug effects , Macrophages/metabolism , Membrane Microdomains/drug effects , Membrane Microdomains/metabolism , Mice , Molecular Structure , Organ Culture Techniques , Photons , Pyramidal Cells/drug effects , Pyramidal Cells/metabolism , Rats
2.
Angew Chem Int Ed Engl
; 47(12): 2231-4, 2008.
Article
in English
| MEDLINE
| ID: mdl-18273843
