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Prep Biochem Biotechnol ; 35(3): 257-61, 2005.
Article in English | MEDLINE | ID: mdl-16109637

ABSTRACT

We describe a rapid and simple procedure for homogenizing leaf samples suitable for mini/midi-scale DNA preparation in rice. The methods used tungsten carbide beads and general vortexer for homogenizing leaf samples. In general, two samples can be ground completely within 11.3+/-1.5 sec at one time. Up to 20 samples can be ground at a time using a vortexer attachment. The yields of the DNA ranged from 2.2 to 7.6 microg from 25-150 mg of young fresh leaf tissue. The quality and quantity of DNA was compatible for most of PCR work and RFLP analysis.


Subject(s)
DNA, Plant/isolation & purification , Oryza/chemistry , Methods , Oryza/genetics , Plant Leaves/chemistry , Plant Leaves/genetics , Tungsten Compounds/chemistry
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