ABSTRACT
Mature biofilms are highly resistant to antimicrobial agents due to the presence of extracellular polymeric substances (EPS), which inhibit the penetration of external molecules. In this study, we developed a coordination compound consisting of zinc chloride and erythritol that exhibits penetrating and bactericidal activity against Streptococcus mutans biofilms. An in vitro biofilm model was established in microplates, and bactericidal activity against biofilms was evaluated using an Alamar blue assay. The cause of the antimicrobial activity of the zinc-erythritol mixture on mature biofilms was demonstrated using fast atom bombardment-mass spectrometry, confocal laser scanning microscopy and atomic force microscopy. We demonstrated that zinc chloride spontaneously formed cationic complexes with erythritol in water. The zinc-erythritol complexes reduced intra- and inter-molecular interactions between bacterial exopolysaccharides, a major component of EPS. This activity was confirmed by measuring the attenuation of the hardness of dried polysaccharides isolated from S. mutans biofilms. The reduction in the interactions between polysaccharides allowed the complexes to penetrate into biofilms and kill the embedded bacteria. While approximately 13% of biofilm-associated microbes were killed by a 10 min treatment with 6.6 mM zinc chloride, 45% were killed when a solution containing 19.8 mM erythritol and 6.6 mM zinc chloride was used. This strategy of leveraging the coordination properties of metal ions with sugar alcohols provides a simple way to effectively remove mature biofilms using only conventional substances without the need for intricate chemical synthesis processes.
Subject(s)
Anti-Infective Agents/pharmacology , Biofilms/drug effects , Coordination Complexes/pharmacology , Streptococcus mutans/drug effects , Anti-Infective Agents/chemistry , Coordination Complexes/chemistry , Humans , Microscopy, Confocal , Polysaccharides/chemistry , Polysaccharides/pharmacology , Streptococcus mutans/pathogenicity , Sugar Alcohols/chemistry , Sugar Alcohols/pharmacology , Zinc/chemistry , Zinc/pharmacologyABSTRACT
Efficient nanofocusing of light into a gap plasmon waveguide using three-dimensional mode conversion in a strip plasmonic directional coupler is proposed. Unlike conventional nanofocusing using tapering structures, a plasmonic directional coupler converts E(z)-type odd mode energy into E(y)-type gap plasmon mode by controlling phase mismatch and gap spacing. The simulation result shows the maximum electric field intensity increases up to 58.1 times the input intensity, and 17.3% of the light is focused on the nano gap region.
