A strategy to reduce the use of fish in acute ecotoxicity testing of new chemical substances notified in the European Union.

This study explores the applicability of a fish acute threshold (step-down) test approach for the assessment of new chemical substances notified in the EU. The proposed approach basically implies replacing the fish LC50 toxicity test with a simple acute threshold test and thus reducing the number of fish used and also costs. The fish test would be performed only at one concentration, the lowest between the EC50 concentrations obtained with previous testing with algae and daphnia. When fish would be more sensitive than algae and daphnia, testing with fish would be continued at lower concentrations (step-down). From step-down test results the LC50 value can be obtained by applying the binominal method of interpolation. These data can be used together with algal and daphnid data to provide the same Predicted No Effect Concentration values. The acute aquatic toxicity data used in this evaluation were extracted from the New Chemicals Database of the European Chemicals Bureau. The results show that 53.6-71.2% reduction of the number of fish used would be possible when applying this new testing strategy and suggest its use for regulatory purposes.

Qsar investigation of a large data set for fish, algae and Daphnia toxicity.

A large data set containing values for fish, algae and Daphnia toxicity for more than 2000 chemicals and mixtures was investigated. The data set was taken from the New Chemicals Data Base of the European Union [hosted by the European Chemicals Bureau, Joint Research Centre, European Commission (http://ecb.jrc.it)]. The data are submitted by industry, according to the requirements of EU Council Directive 67/548/EEC as amended for the seventh time by EU Council Directive 92/32/EEC. The toxicities of neutral chemicals, salts, metal complexes, as well as chemical mixtures were extracted. A baseline effect was demonstrated by chemicals known to act by a narcotic mechanism of action, i.e., a relationship was observed between the toxicity and the logarithm of the octanol-water partition coefficient (log P). However, the prediction of the toxicity of more reactive chemicals was found to require the use of additional descriptors.

Johnston's organ and central organ in Nezara viridula (L.) (Heteroptera, Pentatomidae).

The fine structure of Johnston's organ and central organ in Nezara viridula (Heteroptera, Pentatomidae) is described. Johnston's organ consists of 45 scolopidia distributed around the periphery of the distal part of the third antennal segment (distal pedicellite). The scolopidia are anchored separately in invaginations of joint cuticle between the pedicel and flagellum. The scolopidia are amphinematic and each scolopidium comprises three sensory cells and three enveloping cells. The latter are a proximal scolopale cell with a typical labyrinth, an attachment cell filled with many microtubules, and a distal accessory cell also filled with microtubules. Axons of 17 scolopidia gather and join one antennal nerve; 28 scolopidia of the opposite side, extend axons into the other antennal nerve. The central organ consists of seven mononematic scolopidia, which comprise of one or two sensory cells. They anchor in the same joint as the scolopidia of Johnston's organ. The sensory cell bodies of the central organ are located close to the antennal nerves, more proximally than those of Johnston's organ. The axons of four scolopidia join one antennal nerve and those of the remaining three scolopidia join the other antennal nerve. Enveloping cells similar to those in Johnston's organ are present in the central organ.

Mechanoreceptors in insects: Johnston's organ in Nezara viridula (L) (Pentatomidae, Heteroptera).

Behavioural observations of Nezara viridula suggested that the antennae could be involved in detecting the substrate vibrations important in intraspecific communication of these insects. Therefore the vibrosensitive properties of Johnston's organ, a mechanoreceptor sensitive to the movements of the antennal flagellum relative to pedicel, were investigated. Vibrational stimuli were applied to the proximal flagellar segment where activity in the antennal nerve was recorded via a tapered tungsten electrode inserted into the second antennal segment where Johnston's organ is located. Sensory cells of Johnston's organ scolopidia respond to low frequency substrate vibrations (below 200 Hz). Both fast and slowly adapting receptor cells are present. However, the sensitivity of Johnston's organ to substrate vibrations in Nezara viridula is much lower than that of vibroreceptors in their legs which are known to be involved in intraspecific vibrational communication.

Structure of atympanate tibial organs in legs of the cave-living ensifera, Troglophilus neglectus (Gryllacridoidea, Raphidophoridae).

Troglophilus neglectus (Gryllacridoidea, Raphidophoridae) is a nocturnal Ensifera which can be found in caves of Slovenia. The anatomy of the tibial organs in the fore-, mid-, and hindlegs, as well as the external morphology of the proximal fore-tibia and the prothoracic tracheal system, is described comparatively. In the prothorax and in the forelegs, no sound-conducting structures such as an acoustic trachea, enlarged spiracles, or tympana are developed. A group of 8-10 campaniform sensillae is located in the dorsal cuticle of the proximal tibia. In each leg, the tibial organ complex is built up by two scolopale organs, the subgenual organ and the intermediate organ; the structure and the number of scolopidia is similar in each leg. No structure resembling the crista acoustica is found. The subgenual organ contains around 30 scolopidia; the intermediate organ is subdivided into a proximal part containing 8-9 scolopidia and a distal part with 5-6 scolopidia. The two groups of scolopidia are not directly connected to the tracheal system. The tibial organs in the forelegs are insensitive to airborne sound, and they appear to be more primitive compared to those found in members of the Tettigoniidae and the Gwllidae. The results indicate that the complex tibial organs in all legs of T. neglectus are primarily vibrosensitive. © 1995 Wiley-Liss, Inc.