[Oral mucosa analog allografts in non-consanguineous rats].

INTRODUCTION: Although there are therapeutic options for the treatment of oral mucosa defects, the need for functional, anatomical and aesthetically similar substitutes persists, as well as for solutions to reduce autologous grafts morbidity. OBJECTIVE: To determine clinical and histological compatibility of equivalent oral mucosa allografts generated through tissue engineering in non-consanguineous rats. MATERIALS AND METHODS: We used a sample of oral mucosa from Sprague Dawley rats to obtain a fibroblast culture and a keratinocytes and fibroblasts co-culture. In both cases, we used a commercial collagen membrane as "scaffold". After ten weeks of culture, we grafted the resulting membranes into four Wistar rats. The first phase of the study was the development of the oral mucosa equivalents generated by tissue engineering. Then, we implanted them in immunocompetent Wistar rats, and finallywe evaluated the clinical and histological features of the allografts. RESULTS: In vivo evaluation of mucosal substitutes showed a correct integration of artificial oral mucosa in immunocompetent hosts, with an increase in periodontal biotype and the creation of a zone with increased keratinization. Histologically, the tissue was similar to the control oral mucosa sample with no inflammatory reaction nor clinical or histological rejection signs. CONCLUSION: The equivalent oral mucosa allografts generated by tissue engineering showed clinical and histological compatibility.

Comportamiento clínico de análogo de mucosa bucal injertado en recesiones gingivales inducidas en modelos animales / Clinical behavior of oral mucosa analoguegrafted in gingival recession inducedin animal models

Objetivo: determinar la actividad de un injerto basado en el cocultivo de fibroblastos gingivales y queratinocitos en membrana de colágeno comercial Mem-Lok(R) (BioHorizons), Alabama, Estados Unidos) en el tratamiento de recesiones gingivales. Materiales y métodos. Esta investigación fue descriptiva y de diseño experimental. La muestra se conformó de 10 ratas Sprague Dawley a las que se indujeron recesiones gingivales. A 8 de ellas se les aplicó el injerto y las 2 restantes no recibieron tratamiento. Resultados: el análisis descriptivo de los resultados determinó la posibilidad de obtener un cocultivo celular. Luego de la aplicación del injerto, las características clínicas periodontales indicaron salud, consistencia firme, textura a manera de puntillado, contorno festoneado, biotipo grueso, sondaje periodontal de 1 mm y posición de la encía a nivel del límite amelocementario. Conclusiones: el injerto aplicado logró una cobertura radicular del 100 por ciento en todos los casos. No se observó sangrado ni contracción cicatrizal.

Aim: to determine the effectiveness of a graft based onco-cultivation of gingival fibroblast and keratinocytes in commercial collagen membrane Mem-Lok® (BioHorizons, Alabama,USA) in the treatment of gingival recessions. Materials and methods: This research was descriptiveand experimental in design. The sample was composed of 10 Sprague Dawley rats which were induced gingival recession; two of them were not treated and the graft was applied in eightof them. Results: A descriptive analysis of the results was performed, which showed that it was possible to obtain a cellco-culture. After the application of the graft, clinical periodontal characteristics were observed that indicated health: the consistency was firm, the texture resembled dots, scallopedand knife edge margin, a thick biotype and the depth ofgingival sulcus was 1 mm. Conclusions: The applied graft achieved a 100% radicularcoverage in all cases and no bleeding or scar contractionwas observed.