ABSTRACT
Herein, a simple, sensitive and selective turn-on fluorescent method for the determination of epinephrine (EP), an important hormone and neurotransmitter, is described. The method is based on the interaction of EP with PEG6000 coated carbon nanoparticles (PCN) resulting in the fluorescence enhancement of PCN. A linear relationship was obtained between the fluorescence intensity and concentration of EP in the 9.90 × 10(-9) - 1.07 × 10(-7) M range; the detection limit being 7.59 × 10(-10) M. Furthermore, we demonstrated the application of the present approach in a synthetic urine sample, which suggested its great potential for diagnostic purposes.
Subject(s)
Epinephrine/urine , Fluorometry/methods , Green Chemistry Technology/methods , Urinalysis/methods , Humans , Limit of DetectionABSTRACT
Although several methods have been reported for the determination of nitrite, development of simple, rapid,cost-effective, and sensitive sensors still remains a great challenge.Here, a simple yet very sensitive fluorescent method for the rapid determination of nitrite has been developed. The fluorescent assay is based on the aggregation of PEG6000 coated carbon nanoparticles (PCNs) in the presence of NO2(-) ions resulting in fluorescence enhancement. The assay response was linear in a concentration range of 3.84 × 10(-8) M to 4.97 × 10(-9) M with a detection limit of 2.32 × 10(-9) M. The proposed sensor exhibited good selectivity towards NO2(-) and was successfully applied for the determination of nitrite in milk, borewell water and soil samples.
