Distribution of Inflammatory Infiltrate in Feline Mammary Lesions: Relationship With Clinicopathological Features.

Inflammation is a frequent finding in feline mammary neoplasms. Recent research suggests that the presence and location of tumour-associated immune cells might play a significant role in the clinical outcome of feline mammary carcinomas. The present study aimed to characterise the overall inflammatory infiltrates in healthy, hyperplastic/dysplastic, benign and malignant lesions of the feline mammary gland, and to evaluate its association with clinicopathological features. Perilesional and intralesional inflammatory foci were evaluated in 307 lesions from 185 queens, and categorised according to its distribution and intensity. The presence, location and density of tertiary lymphoid structures were also assessed. A control group included 24 queens without mammary changes. The presence of intralesional and perilesional inflammatory infiltrate was observed in a majority of the lesions (80.8% and 90.2%, respectively), but differed according to the type of mammary lesion, being more remarkable in malignant neoplasms. Only scarce individual cells were observed in 28.1% of the normal mammary glands. Data analysis revealed statistically significant associations (p < 0.05) between the presence of a more prominent intralesional and perilesional inflammatory reaction and several clinicopathological features associated with worse prognosis, including clinical stage, tumour size, mitotic count, lymphovascular invasion and lymph node metastasis. Furthermore, tertiary lymphoid structures were significantly more frequent in tumours with an infiltrative growth and lymph node metastasis. According to our results, the inflammatory reaction present in different types of feline mammary lesions is associated with the development of more aggressive tumours.

What If Root Nodules Are a Guesthouse for a Microbiome? The Case Study of Acacia longifolia.

Acacia longifolia is one of the most aggressive invaders worldwide whose invasion is potentiated after a fire, a common perturbation in Mediterranean climates. As a legume, this species establishes symbioses with nitrogen-fixing bacteria inside root nodules; however, the overall microbial diversity is still unclear. In this study, we addressed root nodules' structure and biodiversity through histology and Next-Generation Sequencing, targeting 16S and 25S-28S rDNA genes for bacteria and fungi, respectively. We wanted to evaluate the effect of fire in root nodules from 1-year-old saplings, by comparing unburnt and burnt sites. We found that although having the same general structure, after a fire event, nodules had a higher number of infected cells and greater starch accumulation. Starch accumulated in uninfected cells can be a possible carbon source for the microbiota. Regarding diversity, Bradyrhizobium was dominant in both sites (ca. 77%), suggesting it is the preferential partner, followed by Tardiphaga (ca. 9%), a non-rhizobial Alphaproteobacteria, and Synechococcus, a cyanobacteria (ca. 5%). However, at the burnt site, additional N-fixing bacteria were included in the top 10 genera, highlighting the importance of this process. Major differences were found in the mycobiome, which was diverse in both sites and included genera mostly described as plant endophytes. Coniochaeta was dominant in nodules from the burnt site (69%), suggesting its role as a facilitator of symbiotic associations. We highlight the presence of a large bacterial and fungal community in nodules, suggesting nodulation is not restricted to nitrogen fixation. Thus, this microbiome can be involved in facilitating A. longifolia invasive success.

From a Lose-Lose to a Win-Win Situation: User-Friendly Biomass Models for Acacia longifolia to Aid Research, Management and Valorisation.

Woody invasive species pose a big threat to ecosystems worldwide. Among them, Acacia longifolia is especially aggressive, fundamentally changing ecosystem structure through massive biomass input. This biomass is rarely harvested for usage; thus, these plants constitute a nuisance for stakeholders who invest time and money for control without monetary return. Simultaneously, there is an increased effort to valorise its biomass, e.g., for compost, growth substrate or as biofuel. However, to incentivise A. longifolia harvest and usage, stakeholders need to be able to estimate what can be obtained from management actions. Thus, the total biomass and its quality (C/N ratio) need to be predicted to perform cost-benefit analyses for usage and determine the level of invasion that has already occurred. Here, we report allometric biomass models for major biomass pools, as well as give an overview of biomass quality. Subsequently, we derive a simplified volume-based model (BM ~ 6.297 + 0.982 × Vol; BM = total dry biomass and Vol = plant volume), which can be applied to remote sensing data or with in situ manual measurements. This toolkit will help local stakeholders, forest managers or municipalities to predict the impact and valorisation potential of this invasive species and could ultimately encourage its management.

Dietary Advice in Hemodialysis Patients: Impact of a Telehealth Approach During the COVID-19 Pandemic.

OBJECTIVE: The purpose of this study was to assess the effect of a telehealth-delivered nutritional intervention via telephone in maintenance hemodialysis (HD) patients during the coronavirus outbreak. METHODS: This was a multicenter, observational, prospective, and longitudinal study of 156 patients undergoing maintenance HD from 15 dialysis units conducted during the COVID-19 pandemic. We assigned patients to receive dietary counseling through a phone call, according to their biochemical and nutritional parameters. Dry weight, intradialytic weight gain percentage (%IDWG), body mass index, potassium, phosphorus, calcium, calcium/phosphorus product, normalized protein catabolic rate, albumin, and hemoglobin were recorded at baseline and 1 month after nutrition counseling. RESULTS: The prevalence of hyperkalemia and hyperphosphatemia decreased significantly after dietary advice. A statistically significant reduction in serum potassium and phosphorus levels was observed in patients receiving counseling for hyperkalemia and hyperphosphatemia. In addition, there was a statistically significant decrease in the prevalence of hypophosphatemia. We also observed a significant decrease in %IDWG, although no statistically significant differences were detected in patients with high %IDWG. The data demonstrated statistically significant differences in potassium and phosphorus values when the person receiving the phone contact was the patient or the caregiver. The main statistically significant differences in hypophosphatemia %IDWG were only observed when contact was made directly with the patient. No differences were observed when the contact was made through nursing homes. CONCLUSION: Our results suggest that telehealth-delivered dietary interventions can improve the clinical and nutritional parameters of HD patients. Consequently, this strategy may be effective for promoting continuous nutritional monitoring in these patients, in particular when conducting a face-to-face option is not crucial.

Triathlon-related musculoskeletal injuries: a study on a Portuguese Triathlon Championship.

BACKGROUND: A triathlon consists of 3 modalities: swimming, cycling and running. Its higher training frequency, the environmental factors and extrinsic factors (technique and equipment) may lead to injuries. This study aimed to determine injury epidemiology in triathletes, as well as their type, location, mechanism of injury, and risk factors. METHODS: The sample was constituted of 174 triathletes who participated in the Triathlon Club Portuguese Championship, being 131 (75.3%) male, aged 18 to 70 years old (36.09±11.03). The measurement instrument was a questionnaire concerning the characterization of the population and aspects related to the modality and injuries. RESULTS: One hundred and twenty (69%) athletes reported one injury since the beginning of their practice, and 95 (54.6%) had an injury in the previous year, with a total of 130 injuries. There were 2.39 injuries per 1,000 hours of triathlon training. The most common injuries were muscle contusion (31.5%) and inflammatory injuries (19.2%), located in the knee (22.3%) and the leg (18.5%). Overtraining (43.1%) was the mechanism that led to the highest occurrence of injuries. Fourteen (10.8%) injuries occurred during swimming, 23 (17.7%) during cycling, and 93 (71.5%) during running. No statistical significance was observed between the risk factors analyzed and the occurrence of injuries. CONCLUSIONS: Triathlon practice is associated with a high prevalence of injuries, being contusions, knee, and overtraining the most common type, location, and mechanism of injury respectively. It is necessary to create injury prevention strategies, including specific training and suitable materials for use by athletes.

Triathlon-related musculoskeletal injuries: a study on a Portuguese Triathlon Championship

SUMMARY BACKGROUND: A triathlon consists of 3 modalities: swimming, cycling and running. Its higher training frequency, the environmental factors and extrinsic factors (technique and equipment) may lead to injuries. This study aimed to determine injury epidemiology in triathletes, as well as their type, location, mechanism of injury, and risk factors. METHODS: The sample was constituted of 174 triathletes who participated in the Triathlon Club Portuguese Championship, being 131 (75.3%) male, aged 18 to 70 years old (36.09±11.03). The measurement instrument was a questionnaire concerning the characterization of the population and aspects related to the modality and injuries. RESULTS: One hundred and twenty (69%) athletes reported one injury since the beginning of their practice, and 95 (54.6%) had an injury in the previous year, with a total of 130 injuries. There were 2.39 injuries per 1,000 hours of triathlon training. The most common injuries were muscle contusion (31.5%) and inflammatory injuries (19.2%), located in the knee (22.3%) and the leg (18.5%). Overtraining (43.1%) was the mechanism that led to the highest occurrence of injuries. Fourteen (10.8%) injuries occurred during swimming, 23 (17.7%) during cycling, and 93 (71.5%) during running. No statistical significance was observed between the risk factors analyzed and the occurrence of injuries. CONCLUSIONS: Triathlon practice is associated with a high prevalence of injuries, being contusions, knee, and overtraining the most common type, location, and mechanism of injury respectively. It is necessary to create injury prevention strategies, including specific training and suitable materials for use by athletes.

RESUMO INTRODUÇÃO: O triatlo é constituído por três modalidades: natação, ciclismo e corrida. Uma maior frequência de treinamento, fatores ambientais e fatores extrínsecos (técnica e equipamento) podem levar a lesões. O objetivo deste estudo foi determinar a epidemiologia de lesões em triatletas, bem como seu tipo, localização, mecanismo de lesão e fatores de risco. MÉTODOS: A amostra foi constituída por 174 triatletas que participaram do Campeonato Português de Clubes de Triatlo, sendo 131 (75,3%) do sexo masculino, com idades entre 18 e 70 anos (36,09 ± 11,03). O instrumento de medida foi um questionário referente à caracterização da população e aspectos relacionados à modalidade e lesões. RESULTADOS: Cento e vinte (69%) atletas referiram ter tido uma lesão desde que iniciaram a sua prática e 95 (54,6%) sofreram uma lesão no último ano, totalizando 130 lesões. Verificaram-se 2,39 lesões por 1.000 horas de treinamento em triatlo. As lesões mais comuns incluíram contusão muscular (31,5%) e lesões inflamatórias (19,2%), localizadas no joelho (22,3%) e na perna (18,5%). O excesso de treinamento (43,1%) foi o mecanismo que levou à maior ocorrência de lesões. Quatorze (10,8%) lesões ocorreram durante a prática de natação, 23 (17,7%) durante o ciclismo e 93 (71,5%) durante a corrida. Não foi observada significância estatística entre os fatores de risco analisados com a ocorrência de lesões. CONCLUSÕES: A prática de triatlo esteve associada a uma alta prevalência de lesões, sendo a contusão, o joelho e o excesso de treinamento, o tipo, a localização e o mecanismo mais comuns de lesão. É necessário criar estratégias de prevenção de lesões, incluindo treinamento específico e adequação do material utilizado pelo atleta.

SPARC-p53: The double agents of cancer.

Cancer is a complex disease with high incidence and mortality rates. The important role played by the tumor microenvironment in regulating oncogenesis, tumor growth, and metastasis is by now well accepted in the scientific community. SPARC is known to participate in tumor-stromal interactions and impact cancer growth in ambiguous ways, which either enhance or suppress cancer aggressiveness, in a context-dependent manner. p53 transcription factor, a well-established tumor suppressor, has been reported to promote tumor growth in certain situations, such as hypoxia, thus displaying a duality in its action. Although both proteins are being tested in clinical trials, the synergistic relation between them is yet to be explored in clinical practice. In this review, we address the controversial roles of SPARC and p53 as double agents in cancer, briefly summarizing the interaction found between these two molecules and its importance in cancer.

Class IA Phosphatidylinositol 3-Kinase Isoform p110α Mediates Vascular Remodeling.

OBJECTIVE: Neointima formation after vascular injury remains a significant problem in clinical cardiology, and current preventive strategies are suboptimal. Phosphatidylinositol 3'-kinase is a central downstream mediator of growth factor signaling, but the role of phosphatidylinositol 3'-kinase isoforms in vascular remodeling remains elusive. We sought to systematically characterize the precise role of catalytic class IA phosphatidylinositol 3'-kinase isoforms (p110α, p110ß, p110δ), which signal downstream of receptor tyrosine kinases, for vascular remodeling in vivo. APPROACH AND RESULTS: Western blot analyses revealed that all 3 isoforms are abundantly expressed in smooth muscle cells. To analyze their significance for receptor tyrosine kinases-dependent cellular responses, we used targeted gene knockdown and isoform-specific small molecule inhibitors of p110α (PIK-75), p110ß (TGX-221), and p110δ (IC-87114), respectively. We identified p110α to be crucial for receptor tyrosine kinases signaling, thus affecting proliferation, migration, and survival of rat, murine, and human smooth muscle cells, whereas p110ß and p110δ activities were dispensable. Surprisingly, p110δ exerted noncatalytic functions in smooth muscle cell proliferation, but had no effect on migration. Based on these results, we generated a mouse model of smooth muscle cell-specific p110α deficiency (sm-p110α(-/-)). Targeted deletion of p110α in sm-p110α(-/-) mice blunted growth factor-induced cellular responses and abolished neointima formation after balloon injury of the carotid artery in mice. In contrast, p110δ deficiency did not affect vascular remodeling in vivo. CONCLUSIONS: Receptor tyrosine kinases-induced phosphatidylinositol 3'-kinase signaling via the p110α isoform plays a central role for vascular remodeling in vivo. Thus, p110α represents a selective target for the prevention of neointima formation after vascular injury, whereas p110ß and p110δ expression and activity do not play a significant role.