ABSTRACT
Is the most profit to be had from research inspired by curiosity or by foresight of practical applications? A recent suggestion that the answer could be found by evaluating curiosity-oriented projects has proved hard to follow up.
Subject(s)
Galactosidases , Glycoproteins/metabolism , Glycoside Hydrolases , Liver/enzymology , Animals , Cattle , Chemical Phenomena , Chemical Precipitation , Chemistry , Chromatography, Gel , Chromatography, Ion Exchange , Dialysis , Drug Stability , Glucosamine , Hydrogen-Ion Concentration , Kinetics , Mannose , TemperatureABSTRACT
1. A polysaccharide sulphate has been isolated from the hypobranchial mucin of the whelk Buccinum undatum. 2. The molecular weight of this polysaccharide, which is a glucan carrying one ester sulphate group per monosaccharide residue, is 1.7x10(5). 3. Some investigations bearing on the location of the ester sulphate groups are reported. 4. The viscosity of the whole mucin has been shown to depend mainly on the glucan sulphate.
Subject(s)
Mucins , Polysaccharides , Snails , Animals , Chemical Phenomena , Chemistry , In Vitro Techniques , Infrared Rays , Spectrophotometry , Sulfates , UltracentrifugationABSTRACT
1. The presence of beta-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. 2. The purification of beta-acetylglucosaminase (EC 3.2.1.30) and of beta-galactosidase from the ram-testis extract by ammonium sulphate precipitation and chromatography on a CM-cellulose column is described. 3. The final purifications of the separated enzymes achieved were for the beta-acetylglucosaminase 35 times and for the beta-galactosidase 99 times. 4. The possibility of using DEAE-cellulose and Sephadex G-200 to purify the enzymes was investigated.
