ABSTRACT
A new alkaloid, chaerophylline (1), together with known alkaloids (-)-corypalmine, berberine chloride, (-)-isocorypalmine, (-)-corydalmine and (+)-bicuculline have been isolated from the crude base fraction of Corydalis chaerophylla. The crude base fractions of Corydalis longipes gave a new alkaloid, longicine (4), together with known alkaloids (+/-)-alpha-hydrastine, (+/-)-beta-hydrastine, N-methylhydrasteine hydroxylactam, 1-methoxyberberine chloride and berberinium hydroxide. These alkaloids are reported for the first time from the above species and their structures were established by chemical and spectroscopic evidence.
Subject(s)
Alkaloids/chemistry , Corydalis/chemistry , Plant Extracts/chemistry , Alkaloids/isolation & purification , Berberine/analogs & derivatives , Berberine/chemistry , Bicuculline/chemistry , Heterocyclic Compounds, 4 or More Rings/chemistry , Magnetic Resonance Spectroscopy , Molecular StructureABSTRACT
1-Corydalmine,an alkaloid isolated from roots of Corydalis chaerophylla inhibited spore germination of some plant pathogenic as well as saprophytic fungi e.g. Alternaria brassicae, A. brassicicola, A. solani, Curvularia lunata, C. maculans, C. sp., C. pallscens, Erysiphe pisi, Fusarium udum, Helminthosporium species,H. penniseti and a Heterosporium species. 1-Corydalmine significantly inhibited spore germination of all the fungi at 100 to 1500 ppm. It was effective against all the fungi at 1500 ppm. C. lunata was highly sensitive to this chemical even at 250 ppm.
ABSTRACT
(+/-)-alpha-Hydrastine and (+/-)-beta-hydrastine were isolated from Corydalis longipes; both exhibited considerable efficacy against spore germination of some saprophytic and phytopathogenic fungi. While (+/-)-alpha-hydrastine was effective against most of the fungi, Helminthosporium echinoclova was least affected even at the highest dose (150 ppm). (+/-)-beta-Hydrastine was equally effective against several fungi. Mixture of both compounds was more effective than each one individually. Helminthosporium species were again the most resistant toward the mixture. The effect of both alkaloids independently on germination and development of E. pisi conidia on excised pea leaves was also shown. After pre-inoculation with (+/-)-alpha-hydrastine, the effect was more pronounced than the addition post-inoculation; maximum inhibition occurred at 200 ppm. (+/-)-beta-Hydrastine also reduced germination of conidia but was less effective than (+/-)-alpha-hydrastine. The number of primary and secondary branches of conidia and number of appressoria were not affected significantly by either compound.
Subject(s)
Alkaloids/pharmacology , Antifungal Agents/pharmacology , Mitosporic Fungi/drug effects , Plant Diseases/microbiology , Benzylisoquinolines , Corydalis/microbiology , Germination/drug effects , Mitosporic Fungi/growth & development , Pest Control, Biological/methods , Plant Leaves/microbiology , Spores, Fungal/drug effects , Spores, Fungal/growth & developmentABSTRACT
The alkaloids N-methylhydrasteine hydroxylactam and 1-methoxyberberine chloride were isolated from Corydalis longipes. Both alkaloids showed high efficacy individually (in concentration of 50-150 ppm) and also in a 1:1 mixture against spore germination of some fungi, viz. Alternaria alternata, A. brassicae, Curvularia maculans, Curvularia sp., Colletotrichum gloeosporioides, Colletotrichum sp., Helminthosporium speciferum, H. pennisetti, Helminthosporium sp., and Ustilago cynodontis. The antifungal effect of single compounds was dose-dependent. If the mutual ratio of the two components in the mixture was changed from 1:1 to a major content of any of the two compounds, the inhibitory effect on spore germination decreased.
Subject(s)
Alkaloids/chemistry , Antifungal Agents/chemistry , Corydalis/chemistry , Alkaloids/isolation & purification , Alkaloids/pharmacology , Antifungal Agents/isolation & purification , Antifungal Agents/pharmacology , Fungi/drug effects , Fungi/growth & development , Plant Preparations/chemistry , Spores, Fungal/drug effectsABSTRACT
Inhibition activity of the alkaloid (-)-corypalmine on spore germination of plant pathogenic and saprophytic fungi (Alternaria solani, A brassicicola, A. brassicae, A. melongenae, Curvularia pallescens, C. lunata, C. maculans, Curvularisa sp., Colletotrichum sp., Helminthosporium speciferum, H. frumentacei, H. pennisetti, Heterosporium sp., Penicillum sp., Ustilago cynodontis) was determined. Spore germination of all the tested fungi was inhibited, Heterosporium sp. and Ustilago cynodontis being the most sensitive (complete inhibition of spore germination was observed at the very low concentration of 200 ppm). Curvularia palliscens, C. maculans and Curvularia sp. were less sensitive; complete inhibition of spore germination occurred at 400 ppm.
Subject(s)
Alkaloids/chemistry , Alkaloids/pharmacology , Antifungal Agents/pharmacology , Fungi/drug effects , Heterocyclic Compounds, 4 or More Rings/pharmacology , Spores, Fungal/drug effects , Alkaloids/isolation & purification , Antifungal Agents/isolation & purification , Fungi/physiology , Plant Extracts/chemistry , Plant Extracts/pharmacology , Species SpecificityABSTRACT
Berberine and (+/-)-bicuculline were isolated from roots and leaves, respectively, of Corydalis chaerophylla. Both were effective in vitro against spore germination of some plant pathogenic fungi (Alternaria brassicicola, A. brassicae, A. cheiranthi, A. melongenae, A. solani, Colletotrichum musae, C. falcatum, Curvularia penniseti, C. lunata, C. maculans, C. pallescens, Curvularia sp., Erysiphe pisi, E. cichoracearum, Erysiphe sp., Fusarium udum, Helminthosporium spiciferum, H. penniseti, H. frumentacei, Heterosporium sp., Oidium erysiphoides and Ustilago cynodontis). Berberine and (+/-)-bicuculline significantly inhibited spore germination of all the fungi at concentrations of 100-1000 ppm. Berberine was effective against all the fungi at all concentrations; most of the fungi did not germinate at 1000 ppm. H. penniseti conidia did not germinate at any concentration of (+/-)-bicuculline. U. cynodontis was the least sensitive fungus at lower concentrations but 800 ppm dose was highly effective.
Subject(s)
Antifungal Agents/pharmacology , Berberine/pharmacology , Bicuculline/pharmacology , Corydalis/chemistry , Antifungal Agents/isolation & purification , Plants, Medicinal/chemistry , Spores, Fungal/drug effects , Spores, Fungal/growth & developmentABSTRACT
A new triterpene glycoside, arjunetoside, together with oleanolic and arjunic acids has been isolated from the root bark of Terminalia arjuna. The structure of arjunetoside has been established as 3-O-beta-D-glucopyranosyl-2alpha,3beta, 19alpha-trihydroxyolean-12-en-28-oic acid, 28-O-beta-D-glucopyranoside by chemical and spectral data.
Subject(s)
Glycosides/isolation & purification , Plant Extracts/isolation & purification , Terminalia/chemistry , Triterpenes/isolation & purification , Chromatography, Thin Layer , Glycosides/chemistry , India , Magnetic Resonance Spectroscopy , Mass Spectrometry , Methylation , Molecular Structure , Oleanolic Acid/chemistry , Oleanolic Acid/isolation & purification , Plant Extracts/chemistry , Plant Roots/chemistry , Plants, Medicinal/chemistry , Spectrophotometry, Infrared , Triterpenes/chemistryABSTRACT
From the whole plant of Eclipta alba, a new triterpene saponin, named eclalbatin, together with alpha-amyrin, ursolic acid and oleanolic acid were isolated. The structure of eclalbatin has been established as 3-O-beta-D-glucopyranosyl-3-beta-hydroxy-olean-12-en-28-oic acid, 28-O-beta-D-arabinopyranoside (1) on the basis of chemical and spectral data.
Subject(s)
Asteraceae/chemistry , Plant Extracts/isolation & purification , Saponins/isolation & purification , Triterpenes/isolation & purification , Chromatography, Thin Layer , India , Mass Spectrometry , Medicine, Traditional , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Oleanolic Acid/chemistry , Oleanolic Acid/isolation & purification , Plant Extracts/chemistry , Plants, Medicinal/chemistry , Saponins/chemistry , Spectrophotometry, Infrared , Triterpenes/chemistry , Ursolic AcidABSTRACT
A new cyclopeptide alkaloid, jubanine-C (1), together with known alkaloids scutianine-C (4) and zizyphine-A (5), have been isolated from the stem bark of Zizyphus jujuba and identified by spectral analysis.
