ABSTRACT
Amongst the variety of oceanic processes running the gamut of spatiotemporal scales, mesoscale eddies are the most common and often have region-specific characteristics. The large kinetic energy inherent to eddies themselves is a strong modulator of the global climate, ocean circulation, productivity, and freshwater transport. This study uses multi-source satellite remote sensing observation data to construct a multi-parameter eddy dataset for the 1993-2019 period, which differs significantly from a few of previous published eddy datasets that include only basic sea surface eddy physical features. Eddies within the dataset have life cycles of greater than four weeks, and their corresponding sea surface chlorophyll, sea surface temperature, and wind fields are provided. Atmospheric and oceanic variables are used to present a comprehensive picture of a given mesoscale eddy's impact on the local physical, but also biological environment. The dataset would find immense value in research on mesoscale eddies, their impact on the atmosphere, and related biological processes.
ABSTRACT
Polian vesicles are considered as the site of coelomocyte formation, and play crucial roles in the inflammatory reaction in sea cucumber. After evisceration, coelomocytes and internal organs except polian vesicles are excreted. Our previous study found that the total number of coelomocytes was rapidly recovered at 6 h post-evisceration in sea cucumber Apostichopus japonicus, and this regeneration of coelomocytes might be closely related to polian vesicles. To further investigate the related-gene expression pattern of the polian vesicles at 6 h post-evisceration, the transcriptome analysis of polian vesicles was carried out. A total of 2752 differentially expressed genes (DEGs) were identified, including 1,453 up-regulated genes and 1299 down-regulated genes. Gene Ontology (GO) enrichment showed that most of the DEGs were classified under Regulation of transcription, Regulation of RNA metabolic process, Regulation of nucleic acid-templated transcription. Meanwhile, 11 significantly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified. Among them, Wnt, TGF-ß and Endocytosis pathways are well-related with cell proliferation and differentiation, which may be involved in the regeneration of coelomocytes in A. japonicus after evisceration. In addition, FoxO signaling pathway plays important roles in immunoregulation, in which the expression levels of the DEGs were significantly up-regulated, inferring that polian vesicles could not only participate in the coelomocyte regeneration process, but also undertake a certain immune defense function in A. japonicus after evisceration. These findings will be beneficial for understanding the mechanisms of coelomocyte regeneration and immune defense of A. japonicus after evisceration.
