ABSTRACT
Pancreatic cancer is a malignant tumor of the digestive system that is highly malignant, difficult to treat, and confers a poor prognosis for patients. BRAF-activated noncoding RNA (BANCR) has been proven to play an important role in the invasion and metastasis of pancreatic cancer. In this study, we focused on BANCR as a potential therapeutic target for human pancreatic cancer. The BANCR level in pancreatic cancer tissues and cells is affected by m6A methylation. Based on this, the aim of our study was to investigate the effect of a highly potent and selective first-in-class catalytic inhibitor of METTL3 (STM2457) on BANCR m6A methylation and its malignant biological behaviors in pancreatic cancer. The relationship between BANCR expression and BANCR m6A modification was detected with RT-qPCR and MeRIP-PCR. The expression of methyltransferase-like 3 (METTL3), the key enzyme involved in m6A methylation, in pancreatic cancer tissues was detected using a Western blot. STM2457 was used in vitro to investigate its resistance to the proliferation, invasion, and metastasis of pancreatic cancer cells. BANCR was overexpressed in pancreatic cancer tissues and cells, which was associated with poor clinical outcomes and validated in pancreatic cancer cell lines. m6A modification was highly enriched within BANCR and enhanced its expression. Remarkably, STM2457 inhibited the proliferation, invasion, and metastasis of pancreatic cancer cells by down-regulating BANCR m6A modifications. This study demonstrates the promise of BANCR as a new diagnostic and therapeutic target for pancreatic cancer and reveals the therapeutic effect that STM2457 exerts on pancreatic cancer by down-regulating BANCR m6A modifications.
ABSTRACT
Flexible and wearable chemical sensors show great capability and potential in retrieving physiologically related chemical or biochemical information from elastic and curvilinear living bodies. However, so far, no flexible electrochemiluminescence (ECL) device has been reported, though ECL measurements have been extensively investigated and widely applied in many fields. Herein, we for the first time designed and fabricated a flexible ECL sensor by immobilizing highly luminescent nanospheres on Au nanotube (Au NT) networks, and subsequently coating an elastic molecularly imprinted polymer (MIP) thereon. The as-prepared flexible ECL platform displayed successive and desirable mechanical compliance while generating a very stable ECL signal during deformation, facilitating highly selective detection of physiologically relevant chemicals from bodies. On-body wearable sampling and subsequent detection of lactate and urea from sweat showed the ECL performance of this sensor displaying desirable fidelity, reusability and high stability against disturbance. This work successfully incorporated the ECL sensing model into a flexible and wearable device, therefore providing a promising new path for non-invasively monitoring the products of metabolism for health care and biomedical investigations.
ABSTRACT
A rapid and ultrasensitive molecularly imprinted photoelectrochemical (MIP-PEC) sensing platform based on ITO electrode modified with GO-CdS heterojunction was prepared for ultrasensitive measure of fumonisin B1 (FB1). CdS quantum dots (QDs) were combined with a suitable amount of graphene oxide (GO) to form a heterojunction to enhance signal response with accurately calculating energy levels (VB/CB or HOMO/LUMO). The MIP-PEC sensor was successful fabricated after MIP was immobilized on the electrode with the basis of these results. In the phosphate buffer solution (PBS), it was clearly observed that the non-elution MIP-PEC sensor had almost no photocurrent response, which was due to the slower electron transfer speed. When the MIP-PEC sensor is eluted in ethanol, its photocurrent response was significantly restored, that was because the fact that the template molecules were washed away, and electron donors entered the holes and accelerated the electron transfer. Its photocurrent response was reduced because of holes blocked when the MIP-PEC sensor was hatched in the template molecules culture fluid. This phenomenon fully showed that the MIP-PEC sensor can specifically detect the target. Thus, The work has a linear range from 0.01 to 1000â¯ngâ¯mL-1 with a detection limit of 4.7â¯pgâ¯mL-1 for FB1. Furthermore, the fabricated MIP-PEC sensor will confirm the actual application.
