Diet Change Improves Obesity and Lipid Deposition in High-Fat Diet-Induced Mice.

The number of obese people is increasing dramatically worldwide, and one of the major causes of obesity is excess energy due to high-fat diets. Several studies have shown that reducing food and energy intake represents a key intervention or treatment to combat overweight/obesity. Here, we conducted a 12-week energy-restricted dietary intervention for high-fat diet-induced obese mice (C57BL/6J) to investigate the effectiveness of diet change in improving obesity. The results revealed that the diet change from HFD to NFD significantly reduced weight gain and subcutaneous adipose tissue weight in high-fat diet-induced obese mice, providing scientific evidence for the effectiveness of diet change in improving body weight and fat deposition in obese individuals. Regarding the potential explanations for these observations, weight reduction may be attributed to the excessive enlargement of adipocytes in the white adipose tissue of obese mice that were inhibited. Diet change significantly promoted lipolysis in the adipose tissue (eWAT: Adrb3, Plin1, HSL, and CPTA1a; ingWAT: CPT1a) and liver (reduced content of nonesterified fatty acids), and reduced lipogenesis in ingWAT (Dgat2). Moreover, the proportion of proliferative stem cells in vWAT and sWAT changed dramatically with diet change. Overall, our study reveals the phenotypic, structural, and metabolic diversity of multiple tissues (vWAT and sWAT) in response to diet change and identifies a role for adipocyte stem cells in the tissue specificity of diet change.

Succinate Enhances Lipolysis and Decreases Adipocytes Size in Both Subcutaneous and Visceral Adipose Tissue from High-Fat-Diet-Fed Obese Mice.

Obesity is a risk factor for many chronic diseases related to the overexpansion of adipose tissue during obesity, leading to metabolic dysfunction and ectopic lipids. Previous studies reported a close relationship between succinate and obesity and its co-morbidities, and studies have also reported on its anti-obesity potential. To confirm its efficacy in obesity interventions, we supplemented mice with obesity induced by a high-fat diet with succinate (1.5% m/v in drinking water) for 11 weeks without changing the diet. After succinate supplementation, the changes in body weight, adipose tissue deposition, glucose tolerance, energy expenditure and lipid metabolism were evaluated. It was found that succinate supplementation significantly decreased subcutaneous adipose tissue (HFD: 4239.3 ± 211.2 mg; HFD-SA: 3268.9 ± 265.7 mg. p < 0.05), triglyceride contents (decreased by 1.53 mmol/g and 0.39 mmol/g in eWAT and ingWAT, respectively, p < 0.05) and NEFA (decreased by 1.41 µmol/g and 1.31 µmol/g in eWAT and ingWAT, respectively, p < 0.05). The adipocytes' sizes all significantly decreased in both subcutaneous and visceral adipose tissue (the proportion of adipocytes with diameters larger than 100 µm in eWAT and ingWAT decreased by 16.83% and 11.96%, respectively. p < 0.05). Succinate significantly enhanced lipolysis in adipose tissue (eWAT: Adrb3, Hsl and Plin1; ingWAT: Hsl and CPT1a; p < 0.05), whereas the expression of lipogenesis-related genes remained unchanged (p > 0.05). Succinate supplementation also enhanced the activity of BAT by stimulating the expression of Ucp1 and Cidea (p < 0.05). Our results reported that succinate has a potential beneficial effect on obesity pathogenesis but cannot efficiently decrease bodyweight.

Improving nisin production by encapsulated Lactococcus lactis with starch/carboxymethyl cellulose edible films.

In this study, Lactococcus lactis was embedded in a film of corn starch (NS) and carboxymethyl cellulose (CMC) prepared using a casting method. At a CMC:NS ratio of 5:5, the composite film had the best comprehensive properties. Scanning electron microscopy images clearly showed that L. lactis was effectively embedded. The film with 1.5 % L. lactis showed the best performance and the lowest water vapor transmission rate (5.54 × 10-11 g/m s Pa. In addition, the edible film retained a viable count of 5.64 log CFU/g of L. lactis when stored at 4 °C for 30 days. The composite film with 1.5 % L. lactis showed the highest release of nisin (3.35 mg/mL) and good antibacterial activity against Staphylococcus aureus (53.53 %) after 8 days. Therefore, this edible film is a viable alternative antimicrobial strategy for the active packaging of foods containing low moisture content.

Effects of Ultrasonication Time on the Properties of Polyvinyl Alcohol/Sodium Carboxymethyl Cellulose/Nano-ZnO/Multilayer Graphene Nanoplatelet Composite Films.

Ultrasonication-assisted solution casting was used to prepare polyvinyl alcohol (PVA)/sodium carboxymethyl cellulose (CMC)/nano-ZnO/multilayer graphene nanoplatelet (xGnP) composite films; the performances (mechanical properties, water vapor permeability (WVP), biodegradability and antibacterial activity) of these films were investigated as a function of the ZnO NPs:xGnP mass ratio and ultrasonication time. Intermolecular interactions among ZnO NPs, xGnP and the PVA/CMC matrix were shown to improve WVP, while X-ray diffraction and scanning electron microscopy analyses revealed that the internal reticular structure of ultrasound-treated PVA/CMC/ZnO NPs/xGnP composite films was in a fluffier state than that of the untreated composite films and the PVA/CMC film. The incorporation of ZnO NPs and xGnP into the composite film reduced its tensile strength and elongation at break, and increased antibacterial activity and biodegradability. In addition, we carried out the experiment of strawberry preservation and measured weight loss ratio, firmness, content of total soluble solids and titration acid. Finally, the composite film of 7:3 had the best preservation effect on strawberries. Thus, the obtained results paved the way to develop novel biodegradable composite films with antimicrobial activity for a wide range of applications.

Long-Term Antibacterial Effect of Electrospun Polyvinyl Alcohol/Polyacrylate Sodium Nanofiber Containing Nisin-Loaded Nanoparticles.

Response Surface Methodology (RSM) was used to assess the optimal conditions for a Water/Oil/Water (W/O/W) emulsion for encapsulated nisin (EN). Nano-encapsulated nisin had high encapsulation efficiencies (EE) (86.66 ± 1.59%), small particle size (320 ± 20 nm), and low polydispersity index (0.27). Biodegradable polyvinyl alcohol (PVA) and polyacrylate sodium (PAAS) were blended with EN and prepared by electrospinning. Scanning electron microscopy (SEM) revealed PVA/PAAS/EN nanofibers with good morphology, and that their EN activity and mechanical properties were enhanced. When the ultrasonication time was 15 min and 15% EN was added, the nanofibers had optimal mechanical, light transmittance, and barrier properties. Besides, the release behavior of nisin from the nanofibers fit the Korsemeyer-Peppas (KP) model, a maximum nisin release rate of 85.28 ± 2.38% was achieved over 16 days. At 4 °C, the growth of Escherichia coli and Staphylococcus aureus was inhibited for 16 days in nanofibers under different ultrasonic times. The application of the fiber in food packaging can effectively inhibit the activity of food microorganisms and prolong the shelf life of strawberries, displaying a great potential application for food preservation.

Cassava starch/carboxymethylcellulose edible films embedded with lactic acid bacteria to extend the shelf life of banana.

In this study, two different species of lactic acid bacteria (LAB) (Lactobacillus plantarum and Pedocococcus pentosaceus) with high exopolysaccharide (EPS) yield were selected from a pickled water. Further, edible films based on cassava starch (CS) were developed containing LAB, and sodium carboxymethylcellulose (CMC). After addition of probiotics, the antioxidant activity of the composite film was significantly enhanced. Further, as the probiotic content increased, the antioxidant activity continuously increased. Moreover, L. plantarum showed uniform distribution in the CS/CMC matrix, forming a denser structure, which effectively blocked the penetration of water molecules and exhibited ultraviolet protection. Finally, due to the intermolecular interaction between probiotics and the CS/CMC matrix, the water vapor and light transmission rates were reduced, effectively blocking water and light to prevent lipid oxidation deterioration in food packaging. Banana shelf life has qualitatively improved with 2% LAB added composite film and its application in food packaging has been affirmed.

Development and optimization of dynamic gelatin/chitosan nanoparticles incorporated with blueberry anthocyanins for milk freshness monitoring.

In this study, we prepared gelatin and chitosan as wall materials, and composites with a controlled release capability in a weak acidic environment were synthesized for loading and protecting anthocyanins. Fourier-transform infrared spectroscopy and scanning electron microscopy were used to assess the properties of the nanoparticles. The loading efficiencies and oxidation resistances of the proposed substances were measured. Under optimal conditions, the anthocyanins exhibited a loading efficiency of 83.81 %, and suitable long-term storage capacity at room temperature with a retention rate of ∼50 % after 15 d. When the nanoparticles were used for detecting the milk freshness, spoiled milk exhibited a reddish color, whereas the color of fresh milk did not change. Moreover, these nanoparticles exhibited a stable chroma in milk for 0-16 h. Therefore, the proposed pH responsive nanoparticles can provide a possibility for the dynamic monitoring of milk quality changes, or provide some reference value for future related research.