Correction of a transcranial acoustic field using a transient ultrasound field visualization technique.

Ultrasound, due to its noninvasive nature, has the potential to enhance or suppress neural activity, making it highly promising for regulating intractable brain disorders. Precise ultrasound stimulation is crucial for improving the efficiency of neural modulation and studying its mechanisms. However, the presence of the skull can cause distortion in the ultrasound field, thereby affecting the accuracy of stimulation. Existing correction methods primarily rely on magnetic resonance guidance and numerical simulation. Due to the large size and high cost, the MR-guided transcranial ultrasound is difficult to be widely applied in small animals. The numerical simulation usually requires further validation and optimization before application, and the most effective method is to visualize the excited ultrasound field. However, the ultrasound field correction methods based on acoustic field visualization are still lacking. Therefore, a shadowgraph-based transient ultrasonic field visualization system is developed, and an ex vivo transcranial ultrasound field correction is performed. By visualizing the ultrasound field with or without a rat skull and then calculating the time difference of each element's ultrasound wavefront, the parameters for ultrasound field correction can be achieved. The experimental results show that this method can improve both the shape and the size of the focal spot, as well as enhance the acoustic pressure at the focus. Overall, the results demonstrate that the ultrasonic field visualization technology can effectively improve the transcranial ultrasound focusing effect and provide a new tool for achieving precise ultrasonic neural modulation.

Micromachining of High-Quality PMN-PT/Epoxy 1-3 Composite for High-Frequency (>30 MHz) Ultrasonic Transducer Applications.

Fabricating PMN-PT composites, the core component of high-frequency (> 30 MHz) transducers, remains challenging due to their poor machinability and ultrasmall kerfs. This urgent problem is significantly impeding the development of PMN-PT ultrasonic transducers for use in clinical research, biomedical sciences, and nondestructive testing (NDT). In this study, high-quality PMN-0.3PT/epoxy 1-3 composites at 30 and 50 MHz were manufactured using a modified picosecond (1.5 ps) laser technique. Their performance was thoroughly analyzed, which was comparable to that with low-stress dry plasma etching. There were fewer microcracks around PMN-PT pillars. The minimum kerf was less than [Formula: see text], and the highest aspect ratio was larger than 7.5. The microdomain morphology and hysteresis loops of PMN-PT pillars further confirmed that composites still maintained excellent piezoelectric performance and suffered fewer damages during laser cutting. The characterization results exhibited a large electromechanical coupling (>0.77), a high dielectric constant (>1600), and a relatively low acoustic impedance (< 17 Mrayls). The ultrasonic transducers with center frequencies of 30 and 50 MHz were designed and prototyped to validate the performance of composites. The transducers showed broad bandwidth (>80%), high two-way insertion loss (IL) (>-23 dB), and imaging resolution superior to [Formula: see text]. Finally, the C-scan experiments of IC chips were also used to further illustrate the applicability of transducers. These encouraging results further demonstrated that ultrafast laser technology will bring more accessible and affordable methods for fabricating high-frequency PMN-PT composite transducers with excellent performance.

Transcranial ultrasound neuromodulation facilitates isoflurane-induced general anesthesia recovery and improves cognition in mice.

Delayed arousal and cognitive dysfunction are common, especially in older patients after general anesthesia (GA). Elevating central nervous system serotonin (5-HT) levels can promote recovery from GA and increase synaptic plasticity to improve cognition. Ultrasound neuromodulation has become a noninvasive physical intervention therapy with high spatial resolution and penetration depth, which can modulate neuronal excitability to treat psychiatric and neurodegenerative diseases. This study aims to use ultrasound to noninvasively modulate the brain 5-HT levels of mice to promote recovery from GA and improve cognition in mice. The dorsal raphe nucleus (DRN) of mice during GA was stimulated by the 1.1 MHz ultrasound with a negative pressure of 356 kPa, and the liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) method was used to measure the DRN 5-HT concentrations. The mice's recovery time from GA was assessed, and the cognition was evaluated through spontaneous alternation Y-maze and novel object recognition (NOR) tests. After ultrasound stimulation, the mice's DRN 5-HT levels were significantly increased (control: 554.0 ± 103.2 ng/g, anesthesia + US: 664.2 ± 84.1 ng/g, *p = 0.0389); the GA recovery time (return of the righting reflex (RORR) emergence latency time) of mice was significantly reduced (anesthesia: 331.6 ± 70 s, anesthesia + US: 223.2 ± 67.7 s, *p = 0.0215); the spontaneous rotation behavior score of mice was significantly increased (anesthesia: 59.46 ± 5.26 %, anesthesia + US: 68.55 ± 5.24 %; *p = 0.0126); the recognition index was significantly increased (anesthesia: 55.02 ± 6.23 %, anesthesia + US: 78.52 ± 12.21 %; ***p = 0.0009). This study indicates that ultrasound stimulation of DRN increases serotonin levels, accelerates recovery from anesthesia, and improves cognition, which could be an important strategy for treating delayed arousal, postoperative delirium, or even lasting cognitive dysfunction after GA.

Ultrasound neuromodulation ameliorates chronic corticosterone-induced depression- and anxiety-like behaviors in mice.

Objective.Monoamine dysfunction has been implicated as a pathophysiological basis of several mental disorders, including anxiety and depression. Transcranial ultrasound stimulation (TUS) is a noninvasive nerve stimulation technic showing great potential in treating depression/anxiety disorders. This study aims to investigate whether TUS can ameliorate depression with anxiety in mice by regulating brain monoamine levels.Approach.Mice received repeated subcutaneous injections of corticosterone (CORT, 20 mg kg-1) for 3 weeks to produce depression- and anxiety-like behaviors. Ultrasound stimulated the dorsal lateral nucleus (DRN) for 30 min daily for 3 weeks without interruption of CORT injection. Behavioral phenotypes of depression and anxiety were estimated by sucrose preference test (SPT), tail suspension test (TST), and elevated plus-maze test (EPM). Liquid chromatography-mass spectrometry (LC-MS) was used to quantify brain levels of serotonin (5-HT), norepinephrine (NE), and dopamine (DA). Western blotting was performed to detect brain-derived neurotrophic factor (BDNF) levels in hippocampal.Main results.TUS of DRN significantly ameliorated the depression-like behaviors in SPT (p= 0.0004) and TST (p= 0.0003) as well as anxiety-like behaviors in EPM (open arm entry frequencies,p< 0.05). Moreover, TUS increased c-Fos-positive cell expression (p= 0.0127) and induced no tissue damage. LC-MS results showed TUS of DRN resulted in a non-significant increase in the 5-HT levels and a significant decrease in the NE levels, but did not affect the levels of DA and BDNF.Significance.These results suggest TUS of DRN has safely and effectively ameliorated CORT-induced depression- and anxiety-like behaviors, possibly by restoring brain levels of 5-HT and NE. TUS may be a safe and effective technique for remedying depression and anxiety comorbidity.

Photoacoustic maximum amplitude projection microscopy by ultra-low data sampling.

Photoacoustic microscopy (PAM) has attracted increasing research interest in the biomedical field due to its unique merit of combining light and sound. In general, the bandwidth of a photoacoustic signal reaches up to tens or even hundreds of MHz, which requires a high-performance acquisition card to meet the high requirement of precision of sampling and control. For most depth-insensitive scenes, it is complex and costly to capture the photoacoustic maximum amplitude projection (MAP) images. Herein, we propose a simple and low-cost MAP-PAM system based on a custom-made peak holding circuit to obtain the extremum values by Hz data sampling. The dynamic range of the input signal is 0.01-2.5 V, and the -6-dB bandwidth of the input signal can be up to 45 MHz. Through in vitro and in vivo experiments, we have verified that the system has the same imaging ability as conventional PAM. Owing to its compact size and ultra-low price (approximately $18), it provides a new performance paradigm for PAM and opens up a new way for an optimal photoacoustic sensing and imaging device.

Ultrasonic nondestructive evaluation of the bonding strength of polyurethane coatings based on feedforward comb filtering effect.

Because of the ability to protect and decorate materials, polyurethane coatings (PU) are being applied more and more extensively in the transportation, furniture, automotive, and electronic industries. However, it is difficult to effectively evaluate the bonding quality of coatings by conventional destructive methods for high-volume products in industrial applications. In this work, an ultrasonic nondestructive method based on the feedforward comb filtering effect was proposed to assess the bonding strength of the PU coatings. The coating and substrate interface can be modeled as a distributed spring system. The phase of the reflected ultrasonic wave is highly related to the interfacial stiffness. The superimposed signals can be well analyzed based on the comb filtering effect, and an index called the ratio of notch frequencies to interval frequencies (NIR) was constructed to represent the phase difference. Finite element simulations were conducted to show the index's construction method and variation with different interfacial stiffnesses. Immersed ultrasonic testing experiments with a transducer of 39.43 MHz were then conducted on PU coatings on two kinds of substrates made of aluminum and magnesium alloys. Each specimen was scanned as a grid to mimic the procedure of the well-known cross-cut test. The notch frequencies of the scanned received signals were applied to obtain the NIR index. To avoid the interference of the thickness of coatings, the decimal part of NIR was further proposed to link with the bonding strength obtained by the cross-cut test. This index changes accordingly with aluminum- and magnesium-based coatings' bonding strength. The results prove that the proposed ultrasonic method based on the feedforward comb filter effect is high potential to assess the bonding strength of coatings nondestructively.

Simultaneous multi-target ultrasound neuromodulation in freely-moving mice based on a single-element ultrasound transducer.

Objective.Ultrasound neuromodulation has become an emerging method for the therapy of neurodegenerative and psychiatric diseases. The phased array ultrasonic transducer enables multi-target ultrasound neuromodulation in small animals, but the relatively large size and mass and the thick cables of the array limit the free movement of small animals. Furthermore, spatial interference may occur during multi-target ultrasound brain stimulation with multiple micro transducers.Approach.In this study, we developed a miniature power ultrasound transducer and used the virtual source time inversion method and 3D printing technology to design, optimize, and manufacture the acoustic holographic lens to construct a multi-target ultrasound neuromodulation system for free-moving mice. The feasibility of the system was verified byin vitrotranscranial ultrasound field measurements,in vivodual-target blood-brain barrier (BBB) opening experiments, andin vivodual-target ultrasound neuromodulation experiments.Main results.The developed miniature transducer had a diameter of 4.0 mm, a center frequency of 1.1 MHz, and a weight of 1.25 g. The developed miniature acoustic holographic lens had a weight of 0.019 g to generate dual-focus transcranial ultrasound. The ultrasonic field measurements' results showed that the bifocal's horizontal distance was 3.0 mm, the -6 dB focal spot width in thex-direction was 2.5 and 2.25 mm, and 2.12 and 2.24 mm in they-direction. Finally, thein vivoexperimental results showed that the system could achieve dual-target BBB opening and ultrasound neuromodulation in freely-moving mice.Significance.The ultrasonic neuromodulation system based on a miniature single-element transducer and the miniature acoustic holographic lens could achieve dual-target neuromodulation in awake small animals, which is expected to be applied to the research of non-invasive dual-target ultrasonic treatment of brain diseases in awake small animals.

Transcranial ultrasound stimulation relieves depression in mice with chronic restraint stress.

Objective.Exhaustion of Serotonin (5-hydroxytryptamine, 5-HT) is a typical cause of the depression disorder's development and progression, including depression-like behaviors. Transcranial ultrasound stimulation (TUS) is an emerging non-invasive neuromodulation technique treating various neurodegenerative diseases. This study aims to investigate whether TUS ameliorates depression-like behaviors by restoring 5-HT levels.Methods.The depression model mice are established by chronic restraint stress (CRS). Ultrasound waves (FF = 1.1 MHz, PRF = 1000 Hz, TBD = 0.5 ms, SD = 1 s, ISI = 1 s, and DC = 50%) were delivered into the dorsal raphe nucleus (DRN) for 30 min per day for 2 weeks. Depression-like behavior changes are evaluated with the sucrose preference and tail suspension tests. Liquid chromatography-mass spectrometry is performed to quantitatively detect the concentration of 5-HT in the DRN to explore its potential mechanism. The effectiveness and safety of TUS were assessed by c-Fos immunofluorescence and hematoxylin and eosin (HE) staining, respectively.Results.Three weeks after CRS, 22 depressive mice models were screened by sucrose preference index (SPI). After 2 weeks of ultrasound stimulation of the DRN (DRN-TUS) in depressive mice, the SPI was increased (p= 0.1527) and the tail suspension immobility duration was significantly decreased (p= 0.0038) compared with the non-stimulated group. In addition, TUS significantly enhances the c-Fos (p= 0.05) positive cells' expression and the 5-HT level (p= 0.0079) in the DRN. Importantly, HE staining shows no brain tissue damage.Conclusion.These results indicate that DRN-TUS has safely and effectively improved depression-like behaviors including anhedonia and hopelessness, potentially by reversing the depletion of 5-TH.SignificanceTUS may provide a new perspective on depression therapy, possibly through restoring monoamine levels.

A Spatial Multitarget Ultrasound Neuromodulation System Using High-Powered 2-D Array Transducer.

Transcranial focused ultrasound (tFUS) is increasingly used in experimental neuroscience due to its neuromodulatory effectiveness in animal studies. However, achieving multitarget tFUS in small animals is typically limited by transducer size, energy transfer efficiency, and brain volume. The objective of this work was to construct an ultrasound system for multitarget neuromodulation in small animals. First, a miniaturized high-powered 2-D array transducer was developed. The phase delay of each array element was calculated based on the multifocal time-reversal method, generating multiple foci simultaneously in a 3-D field. The effects of the axial focal length, interfocus spacing (lateral distance between the two focal centers), and the number of foci on the focal properties of the pressure field were examined through numerical simulations. In-vitro ultrasonic measurements and transcranial simulations on a rat skull were conducted. The minimum interfocus spacing separating two -6-dB foci and the peak full-width at half-maximum were positively correlated with axial focal length; the relative relationship between the interfocus spacing and pressure field properties was similar for each axial focal length. The maximum acoustic pressure and spatial average intensity at focus in deionized water were 2.21 MPa and 133 W/cm2, respectively. The simulated and experimental results were compared, demonstrating agreement in both peak position and focus shape. The ultrasound system can provide a neuroscientific platform for evaluating the feasibility of multitarget ultrasound stimulation treatment protocols, thus improving the understanding of functional neuroanatomy.

Multitarget Transcranial Ultrasound Therapy in Small Animals Based on Phase-Only Acoustic Holographic Lens.

Transcranial ultrasound therapy has become a noninvasive method for treating neurological and psychiatric disorders, and studies have further demonstrated that multitarget transcranial ultrasound therapy is a better solution. At present, multitarget transcranial ultrasound therapy in small animals can only be achieved by the multitransducer or phased array. However, multiple transducers may cause spatial interference, and the phased array system is complicated, expensive, and especially unsuitable for small animals. This study is the first to design and fabricate a miniature acoustic holography lens for multitarget transcranial ultrasound therapy in rats. The acoustic holographic lens, working at a frequency of 1.0 MHz, with a size of 10.08 mm ×10.08 mm and a pixel resolution of 0.72 mm, was designed, optimized, and fabricated. The dual-focus transcranial ultrasound generated based on the lens was measured; the full-width at half-maximum (FWHM) of the focal spots in the y -direction was 2.15 and 2.27 mm and in the z -direction was 2.3 and 2.36 mm. The focal length was 5.4 mm, and the distance between the two focuses was 5.6 mm, close to the desired values of 5.4 and 6.0 mm. Finally, the multiple-target blood-brain barrier opening in rats' bilateral secondary visual cortex (mediolateral area, V2ML) was demonstrated using the transcranial ultrasound therapy system based on the lens. These results demonstrate the good performance of the multitarget transcranial ultrasound therapy system for small animals, including high spatial resolution, small size, and low cost.

Numerical Study on Surface Roughness Measurement Based on Nonlinear Ultrasonics in Through-Transmission and Pulse-Echo Modes.

Ultrasonic is one of the well-known methods for surface roughness measurement, but small roughness will only lead to a subtle variation of transmission or reflection. To explore sensitive techniques for surfaces with small roughness, nonlinear ultrasonic measurement in through-transmission and pulse-echo modes was proposed and studied based on an effective unit-cell finite element (FE) model. Higher harmonic generation in solids was realized by applying the Murnaghan hyperelastic material model. This FE model was verified by comparing the absolute value of the nonlinearity parameter with the analytical solution. Then, random surfaces with different roughness values ranging from 0 µm to 200 µm were repeatedly generated and studied in the two modes. The through-transmission mode is very suitable to measure the surfaces with roughness as small as 3% of the wavelength. The pulse-echo mode is sensitive and effective to measure the surface roughness ranging from 0.78% to 5.47% of the wavelength. This study offers a potential nondestructive testing and monitoring method for the interfaces or inner surfaces of the in-service structures.

Ultrasonic Particle Manipulation in Glass Capillaries: A Concise Review.

Ultrasonic particle manipulation (UPM), a non-contact and label-free method that uses ultrasonic waves to manipulate micro- or nano-scale particles, has recently gained significant attention in the microfluidics community. Moreover, glass is optically transparent and has dimensional stability, distinct acoustic impedance to water and a high acoustic quality factor, making it an excellent material for constructing chambers for ultrasonic resonators. Over the past several decades, glass capillaries are increasingly designed for a variety of UPMs, e.g., patterning, focusing, trapping and transporting of micron or submicron particles. Herein, we review established and emerging glass capillary-transducer devices, describing their underlying mechanisms of operation, with special emphasis on the application of glass capillaries with fluid channels of various cross-sections (i.e., rectangular, square and circular) on UPM. We believe that this review will provide a superior guidance for the design of glass capillary-based UPM devices for acoustic tweezers-based research.

Centimeter-scale wide-field-of-view laser-scanning photoacoustic microscopy for subcutaneous microvasculature in vivo.

We developed a simple and compact laser-scanning photoacoustic microscopy (PAM) for imaging large areas of subcutaneous microvasculature in vivo. The reflection-mode PAM not only retains the advantage of high scanning speed for optical scanning, but also offers an imaging field-of-view (FOV) up to 20 × 20 mm2, which is the largest FOV available in laser-scanning models so far. The lateral resolution of the PAM system was measured to be 17.5 µm. Image experiments on subcutaneous microvasculature in in vivo mouse ears and abdomen demonstrate the system's potential for fast and high-resolution imaging for injuries and diseases of large tissues and organs.

X-Ray-Induced Acoustic Computed Tomography (XACT): Initial Experiment on Bone Sample.

X-ray-induced acoustic computed tomography (XACT) is a unique hybrid imaging modality that combines high X-ray absorption contrast with high ultrasonic resolution. X-ray radiography and computerized tomography (CT) are currently the gold standards for 2-D and 3-D imaging of skeletal tissues though there are important properties of bone, such as elasticity and speed of sound (SOS), that these techniques cannot measure. Ultrasound is capable of measuring such properties though current clinical ultrasound scanners cannot be used to image the interior morphology of bones because they fail to address the complicated physics involved for exact image reconstruction; bone is heterogeneous and composed of layers of both cortical and trabecular bone, which violates assumptions in conventional ultrasound imaging of uniform SOS. XACT, in conjunction with the time-reversal algorithm, is capable of generating precise reconstructions, and by combining elements of both X-ray and ultrasound imaging, XACT is potentially capable of obtaining more information than any single of these techniques at low radiation dose. This article highlights X-ray-induced acoustic detection through linear scanning of an ultrasound transducer and the time-reversal algorithm to produce the first-ever XACT image of a bone sample. The results of this study should prove to enhance the potential of XACT imaging in the evaluation of bone diseases for future clinical use.

Micromachining of High Quality PMN-31%PT Single Crystals for High-Frequency (>20 MHz) Ultrasonic Array Transducer Applications.

A decrease of piezoelectric properties in the fabrication of ultra-small Pb(Mg1/3Nb2/3)-x%PbTiO3 (PMN-x%PT) for high-frequency (>20 MHz) ultrasonic array transducers remains an urgent problem. Here, PMN-31%PT with micron-sized kerfs and high piezoelectric performance was micromachined using a 355 nm laser. We studied the kerf profile as a function of laser parameters, revealing that micron-sized kerfs with designated profiles and fewer micro-cracks can be obtained by optimizing the laser parameters. The domain morphology of micromachined PMN-31%PT was thoroughly analyzed to validate the superior piezoelectric performance maintained near the kerfs. A high piezoresponse of the samples after micromachining was also successfully demonstrated by determining the effective piezoelectric coefficient (d33*~1200 pm/V). Our results are promising for fabricating superior PMN-31%PT and other piezoelectric high-frequency (>20 MHz) ultrasonic array transducers.

3D Printing of BaTiO3 Piezoelectric Ceramics for a Focused Ultrasonic Array.

BaTiO3 (BTO) ceramics were fabricated based on stereolithography technology. The microstructures and electric properties of the BTO ceramics were studied. X-ray patterns of sintered BTO ceramics indicated that the tetragonal phase had formed, and the grain size increased clearly as BTO weight percentage increased. Moreover, the BTO ceramics exhibited good electric properties, with a piezoelectric constant d33 of 166 pC/N at 80% BTO weight percentage. To evaluate the properties of 3D printed BTO ceramics, a 1.4 MHz focused ultrasonic array was fabricated and characterized. The -6dB bandwidth of the array was 40%, and the insertion loss at the center frequency was 50 dB. The results show that the printed BTO ceramics array have good potential to be used in ultrasonic transducers for various applications.

Intravascular confocal photoacoustic endoscope with dual-element ultrasonic transducer.

We have developed an intravascular confocal photoacoustic (PA) endoscope with symmetrically aligned dual-element ultrasonic transducers. By combining focused laser excitation and focused acoustic collection, the intravascular confocal PA endoscope is capable of realizing resolution enhanced intravascular PA imaging with improved signal-to-noise ratio (SNR) to ameliorate the resolution reduction caused by laser scattering with increasing tissue depth. The detection sensitivity of the endoscope is improved by 5 dB compared with that of single transducer endoscope, and the transverse resolution of the system can up to 13 µm. Intravascular PA tomography of a normal vessel and an atherosclerotic vessel have been performed to demonstrate the imaging ability of the system. This intravascular confocal PA endoscope with an outer diameter of 1.2 mm supports potential for clinical applications in intravascular plaque imaging and subsequent diagnosis.

Cost-efficient laser-diode-induced optical-resolution photoacoustic microscopy for two-dimensional/three-dimensional biomedical imaging.

Solid-state laser systems, such as traditional Nd:YAG-based lasers, are commonly used for noninvasive biomedical photoacoustics with nanosecond pulse duration and millijoule pulse energy. However, such lasers are both bulky and expensive for use as a handy tool for clinical applications. As an alternative, a semiconductor light source has the advantages of being compact, inexpensive, and robust. In addition, the main drawback of low peak output power may make it exactly suitable for the imaging modalities, which require relatively low pulse energies, such as acoustic- and optical-resolution photoacoustic microscopy (AR/OR-PAM). We propose a cost-efficient OR-PAM for two-dimensional/three-dimensional (2-D/3-D) biological imaging based on a pulsed near-infrared laser diode. By raster scanning, typical 2-D photoacoustic images were obtained at different scales, and 3-D surface renderings were clearly reconstructed with a marching cubes algorithm. This initial study would promote the production of portable OR-PAM technology for clinical and biomedical applications.

Characterization of lipid-rich aortic plaques by intravascular photoacoustic tomography: ex vivo and in vivo validation in a rabbit atherosclerosis model with histologic correlation.

BACKGROUND: Histologic studies have demonstrated that lipid content and its spatial distribution is related to plaque vulnerability. However, in vivo imaging is still limited. Photoacoustic imaging may provide novel in vivo insights into these lipid-rich plaques. OBJECTIVES: This study sought to examine whether intravascular photoacoustic tomography (IVPAT) allows localization and quantification of lipid content in atherosclerotic plaques. METHODS: Rabbits fed with a high-fat/high-cholesterol diet served as the atherosclerotic model. Catheter-based IVPAT was used to evaluate pixel-based lipid relative concentration (LRC) of the vessel wall. The aorta of 4 groups of rabbits (n = 12) were examined ex vivo with IVPAT after 0, 5, 10, and 15 weeks of a high-fat diet, respectively. Six rabbits underwent 3-dimensional (3D) IVPAT after 20 weeks of the high-fat diet. Three rabbits were examined in vivo using IVPAT without interruption of blood flow. Concentration-based lipid map and quantitative index were calculated. For subsequent histologic correlation, all specimens were evaluated with Oil Red O staining. RESULTS: Cross-sectional LRC maps allowed visualization of concentration and depth information of lipid content in the atherosclerotic plaques. Lipid accumulation within plaque, assessed by the maximum LRC, mean LRC, and high lipid content area correlated to duration of a high-fat diet. Three-dimensional LRC maps enabled overall evaluation of focal plaques in an intact explanted aorta including spatial and structural features. In vivo-obtained LRC maps accurately showed the structure of lipid core with high contrast. Ex vivo and in vivo IVPAT results were highly consistent with histological results. CONCLUSIONS: In an animal model, IVPAT allowed characterization of spatial and quantitative features of lipid-rich plaques.

Noninvasive photoacoustic detecting intraocular foreign bodies with an annular transducer array.

We present a fast photoacoustic imaging system based on an annular transducer array for detection of intraocular foreign bodies. An eight-channel data acquisition system is applied to capture the photoacoustic signals using multiplexing and the total time of data acquisition and transferring is within 3 s. A limited-view filtered back projection algorithm is used to reconstruct the photoacoustic images. Experimental models of intraocular metal and glass foreign bodies were constructed on ex vivo pig's eyes and clear photoacoustic images of intraocular foreign bodies were obtained. Experimental results demonstrate the photoacoustic imaging system holds the potential for in clinic detecting the intraocular foreign bodies.