FOXK1 promotes cell growth through activating wnt/ß-catenin pathway and emerges as a novel target of miR-137 in glioma.

Glioma is the most common primary malignant brain tumor in adults. Forkhead box k1 (FOXK1) was reported to be dysregulated and play important roles in multiple human cancers. However, the expression pattern and roles of FOXK1 in glioma has never been investigated. In this study, we firstly observed that the expression of FOXK1 was significantly increased in glioma tissue samples and cell lines. Functional assays demonstrated that overexpression of FOXK1 promoted proliferation, cell cycle transition and inhibited apoptosis in glioma cell lines. On the contrary, knockdown of FOXK1 exhibited an opposite effect on glioma cells proliferation, cell cycle and apoptosis. Data of western blot indicated that FOXK1 overexpression increased while FOXK1 knockdown decreased the levels of ß-catenin, c-myc and cyclinD1 in glioma cells. Moreover, we demonstrated that FOXK1 was a novel target of miR-137 in glioma and FOXK1 restoration abolished the tumor suppressive effect of miR-137 in glioma cells. Statistical analysis showed that the mRNA level of FOXK1 was inversely correlated with miR-137 expression in glioma tissues. In conclusion, the present study demonstrated that FOXK1 promoted cell growth through activating wnt/ß-catenin pathway and is negatively regulated by miR-137 in glioma.

Statistics of extremes in oil spill risk analysis.

The Deepwater Horizon oil spill (DWH) in 2010 in the Gulf of Mexico is the largest accidental marine oil spill in the history of the petroleum industry. After DWH, key questions were asked: What is the likelihood that a similar catastrophic oil spill (with a volume over 1 million barrels) will happen again? Is DWH an extreme event or will it happen frequently in the future? The extreme value theory (EVT) has been widely used in studying rare events, including damage from hurricanes, stock market crashes, insurance claims, flooding, and earthquakes. In this paper, the EVT is applied to analyze oil spills in the U.S. outer continental shelf (OCS). Incorporating the 49 years (1964-2012) of OCS oil spill data, the EVT is capable of describing the oil spills reasonably well. The return period of a catastrophic oil spill in OCS areas is estimated to be 165 years, with a 95% confidence interval between 41 years and more than 500 years. Sensitivity tests indicate that the EVT results are relatively stable. The results of this study are very useful for oil spill risk assessment, contingency planning, and environmental impact statements on oil exploration, development, and production.

Three dimensional water quality modeling of a shallow subtropical estuary.

Knowledge of estuarine hydrodynamics and water quality comes mostly from studies of large estuarine systems. The processes affecting algae, nutrients, and dissolved oxygen (DO) in small and shallow subtropical estuaries are relatively less studied. This paper documents the development, calibration, and verification of a three dimensional (3D) water quality model for the St. Lucie Estuary (SLE), a small and shallow estuary located on the east coast of south Florida. The water quality model is calibrated and verified using two years of measured data. Statistical analyses indicate that the model is capable of reproducing key water quality characteristics of the estuary within an acceptable range of accuracy. The calibrated model is further applied to study hydrodynamic and eutrophication processes in the estuary. Modeling results reveal that high algae concentrations in the estuary are likely caused by excessive nutrient and algae supplies in freshwater inflows. While algal blooms may lead to reduced DO concentrations near the bottom of the waterbody, this study indicates that stratification and circulation induced by freshwater inflows may also contribute significantly to bottom water hypoxia in the estuary. It is also found that high freshwater inflows from one of the tributaries can change the circulation pattern and nutrient loading, thereby impacting water quality conditions of the entire estuary. Restoration plans for the SLE ecosystem need to consider both a reduction of nutrient loading and regulation of the freshwater discharge pattern.

Gene expression profiles of human osteosarcoma cell sublines with different pulmonary metastatic potentials.

AIM: to screen the pulmonary metastasis-associated molecules of Osteosarcoma and evaluate their functions concerning prognosis prediction. METHODS: cDNA microarray analysis has been applied to 2 pairs of osteosarcoma cell sublines with differential metastatic potentials to the lung. Immunohistochemistry and survival analysis have been performed to clinical samples of osteosarcoma patients. RESULT: Analysis detected 484 differentially expressed genes between the high metastatic subline, F5M2, and the low metastatic subline, F4. There were 1257 genes differentially expressed between newly established high-metastatic sublines named Saos-2M2 and its parental cell line Saos-2. Furthermore, 16 commonly up-regulated genes and 5 commonly down-regulated genes were identified by clustering analysis. EREG and CHST2, two genes not previously described in osteosarcoma, were finally seen to be differentially expressed in all examined osteosarcoma cell lines and in samples between the different prognosis sample groups. Survival analysis also confirmed these two molecules could be used to predict the outcome of OSA patients. CONCLUSION: This work represents a rationale approach to the evaluation of microarray data and will be useful to identify genes that may be causally associated with metastasis. EREG and CHST2 will be likely considered as clinical molecular markers to predict the outcome of OSA.

Use of physical sciences in support of environmental management.

Offshore drilling for oil and gas has been conducted since the early 1900s. Oil and gas under the seabed continue to be an important part of the energy resources of the United States. The need to balance the value of these resources against the potential for environmental damage is an important concern. This article explains why and how the Minerals Management Service (MMS) of the US Department of the Interior uses research in physical sciences to help fulfill its environmental goals, and it provides background information on the role of physical sciences in decision-making for Outer Continental Shelf (OCS) oil, gas, and other minerals development. Established in the 1970s, the MMS' Environmental Studies Program is a highly focused marine research program designed to provide the environmental information necessary for OCS energy and nonenergy minerals planning and development activities. The physical sciences research supported by MMS includes physical oceanography, oil-spill risk analyses, atmospheric sciences, and sand and gravel studies. Instead of giving a comprehensive review on physical sciences research in MMS, this article presents sample MMS studies and illustrates how these studies are utilized to support decision-making in environmental management.