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1.
Sci Rep ; 12(1): 11119, 2022 07 01.
Article in English | MEDLINE | ID: mdl-35778441

ABSTRACT

With the increase in extremely low birth weight (ELBW) infants, their outcome attracted worldwide attention. However, in China, the related studies are rare. The hospitalized records of ELBW infants discharged from twenty-six neonatal intensive care units in Guangdong Province of China during 2008-2017 were analyzed. A total of 2575 ELBW infants were enrolled and the overall survival rate was 55.11%. From 2008 to 2017, the number of ELBW infants increased rapidly from 91 to 466, and the survival rate improved steadily from 41.76% to 62.02%. Increased survival is closely related to birth weight (BW), regional economic development, and specialized hospital. The incidence of complications was neonatal respiratory distress syndrome (85.2%), oxygen dependency at 28 days (63.7%), retinopathy of prematurity (39.3%), intraventricular hemorrhage (29.4%), necrotizing enterocolitis (12.0%), and periventricular leukomalacia (8.0%). Among the 1156 nonsurvivors, 90.0% of infants died during the neonatal period (≤ 28 days). A total of 768 ELBW infants died after treatment withdrawal, for reasons of economic and/or poor outcome. The number of ELBW infants is increasing in Guangdong Province of China, and the overall survival rate is improving steadily.


Subject(s)
Enterocolitis, Necrotizing , Infant, Premature, Diseases , Cohort Studies , Enterocolitis, Necrotizing/epidemiology , Humans , Infant , Infant Mortality , Infant, Extremely Low Birth Weight , Infant, Newborn , Infant, Premature, Diseases/epidemiology
2.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(5): 514-520, 2022 May 15.
Article in Chinese | MEDLINE | ID: mdl-35644191

ABSTRACT

OBJECTIVES: To study the effect of sex on the clinical outcome of extremely preterm infants (EPIs)/extremely low birth weight infants (ELBWIs) by propensity score matching. METHODS: A retrospective analysis was performed for the medical data of 731 EPIs or ELBWIs who were admitted from January 1, 2011 to December 31, 2020. These infants were divided into two groups: male and female. A propensity score matching analysis was performed at a ratio of 1:1. The matching variables included gestational age, birth weight, percentage of withdrawal from active treatment, percentage of small-for-gestational-age infant, percentage of use of pulmonary surfactant, percentage of 1-minute Apgar score ≤3, percentage of mechanical ventilation, duration of mechanical ventilation, percentage of antenatal use of inadequate glucocorticoids, and percentage of hypertensive disorders in pregnancy. The two groups were compared in the incidence rate of main complications during hospitalization and the rate of survival at discharge. RESULTS: Before matching, compared with the female group, the male group had significantly higher incidence rates of neonatal respiratory distress syndrome, bronchopulmonary dysplasia (BPD), severe intraventricular hemorrhage, periventricular leukomalacia, necrotizing enterocolitis, and patent ductus arteriosus (P<0.05), while after matching, the male group only had a significantly higher incidence rate of BPD than the female group (P<0.05). There was no significant difference in the rate of survival at discharge between the two groups before and after matching (P>0.05). CONCLUSIONS: Male EPIs/ELBWIs have a higher risk of BPD than female EPIs/ELBWIs, but male and female EPIs/ELBWIs tend to have similar outcomes.


Subject(s)
Bronchopulmonary Dysplasia , Infant, Extremely Low Birth Weight , Bronchopulmonary Dysplasia/epidemiology , Bronchopulmonary Dysplasia/etiology , Female , Humans , Infant , Infant, Extremely Premature , Infant, Newborn , Male , Pregnancy , Propensity Score , Retrospective Studies , Sex Characteristics
3.
Front Cell Dev Biol ; 9: 637248, 2021.
Article in English | MEDLINE | ID: mdl-33842462

ABSTRACT

Premature infants have a high risk of bronchopulmonary dysplasia (BPD), which is characterized by abnormal development of alveoli and pulmonary vessels. Exosomes and exosomal miRNAs (EXO-miRNAs) from bronchoalveolar lavage fluid are involved in the development of BPD and might serve as predictive biomarkers for BPD. However, the roles of exosomes and EXO-miRNAs from umbilical cord blood of BPD infants in regulating angiogenesis are yet to be elucidated. In this study, we showed that umbilical cord blood-derived exosomes from BPD infants impaired angiogenesis in vitro. Next-generation sequencing of EXO-miRNAs from preterm infants without (NBPD group) or with BPD (BPD group) uncovered a total of 418 differentially expressed (DE) EXO-miRNAs. These DE EXO-miRNAs were primarily enriched in cellular function-associated pathways including the PI3K/Akt and angiogenesis-related signaling pathways. Among those EXO-miRNAs which are associated with PI3K/Akt and angiogenesis-related signaling pathways, BPD reduced the expression of hsa-miR-103a-3p and hsa-miR-185-5p exhibiting the most significant reduction (14.3% and 23.1% of NBPD group, respectively); BPD increased hsa-miR-200a-3p expression by 2.64 folds of the NBPD group. Furthermore, overexpression of hsa-miR-103a-3p and hsa-miR-185-5p in normal human umbilical vein endothelial cells (HUVECs) significantly enhanced endothelial cell proliferation, tube formation, and cell migration, whereas overexpressing hsa-miR-200a-3p inhibited these cellular responses. This study demonstrates that exosomes derived from umbilical cord blood of BPD infants impair angiogenesis, possibly via DE EXO-miRNAs, which might contribute to the development of BPD.

4.
Nan Fang Yi Ke Da Xue Xue Bao ; 36(7): 952-6, 2016 Jun 20.
Article in Chinese | MEDLINE | ID: mdl-27435775

ABSTRACT

OBJECTIVE: To investigate the effect of docosahexaenoic acid (DHA) on invasiveness of aflatoxin B1 (AFB1)-induced hepatocellular carcinoma cells in vitro. METHODS: HepG2.2.15 cells were exposed to different concentrations of AFB1 and DHA plus AFB1. The cell migration and invasion were assessed using wound-healing and Transwell assay, and flow cytometry was used to analyze the cell cycle changes. The ultrastructural changes of the cells were observed by transmission electron microscopy. RESULTS: Compared with the control group, the cells exposed to2 µmol/L AFB1 showed obviously enhanced migration and invasion with decreased cell ratio in G1/G1 phase and increased cell ratio in G2/M phase but no changes in S phase cells; transmission electron microscopy revealed the presence of multiple nucleoli and significantly increased mitochondria and Golgi apparatus in the exposed cells. Compared with AFB1-exposed cells, the cells treated with DHA and AFB1 showed decreased migration and invasion abilities, and the G1/G1 phase cells increased and G2/M phase cells decreased significantly; ultrastructurally, the cells contained single nucleoli with decreased mitochondria and vacuolization occurred in the cytoplasm. CONCLUSION: DHA can significantly inhibit AFB1-induced enhancement of cell migration and invasion in hepatocellular carcinoma cells in vitro.


Subject(s)
Aflatoxin B1/pharmacology , Carcinoma, Hepatocellular/pathology , Cell Movement/drug effects , Docosahexaenoic Acids/pharmacology , Liver Neoplasms/pathology , Cell Cycle , Golgi Apparatus , Hep G2 Cells , Humans , Mitochondria , Neoplasm Invasiveness
5.
Zhong Yao Cai ; 39(5): 943-7, 2016 May.
Article in Chinese | MEDLINE | ID: mdl-30132625

ABSTRACT

Objective: To establish a principal component analysis( PCA) method for selecting Litsea mollis,to determine the selection criteria, in order to provide a theoretical basis for the early excellenting, selective breeding of Litsea mollis. Methods: The seedling origin of Litsea mollis plantion in Wanzhou district of Chongqing province were used as the research object,72 plus trees were selected by using 5 dominant comparative method. Its growth and economic traits were observed and analyzed by variance analysis and PCA method. Results: The variatice analysis result showed that the traits existed rich genetic differences. The PCA analysis result showed that in rotated component matrix, tree height, crown area, east-west crown, north-south crown, diameter at breast height were used as the selection optimal criteria of Litsea mollis. Four plants with superior integrated economical were selected, which namely Y12-4,Y12,Y11-3and Y10. Conclusion: The rich variability of Litsea mollis provide the prerequisite condition for plus tree selection. The results conform to the phenotypic and indicate that the optimization methods are scientific and feasible.


Subject(s)
Litsea , Trees , Phenotype , Principal Component Analysis , Seedlings
6.
Ann Rheum Dis ; 73(9): 1719-27, 2014 Sep.
Article in English | MEDLINE | ID: mdl-23852692

ABSTRACT

BACKGROUND: An exogenous supplement of n-3 polyunsaturated fatty acids (PUFAs) has been reported to prevent osteoarthritis (OA) through undefined mechanisms. OBJECTIVE: This study investigated the effect of alterations in the composition of endogenous PUFAs on OA, and associations of PUFAs with mammalian target of rapamycin complex 1 (mTORC1) signalling, a critical autophagy pathway in fat-1 transgenic (TG) mice. METHODS: fat-1 TG and wild-type mice were used to create an OA model by resecting the medial meniscus. The composition of the endogenous PUFAs in mouse tissues was analysed by gas chromatography, and the incidence of OA was evaluated by micro-computed tomography (micro-CT), scanning electron microscopy and histological methods. Additionally, primary chondrocytes were isolated and cultured. The effect of exogenous and endogenous PUFAs on mTORC1 activity and autophagy in chondrocytes was assessed. RESULTS: The composition of endogenous PUFAs of TG mice was optimised both by increased n-3 PUFAs and decreased n-6 PUFAs, which significantly alleviated the articular cartilage destruction and osteophytosis in the OA model (p<0.01), decreased protein expression of matrix metalloproteinase-13 (MMP-13) and ADAMTS-5 (a disintegrin and metalloproteinase with thrombospondin motifs) in the articular cartilage (p<0.01) and reduced chondrocyte number and loss of cartilage extracellular matrix. Both exogenous and endogenous n-3 PUFAs downregulated mTORC1 activity and promoted autophagy in articular chondrocytes. Conversely, mTORC1 pathway activation suppressed autophagy in articular chondrocytes. CONCLUSIONS: Enhancement of the synthesis of endogenous n-3 PUFAs from n-6 PUFAs can delay the incidence of OA, probably through inhibition of mTORC1, promotion of autophagy and cell survival in cartilage chondrocytes. Future investigation into the role of the endogenous n-6/n-3 PUFAs composition in OA prevention and treatment is warranted.


Subject(s)
Arthritis, Experimental/prevention & control , Fatty Acids, Omega-3/biosynthesis , Multiprotein Complexes/physiology , Osteoarthritis/prevention & control , TOR Serine-Threonine Kinases/physiology , ADAM Proteins/metabolism , ADAMTS5 Protein , Animals , Arthritis, Experimental/etiology , Arthritis, Experimental/pathology , Autophagy/physiology , Cadherins/genetics , Cartilage, Articular/metabolism , Cartilage, Articular/ultrastructure , Chondrocytes/pathology , Disease Progression , Fatty Acids, Omega-3/physiology , Fatty Acids, Omega-6/biosynthesis , Female , Matrix Metalloproteinase 13/metabolism , Mechanistic Target of Rapamycin Complex 1 , Mice , Mice, Inbred C57BL , Mice, Transgenic , Microscopy, Electron, Scanning , Osteoarthritis/etiology , Osteoarthritis/pathology , Proteoglycans/metabolism , Signal Transduction/physiology
7.
Nan Fang Yi Ke Da Xue Xue Bao ; 31(9): 1504-8, 2011 Sep.
Article in Chinese | MEDLINE | ID: mdl-21945753

ABSTRACT

OBJECTIVE: To evaluate the effect of metformin on the apoptosis of renal cell carcinoma (RCC) cells in vitro and its mechanisms. METHODS: Fluorescent microscopy and flow cytometry were used to examine the changes in the apoptosis of 786-O cells after metformin treatment. The possible signaling molecules involved in this process were analyzed by immunoblot analysis of AMP-activated protein kinase (AMPK) signaling and caspase 9. RESULTS: Metformin induced apoptosis and caspase 9 activation in 786-O cells in low-serum medium but not in normal-serum medium. Metformin also induced AMPK activation in 786-O cells, but this activation was not associated with the cell proliferation inhibition or apoptosis-inducing effect of metformin. CONCLUSION: Metformin can induce apoptosis of RCC cells in vitro, suggesting its potential as a therapeutic agent for RCC.


Subject(s)
Carcinoma, Renal Cell/pathology , Kidney Neoplasms/pathology , Metformin/pharmacology , Apoptosis/drug effects , Caspase 9/metabolism , Cell Line, Tumor , Cell Proliferation/drug effects , Humans
8.
Anat Rec (Hoboken) ; 294(8): 1337-43, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21717584

ABSTRACT

It has been reported that metformin, a biguanide derivative widely used in type II diabetic patients, has antitumor activities in some cancers by activation of AMP-activated protein kinase (AMPK). But its role in nasopharyngeal carcinoma (NPC) is not known. Here, we reported for the first time that 1-50 mM of metformin in a dose- and time-dependent manner suppressed cell proliferation and colony formation in NPC cell line, C666-1. Further studies revealed that the protein level of cyclin D1 decreased and the percentage of the cells in G0/G1 phase increased by 5 mM metformin treatment. Metformin also induced the phosphorylation of AMPK (T172) in a time-dependent manner. Mammalian target of rapamycin complex 1 (mTORC1), which is negatively regulated by AMPK and plays a central role in cell growth and proliferation, was inhibited by metformin, as manifested by dephosphorylation of its downstream targets 40S ribosomal S6 kinase 1 (S6K1) (T389), the eukaryotic translation initiation factor 4E (eIF4E)-binding protein 1 (4E-BP1) (T37/46) and S6 (S235/236) in C666-1 cells. In a summary, metformin prevents proliferation of C666-1 cells by down-regulating cyclin D1 level and inducing G1 cell cycle arrest. AMPK-mediated inhibition of mTORC1 signaling may be involved in this process.


Subject(s)
Antineoplastic Agents/pharmacology , Cell Proliferation/drug effects , G1 Phase Cell Cycle Checkpoints/drug effects , Metformin/pharmacology , Nasopharyngeal Neoplasms/pathology , AMP-Activated Protein Kinases/metabolism , Adaptor Proteins, Signal Transducing/metabolism , Carcinoma , Cell Cycle Proteins , Cell Line, Tumor , Cyclin D1/metabolism , Dose-Response Relationship, Drug , Humans , Mechanistic Target of Rapamycin Complex 1 , Multiprotein Complexes , Nasopharyngeal Carcinoma , Nasopharyngeal Neoplasms/metabolism , Phosphoproteins/metabolism , Phosphorylation , Proteins/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Ribosomal Protein S6 Kinases, 70-kDa/metabolism , Signal Transduction/drug effects , TOR Serine-Threonine Kinases , Time Factors
9.
J Cell Biochem ; 112(10): 2902-9, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21618594

ABSTRACT

Anti-diabetic drug metformin has been shown to enhance osteoblasts differentiation and inhibit osteoclast differentiation in vitro and prevent bone loss in ovariectomized (OVX) rats. But the mechanisms through which metformin regulates osteoclastogensis are not known. Osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL) are cytokines predominantly secreted by osteoblasts and play critical roles in the differentiation and function of osteoclasts. In this study, we demonstrated that metformin dose-dependently stimulated OPG and reduced RANKL mRNA and protein expression in mouse calvarial osteoblasts and osteoblastic cell line MC3T3-E1. Inhibition of AMP-activated protein kinase (AMPK) and CaM kinase kinase (CaMKK), two targets of metformin, suppressed endogenous and metformin-induced OPG secretion in osteoblasts. Moreover, supernatant of osteoblasts treated with metformin reduced formation of tartrate resistant acid phosphatase (TRAP)-positive multi-nucleated cells in Raw264.7 cells. Most importantly, metformin significantly increased total body bone mineral density, prevented bone loss and decreased TRAP-positive cells in OVX rats proximal tibiae, accompanied with an increase of OPG and decrease of RANKL expression. These in vivo and in vitro studies suggest that metformin reduces RANKL and stimulates OPG expression in osteoblasts, further inhibits osteoclast differentiation and prevents bone loss in OVX rats.


Subject(s)
Metformin/pharmacology , Osteoblasts/drug effects , Osteoblasts/metabolism , Osteoprotegerin/metabolism , RANK Ligand/metabolism , Animals , Blotting, Western , Cell Differentiation/drug effects , Cells, Cultured , Enzyme-Linked Immunosorbent Assay , Female , Mice , Osteoporosis/prevention & control , Ovariectomy , RANK Ligand/genetics , Rats , Rats, Sprague-Dawley , Real-Time Polymerase Chain Reaction , Signal Transduction/drug effects , Skull/cytology , Tibia/metabolism
10.
Mol Pharm ; 8(2): 488-97, 2011 Apr 04.
Article in English | MEDLINE | ID: mdl-21271712

ABSTRACT

Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) the etiology of which has not yet been fully clarified. Cytokine interleukin-10 (IL-10) plays a central role in downregulating inflammatory cascade in UC and is likely a candidate for therapeutic intervention. However, its intravenous administration is costly and inconvenient. Therefore, we established a novel IL-10 delivery system by transforming a hIL-10-containing plasmid into B. longum (BL-hIL-10) and investigated its effects on 5% dextran sulfate sodium (DSS)-induced ulcerative colitis in mice and the possible underlying mechanism. Our results show that (1) hIL-10 was expressed and secreted into the culture supernatant of BL-hIL-10 after L-arabinose induction in vitro as examined by Western blot, enzyme-linked immunosorbent assay (ELISA) and RT-PCR; (2) addition of BL-hIL-10 culture supernatant had no cytotoxic effect and morphological alteration, but significantly inhibited the enhancement of proinflammatory cytokines by lipopolysaccharide (LPS) in THP-1 cells; (3) oral administration of BL-hIL-10 alleviated colitis syndrome of the model mice, attenuated colitis-activated NF-κB pathway measured by DNA-binding assay and colitis-elevated expression of proinflammatory cytokines examined with CCK cytotoxic kits, and upregulated CD4+CD25+Foxp3+ Treg in blood and mesenteric lymph nodes measured by flow cytometry. In conclusion, BL-hIL-10 as a novel oral hIL-10 delivery system has been successfully established and oral administration of BL-hIL-10 alleviated inflammatory damage of colonic tissue in the model mice by blocking the colitis-activated NF-κB pathway and upregulating CD4+CD25+Foxp3+ Treg in blood and mesenteric lymph nodes in mice.


Subject(s)
Bifidobacterium/metabolism , Colitis/drug therapy , Dextran Sulfate/toxicity , Drug Carriers , Interleukin-10/administration & dosage , Interleukin-10/metabolism , Administration, Oral , Animals , Bifidobacterium/genetics , Blotting, Western , Colitis/chemically induced , Colitis/metabolism , Cytokines/metabolism , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Lipopolysaccharides/pharmacology , Male , Mice , Mice, Inbred BALB C , Monocytes/drug effects , Monocytes/metabolism , NF-kappa B/genetics , NF-kappa B/metabolism , Peroxidase/metabolism , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction , T-Lymphocytes, Regulatory/immunology , T-Lymphocytes, Regulatory/metabolism
11.
Planta Med ; 77(5): 421-7, 2011 Mar.
Article in English | MEDLINE | ID: mdl-20979017

ABSTRACT

Nuclear factor- κB (NF- κB) plays a pivotal role in the regulation of immune and inflammatory responses. The real-time expression level of NF- κB reflects the development of ulcerative colitis (UC). Polydatin has vast pharmacological activities, including inhibiting the production of inflammatory mediators, inducing the production of antioxidants, regulating immune function, etc. The purpose of this study was to investigate the potential inhibitory effects of polydatin on NF- κB pathway activation in a mouse UC model. The results showed that polydatin treatment downregulated NF- κB p65 activity and expression, blocked the expression of TNF- α, IL-6 and IL-1 ß at both mRNA and protein levels, decreased myeloperoxidase (MPO) activity, and alleviated inflammatory damage of colitis in mice with UC (p < 0.05), suggesting that the anti-inflammation effects of polydatin can be attributed, at least partially, to the blocking of the NF- κB pathway.


Subject(s)
Anti-Inflammatory Agents/therapeutic use , Colitis, Ulcerative/drug therapy , Glucosides/therapeutic use , NF-kappa B/antagonists & inhibitors , Stilbenes/therapeutic use , Animals , Anti-Inflammatory Agents/chemistry , Colitis, Ulcerative/chemically induced , Colitis, Ulcerative/metabolism , Dextran Sulfate/toxicity , Disease Models, Animal , Glucosides/chemistry , Inflammation Mediators/metabolism , Interleukin-1beta/antagonists & inhibitors , Interleukin-1beta/genetics , Interleukin-1beta/metabolism , Interleukin-6/antagonists & inhibitors , Interleukin-6/genetics , Interleukin-6/metabolism , Male , Mice , Mice, Inbred BALB C , NF-kappa B/genetics , NF-kappa B/metabolism , Peroxidase/antagonists & inhibitors , Peroxidase/metabolism , Pilot Projects , RNA, Messenger/metabolism , Resveratrol , Signal Transduction/drug effects , Stilbenes/chemistry , Stilbenes/pharmacology , Tumor Necrosis Factor-alpha/antagonists & inhibitors , Tumor Necrosis Factor-alpha/genetics , Tumor Necrosis Factor-alpha/metabolism
12.
Arch Med Res ; 41(4): 288-94, 2010 May.
Article in English | MEDLINE | ID: mdl-20637373

ABSTRACT

BACKGROUND AND AIMS: Oxidant/antioxidant balance is suggested to be an important factor for the recurrence and progression of ulcerative colitis (UC). The aim of the study is to investigate the potential protective role of resveratrol (Res) against dextran sodium sulfate (DSS)-induced oxidative damage in colon of mice with UC. METHODS: UC was induced in mice by oral administration of synthetic DSS (molecular weight 5000) for 7 days. Mice were divided into normal group, colitis control group, low-dose Res-treated group (RLD-treated group), and high-dose Res-treated group (RHD-treated group). Inhibitory effects of concomitant treatment with Res were assessed daily using a Disease Activity Index (DAI) and severity of histological changes. MDA, MPO, SOD and GSH-PX activity of colonic tissue were determined in colon samples by chemical colorimetry. TNF-alpha, IL-8, IFN-gamma, p22(phox) and gp91(phox) expression levels were detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR), ELISA, and Western blot analysis. RESULT: Administration of Res significantly inhibited the severity of UC compared to the colitis control group. Colonic tissue MDA and MPO activities decreased significantly in Res-treated groups compared to colitis control groups. Furthermore, colonic tissue SOD and GSH-Px activities increased significantly in Res-treated groups compared to colitis control groups. The expression levels of TNF-alpha, IL-8, IFN-gamma, p22(phox), and gp91(phox) also decreased significantly in the Res-treated group compared to the colitis control group. CONCLUSIONS: Oral administration of Res exerts marked inhibitory effects on UC in mice. Resveratrol may play an important role in preventing DSS-induced oxidative damage.


Subject(s)
Antioxidants/pharmacology , Colitis, Ulcerative/metabolism , Dextran Sulfate/toxicity , Stilbenes/pharmacology , Animals , Base Sequence , Blotting, Western , Colitis, Ulcerative/chemically induced , Colitis, Ulcerative/enzymology , Colon/enzymology , Colon/metabolism , DNA Primers , Enzyme-Linked Immunosorbent Assay , Glutathione Peroxidase/metabolism , Male , Malondialdehyde/metabolism , Mice , Mice, Inbred BALB C , Peroxidase/metabolism , Reactive Oxygen Species/metabolism , Resveratrol , Reverse Transcriptase Polymerase Chain Reaction
13.
Nan Fang Yi Ke Da Xue Xue Bao ; 30(6): 1226-9, 2010 Jun.
Article in Chinese | MEDLINE | ID: mdl-20584639

ABSTRACT

OBJECTIVE: To obtain recombinant N-and C-terminal of FKBP38 and prepare anti-FKBP38 polyclonal antibody for Western blotting (WB), immunohistochemical (IHC) and immunofluorescence (IF) analyses. METHODS: The N-terminal (1-207 aa) and C-terminal (209-387 aa) cDNA of FKBP38 were sub-cloned from the full-length cDNA of FKBP38 and ligated to prokaryotic expression plasmid pGEX-6P-1 for construction of the recombinant vectors pGEX-6P-1-FKBP38-N and pGEX-6P-1-FKBP38-C. After sequencing, the recombinant vectors were transformed into E.coli BL21 and GST-tagged FKBP38-NT and FKBP38-CT were induced by IPTG. The proteins were purified by Glutathione affinity chromatography column and characterized by SDS-PAGE. Rabbits were immunized with the purified recombinant protein to prepare the antiserum, which were analyzed by WB, IHC and IF. RESULTS: The recombinant vectors pGEX-6P-1-FKBP38-N and pGEX-6P-1-FKBP38-C were successfully constructed. After IPTG induction, the E.coli transformed with these plasmids expressed GST-tagged protein, which was successfully purified. Western blotting demonstrated that the purified antibody could specifically bind to FKBP38 in various cell lines. Immunofluorescence assay showed that FKBP38 was located mainly on the mitochondria. Immunohistochemical analysis revealed cytoplasmic location of FKBP38 in breast cells. CONCLUSION: We successfully expressed and purified N- and C-terminal of FKBP38, and FKBP38 polyclonal antibody we prepared can specifically recognize FKBP38 in SB, IF and IHC assays, which facilitates further functional investigation of FKBP38.


Subject(s)
Antibodies, Monoclonal/biosynthesis , Genetic Vectors/genetics , Tacrolimus Binding Proteins/biosynthesis , Fluorescent Antibody Technique , Humans , Immunohistochemistry , Recombinant Proteins/biosynthesis , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Tacrolimus Binding Proteins/genetics , Tacrolimus Binding Proteins/immunology
14.
Bull Environ Contam Toxicol ; 84(2): 221-4, 2010 Feb.
Article in English | MEDLINE | ID: mdl-19960179

ABSTRACT

A specific, sensitive method was developed for the analysis of chlormequat in wheat and soil by high performance chromatography/mass spectrometry. The fortified recoveries of soil were from 75.08% to 96.55%, with RSD 3.34%-15.18%, the limit of detection of the analytical method was 0.05 ng at a signal-to-noise ratio of 3, and the limit of quantification was 0.05, 0.1, 0.5 mg/kg for soil, wheat plants and wheat grain, respectively. The degradation dynamics and final residues of chlormequat in Beijing and Changchun were investigated. The half-life of chlormequat in wheat plants were 3.15 days in Beijing and 4.56 days in Changchun, while the half-life in soil was 3.88 days in Beijing and 4.51 days in Changchun. The final residues of chlormequat in soil were not detectable, and the final residues of chlormequat in wheat grain were below 0.50 mg/kg except for 3.51 mg/kg from high dosage plot of Changchun. The fact that all the final residues were below 5 mg/kg (GB2763 in National standards of the People's Republic of China, maximum residue limits for pesticide in food, Beijing, 2005) suggested that chlormequat could be safely used in wheat crops with the suitable dosage and application.


Subject(s)
Chlormequat/analysis , Pesticide Residues/analysis , Plant Growth Regulators/analysis , Soil Pollutants/analysis , Soil/analysis , Triticum/metabolism , China , Chromatography, High Pressure Liquid , Flour/analysis , Half-Life , Mass Spectrometry , Reference Standards , Seeds/chemistry , Triticum/chemistry
15.
Neurosci Bull ; 24(1): 7-12, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18273070

ABSTRACT

OBJECTIVE: To investigate effects of K(ATP) opener on the expressions of caspase-12 mRNA and protein, and to explore the role of endoplasmic reticulum (ER) stress pathway in the mechanism of K(ATP) opener protecting against neuronal apoptosis after cerebral ischemia-reperfusion. METHODS: Two hundred rats were randomly divided into four groups: sham operation group, ischemia-reperfusion group, K(ATP) opener group, and K(ATP) blocker group. The middle cerebral artery occlusion (MCAO) model was established by intraluminal suture occlusion method; neuronal apoptosis was detected by TUNEL staining. The mRNA and protein expressions of caspase-12 were detected by semi-quantitative RT-PCR and immunohistochemical staining, respectively. RESULTS: In ischemia-reperfusion group, K(ATP) opener group and K(ATP) blocker group, the number of apoptotic cells and the mRNA and protein expressions of caspase-12 gradually increased following cerebral reperfusion, and reached the peak at 24 h. In K(ATP) opener group, the number of apoptotic cells was significantly less than that in ischemia-reperfusion group and K(ATP) blocker group at 12 h, 24 h, 48 h and 72 h (P< 0.05 or P< 0.01); while the mRNA and protein levels of caspase-12 were significantly less than those in ischemia-reperfusion group and K(ATP) blocker group at all times (P< 0.05 or P< 0.01). There were no differences between the ischemia-reperfusion group and K(ATP) blocker group at each time (P> 0.05). CONCLUSION: K(ATP) opener may protect neurons from apoptosis following the cerebral ischemia-reperfusion by inhibiting ER stress pathway.


Subject(s)
Brain Ischemia/metabolism , Caspase 12/metabolism , KATP Channels/agonists , Pinacidil/pharmacology , Reperfusion Injury/metabolism , Animals , Brain Ischemia/genetics , Caspase 12/genetics , Endoplasmic Reticulum Stress , Infarction, Middle Cerebral Artery/metabolism , Male , RNA, Messenger/metabolism , Rats , Rats, Wistar , Reperfusion Injury/genetics
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