ABSTRACT
Acute obstruction of coronary arteries leads to acute myocardial infarction (AMI), which causes unexpected death in humans. However, AMI cannot be easily detected in forensic examinations with traditional hematoxylin and eosin (H&E) staining. We analyzed whether cardiac troponin inhibitor (CTnI) could serve as a sensitive and specific early marker for diagnosing AMI in forensic medicine. We established an AMI model in rabbits by ligating the left ventricular branch and observed CTnI expression with immunohistochemistry after different ligation times. We found increased CTnI staining at the 0.5-h time point and depletion of CTnI staining with a 1-h ligation. The areas in which CTnI staining was depleted as seen with immunohistochemical analysis were consistent with the results of H&E staining. Next, human myocardium tissues from 30 persons who died from AMI and were subsequently examined in our forensic center were studied using immunohistochemistry with an antibody to human CTnI. Areas of infarction also showed depletion of CTnI staining. These findings suggested that immunohistochemical detection of CTnI is earlier, more sensitive, and myocardial tissue - specific as compared with H&E staining. CTnI may serve as an ideal marker for diagnosing AMI in forensic investigations.
Subject(s)
Myocardial Infarction/metabolism , Myocardial Ischemia/metabolism , Troponin/antagonists & inhibitors , Acute Disease , Animals , Biomarkers/metabolism , Disease Models, Animal , Humans , Immunohistochemistry/methods , Myocardial Infarction/diagnosis , RabbitsABSTRACT
OBJECTIVE: To estimate the application of prognosis evaluation of ulnar nerve injury by 1H-magnetic resonance spectroscopy (1H-MRS). METHODS: The metabolites of first dorsal interossei (FDI) of two hands from 12 healthy volunteers and 1 volunteer with complete ulnar nerve injury were detected by 1H-MRS and the data were statistically analyzed. RESULTS: For the FDI of healthy adults, the female peaks area of extra-myocellular lipids (EMCL) was higher than the male (P < 0.05); There was no significant difference in Cho, Cr and intra-myocellular lipids (IMCL) between male and female (P > 0.05); There was no significant difference in all the peaks area between the left and right hand (P > 0.05). The EMCL peak of the injury side was higher than that of the healthy side, and the area of FDI was reduced in the volunteer with ulnar nerve injury. CONCLUSION: Noninvasive and quantitative detection of 1H-MRS may be valuable for prognosis evaluation of peripheral nerve injury.
Subject(s)
Peripheral Nerve Injuries/diagnosis , Proton Magnetic Resonance Spectroscopy/methods , Ulnar Nerve/injuries , Adult , Female , Humans , Male , Prognosis , Sex Distribution , Ulnar Nerve/metabolismABSTRACT
OBJECTIVE: This study aimed to clarify the morphology of the Martin-Gruber anastomosis (MGA) in Chinese. METHODS: One hundred and five Chinese upper limbs (36 males and 20 femalese) were dissected to find the connections between medial nerve and ulnar nerve. The MGA was classified as previously described by Lee. RESULTS: MGA was found in 24 cases (22.9%), in 11 of the 36 male and 5 of the 20 female. There was no obvious difference in the frequency of MGA in both upper limbs. Most MGA ulnar position was located at the medial and distal segment of the forearm. CONCLUSION: MGA anatomy could play important role in forensic diagnosis of ulnar nerve injury in Chinese population.
Subject(s)
Median Nerve/abnormalities , Median Nerve/pathology , Nervous System Malformations/pathology , Ulnar Nerve , Cadaver , China/epidemiology , Expert Testimony/legislation & jurisprudence , Female , Humans , Male , Muscle, Skeletal/innervation , Nervous System Malformations/epidemiology , Nervous System Malformations/physiopathology , Ulnar Nerve/abnormalities , Ulnar Nerve/injuries , Ulnar Nerve/pathology , Upper Extremity/innervationABSTRACT
OBJECTIVE: To study the relationship between postmortem interval and the myofibril fragmentation index of skeletal muscle. METHODS: Rabbit skeletal muscle were left in the room temperature for different postmortem intervals, and the protein concentration of each sample was detected by using biuret method. Furthermore, the myofibril fragmentation index of each sample was measured under the protein concentration level of 0.5 mg/mL. RESULTS: The myofibril fragmentation index increased obviously according to the postmortem interval prolongation. CONCLUSION: The myofibril fragmentation index may be used on the estimation of early postmortem interval.
Subject(s)
Muscle, Skeletal/metabolism , Myofibrils/metabolism , Postmortem Changes , Proteins/metabolism , Animals , Calcium-Binding Proteins/metabolism , Female , Male , Rabbits , Spectrophotometry , Time FactorsABSTRACT
OBJECTIVE: To investigate the signification of CTnI in acute myocardial infarction. METHODS: The model of myocardial infarction was established by ligating the left ventricle branch. Immunohistochemistry and image analysis were used to detect the change of CTnI in the model, and The sensitivity of immunohistochemistry and HE coloration were also compared. RESULTS: The acute myocardial infarction tissue showed obvious depletion of CTnI, there was no characterization of myocardial infarction in HE coloration. CONCLUSION: CTnI is sensitive to diagnosis of acute myocardial infarction.
