ABSTRACT
OBJECTIVE: To study the clinical efficacy of lymphadenectomy of lung cancer by video-assisted thoracic small incision surgery. METHODS: Radical lobectomy or pneumonectomy and systemic lymphadenectomy were performed in 197 patients with NSCLC through video-assisted thoracoscopic surgery. Pathological type and grade, total number of dissected lymph nodes, number of metastatic lymph nodes, survival and life quality were evaluated. RESULTS: The average of dissected lymph nodes was 11.61 and 471 lymph nodes from 382 regions were confirmed to have metastasis. The metastatic rates were 24.1% and 20.6% respectively. There were no significant difference between video-assisted thoracic small incision surgery and conventional thoracotomy. The 1, 3, 5-year survival rates of stage I , II and III a had no significant difference between video-assisted thoracic small incision surgery and conventional thoracotomy. CONCLUSION: Video-assisted thoracic small incision surgery achieves a similar efficacy as the traditional lymphadenectomy and lung cancer radical resection.
Subject(s)
Carcinoma, Non-Small-Cell Lung/surgery , Lung Neoplasms/surgery , Thoracic Surgery, Video-Assisted , Adult , Aged , Carcinoma, Non-Small-Cell Lung/pathology , Female , Humans , Lung Neoplasms/pathology , Lymph Node Excision/methods , Male , Middle Aged , Neoplasm Staging , Treatment OutcomeABSTRACT
<p><b>UNLABELLED</b>To reduce the huge labor-cost in the screening in traditional monoclonal antibody generation, We established a new system for monoclonal antibody generation integrating with protein array. BALB/c mice were immunized by eight recombinant proteins respectively, and the positive hybridoma cells were obtained by cell fusion and ELISA screening. All the eight kinds of positive hybridoma cells were mixed, cloned, screened by protein array, and definite dilution cloned.</p><p><b>RESULTS</b>175 single cell clones were obtained by complex cloning, and 119 of those were positive clones. Then 8 positive cell lines were generated by the following 2 rounds definite dilution cloning. By comparing with the traditional method, we got 8 monoclonal antibodies using the combined protein array screening and multiplex cloning method in 1 cycle, and fewer amounts of antigens were used. As a result, the combined protein array and multiplex cloning method could be used as an economical, rapid and simple tool applying in high throughput monoclonal antibody generation.</p>
