TLR9 Exerts an Oncogenic Role in Promoting Osteosarcoma Progression Depending on the Regulation of NF-κB Signaling Pathway.

Osteosarcoma (OS) is the most common primary malignant bone tumor and is mainly diagnosed in children. Toll-like receptor 9 (TLR9) is expressed in various tumor cells and was correlated with cancer progression. However, the underlying mechanism of TLR9 on the OS progression remains unclear. Our previous study demonstrated that the expression of TLR9 was positively correlated with the development stage of OS. Herein, we further evaluated the actual roles and the molecular mechanism of TLR9 on regulating OS cell proliferation and metastasis. Our data showed that TLR9 was upregulated in OS cells compared to normal osteoblastic cells, and knockdown of TLR9 inhibited OS cell proliferation and induced cell cycle arrest by the decreased expression of cyclin D1, CDK2, and p-Rb, while TLR9 overexpression exerted the inverse effects. Furthermore, TLR9 overexpression could enhance the migration and invasion activities of the OS cells by the upregulation of matrix metalloproteinases 2 (MMP2) and MMP9, and the opposite result was observed in TLR9-silenced cells. Moreover, the nuclear factor kappa B (NF-κB) signaling pathway was activated by TLR9, and TLR9-induced malignant phenotype of OS cells was abrogated by the NF-κB antagonist BAY11-7082. Our study indicated that TLR9 might play a critical role in facilitating OS progression by activating the NF-κB signaling pathway, which may provide a valuable therapeutic target for OS.

Exploring multiple electrical layers overlying coal seams using the transient electromagnetic method.

The transient electromagnetic method (TEM) is widely applied in coal hydrogeological exploration owing to its sensitivity to low-resistivity bodies. However, when a coal seam is buried deep, particularly if there are multiple electrical layers in the vertical direction of the overlying stratum, the results of the calculation using the late-channel empirical formula of the TEM may no longer reflect the actual situation. In this study, we evaluated the principle of the one-dimensional (1D) Occam algorithm and the steps for performing an inversion. We proposed various two-, three-, and four-layer electrical models for inversion using the 1D Occam algorithm. Our results are consistent with the electrical distribution of the models, thus indicating the effectiveness of the algorithm. A test project of large-depth transient electromagnetic exploration in the Datong Coalfield in Shanxi, China, was selected for experimental verification. The 1D Occam inversion was used to successfully identify various electrical strata overlying the coal seam.