ABSTRACT
OBJECTIVE: To detect the correlation between the expression of topoisomerase 2 alpha (TOP2A) and the biological behaviors of human colorectal carcinoma. METHODS: Immunohistochemistry and real-time RT-PCR were used to detect the expression of TOP2A in colorectal carcinomas and normal mucosa. RESULTS: The protein and mRNA expressions of TOP2A in the metastatic lymph nodes were significantly higher than those in matched primary lesions and normal tissues (P<0.05). No significant difference was found in TOP2A expressions between normal mucosa and colorectal carcinomas. The protein and mRNA expressions of TOP2A were significantly correlated to the lymph node metastasis and invasion depth (P<0.05), but not to the differentiation of the tumor (P>0.05). CONCLUSION: TOP2A plays an important role in the invasion and metastasis of the colorectal carcinomas, and may serve as a valuable indicator for the diagnosis, treatment and the prognostic evaluation of the malignancy.
Subject(s)
Antigens, Neoplasm/metabolism , Colorectal Neoplasms/metabolism , Colorectal Neoplasms/pathology , DNA Topoisomerases, Type II/metabolism , DNA-Binding Proteins/metabolism , Female , Humans , Lymphatic Metastasis , Male , Poly-ADP-Ribose Binding ProteinsABSTRACT
OBJECTIVE: To develop a new stable and efficient reagent for methemoglobin reduction test. METHODS: The results of methemoglobin reduction test using the new reagent were compared with those by G6P/6PG ratio method and classic methemoglobin reduction test. RESULTS: The new reagent was stable for at least 6 months at room temperature and 12 months at 2-8 degrees celsius;. The results of the test using this new reagent were stable and reliable. CONCLUSION: The new reagent for methemoglobin reduction test allows easy operation with well reproducible results and can be used in clinical screening of glucose-6-phosphate dehydrogenase deficiency.
