ABSTRACT
<p><b>OBJECTIVE</b>To summarize the early diagnose and treatment approaches of Refractory Mycoplasma Pneumoniae Pneumonia (RMPP).</p><p><b>METHODS</b>Medical documents of 68 cases of RMPP were reviewed. Lab and radiology evident such as CBC, CRP, MP-IgM, X-ray, etc. were collected.</p><p><b>RESULTS</b>100% RMPP patients suffered from high fever. Positive sign of lung became clear with the development of the disease. Complications as impairment of liver function, cardiac function and rush developed in few patients. 2-4 rounds treatment of macrolides and Methyllprednisolone were necessary for RMPP while antibiotic may be considered when there were evidence of bacteria infection. Immunoglobulin was recommended to the patients when macrolides and steroid seemed ineffective. Bronchofibroscope played an active role regarding the diagnosis and treatment of RMPP.</p><p><b>CONCLUSION</b>Early diagnosis is crucial in RMPP. Combination of multitreatment approaches is the key to cure RMPP.</p>
Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Anti-Bacterial Agents , Therapeutic Uses , Macrolides , Therapeutic Uses , Mycoplasma pneumoniae , Virulence , Pneumonia, Mycoplasma , Diagnosis , Drug Therapy , Microbiology , Prednisolone , Therapeutic UsesABSTRACT
Plasmodium sporozoites suppress the respiratory burst and antigen presentation of Kupffer cells, which are regarded as the portal of invasion into hepatocytes. It is not known whether immune modulation of Kupffer cells can affect the liver stage. In the present study, we found that sporozoites inoculated into Wistar rats could be detected in the liver, spleen, and lung; however, most sporozoites were arrested in the liver. Sporozoites were captured by Kupffer cells lined with endothelial cells in the liver sinusoid before hepatocyte invasion. Pretreatment with TLR3 agonist poly(I:C) and TLR2 agonist BCG primarily activated Kupffer cells, inhibiting the sporozoite development into the exoerythrocytic form, whereas Kupffer cell antagonists dexamethasone and cyclophosphamide promoted development of the liver stage. Our data suggests that sporozoite development into its exoerythrocytic form may be associated with Kupffer cell functional status. Immune modulation of Kupffer cells could be a promising strategy to prevent malaria parasite infection.
Subject(s)
Immunologic Factors/pharmacology , Liver/parasitology , Plasmodium yoelii/growth & development , Adjuvants, Immunologic/pharmacology , Animals , Anopheles , BCG Vaccine/pharmacology , Cryoelectron Microscopy , Cyclophosphamide/pharmacology , Dexamethasone/pharmacology , Female , Glucocorticoids/pharmacology , Immunosuppressive Agents/pharmacology , Interferon Inducers/pharmacology , Kupffer Cells/parasitology , Liver/ultrastructure , Lung/parasitology , Male , Microscopy, Electron, Scanning , Plasmodium yoelii/drug effects , Plasmodium yoelii/immunology , Poly I-C/pharmacology , Rats , Rats, Wistar , Spleen/parasitologyABSTRACT
It is well known that Anopheles dirus is naturally refractory to rodent malaria parasite, Plasmodium yoelii, but the mechanism is still largely unknown. Here, we found that some P. yoelii taken into An. dirus could develop into oocysts, but oocysts were partially melanized at 7 days and completely melanized at 15 days post-infectious blood meal. Transmission electronic microscopy could find the melanized P. yoelii oocysts in An. dirus as early as 5 days post-infection, with a few haemocytes attaching to the melanized oocysts, indicating a typical humoral melanization reaction. Although the change of protein pattern at 24h post-infection suggested that other unknown mechanisms and/or factors might be involved in killing ookinetes, our data implied that oocysts melanization was one of the mechanisms of An. dirus to block P. yoelii development. In addition, activity of phenoloxidase, such as monophenol oxidase and o-diphenoloxidase, in haemolymph of An. dirus fed on infectious blood meal was much higher than that of mosquitoes fed on 5% glucose or normal mouse blood (p<0.05), implying the possible role of PO in oocysts melanization by An. dirus.
Subject(s)
Anopheles/parasitology , Insect Vectors/parasitology , Melanins/metabolism , Plasmodium yoelii/immunology , Animals , Anopheles/enzymology , Anopheles/metabolism , Electrophoresis, Polyacrylamide Gel , Female , Insect Vectors/enzymology , Insect Vectors/metabolism , Mice , Microscopy, Electron, Transmission , Monophenol Monooxygenase/metabolism , Oocysts/immunology , Oocysts/metabolism , Oocysts/ultrastructure , Plasmodium yoelii/metabolism , Plasmodium yoelii/ultrastructureABSTRACT
<p><b>OBJECTIVE</b>To investigate the characteristic of colonic transmission in functional constipation (FC) and the effect of traditional Chinese medicine (TCM) Sini Powder (SP) on it.</p><p><b>METHODS</b>The colonic transmission time (CTT) of 36 patients with FC (the FC group) and 22 healthy subjects (control group) was measured through colonic transmission test, and CTT of entire colon and that of various subsections was calculated with Hinton method and Arhan method respectively. After then, the FC group was treated with SP for 7 days, and CTT was detected again after treatment.</p><p><b>RESULTS</b>Before treatment, body mass index (BMI) was higher, CTT of entire colon, left half colonic section, and sigmoid-rectal section were longer in the FC group than those in the control group (P < 0.05), no statistical difference in CTT of right half colon was found between the two groups (P > 0.05). After FC patients being treated with SP, their CTT of whole colon, left half colonic section and sigmoid-rectal section were significantly shortened (P < 0.05).</p><p><b>CONCLUSION</b>FC patients were characterized by increased BMI and CTT prolonged and unevenly distributed in subsections, especially in the left half colon, sigmoid and rectum; SP could shorten the CTT in FC patients.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Body Mass Index , Colon , Colon, Sigmoid , Constipation , Drug Therapy , Drugs, Chinese Herbal , Therapeutic Uses , Gastrointestinal Transit , Phytotherapy , Time Factors , Treatment OutcomeABSTRACT
Objective To explore the relationship between hemolymph phenol oxidase and the melanization of Plasmodium yoelii oocysts in Anopheles dirus. Methods An Anopheles dirus-Plasmodium yoelii system was used Anopheles dirus were divided into 3 groups, that is, non-blood-fedding (N), normal-blood-fedding (B) and infected-blood-fedding (I). The activities of MPO and o-DPO in hemolymph from 3 groups were determined with native polyacrylamide gel electrophoresis (PAGE) and density scanning at 5, 7, 11 and 15 d after blood feeding. Results Both MPO and o-DPO activity were significantly higher in group I than group N and B (P<0.05). But with the melanization of Plasmodium yoelii oocysts, both MPO and o-DPO activity in group I were decreased in comparison with group N, especially on the 15 th day after infected-blood feeding. MPO and o-DPO activity in group B were significantly stronger than those of group N. Conclusion Blood feeding and infection of Plasmodium yoelii both can activate the cascade. The heamolymph phenol oxidase may play an important role in the melanization of Plasmodium yoelii oocysts in Anopheles dirus.
ABSTRACT
Objective To explore the relationship between hemolymph phenol oxidase and the melanization of Plasmodium yoelii oocysts in Anopheles dirus. Methods An Anopheles dirus-Plasmodium yoelii system was used Anopheles dirus were divided into 3 groups, that is, non-blood-fedding (N), normal-blood-fedding (B) and infected-blood-fedding (I). The activities of MPO and o-DPO in hemolymph from 3 groups were determined with native polyacrylamide gel electrophoresis (PAGE) and density scanning at 5, 7, 11 and 15 d after blood feeding. Results Both MPO and o-DPO activity were significantly higher in group I than group N and B (P<0.05). But with the melanization of Plasmodium yoelii oocysts, both MPO and o-DPO activity in group I were decreased in comparison with group N, especially on the 15 th day after infected-blood feeding. MPO and o-DPO activity in group B were significantly stronger than those of group N. Conclusion Blood feeding and infection of Plasmodium yoelii both can activate the cascade. The heamolymph phenol oxidase may play an important role in the melanization of Plasmodium yoelii oocysts in Anopheles dirus.
