A population genetic study of 22 autosomal loci of single nucleotide polymorphisms / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To evaluate polymorphisms and forensic efficiency of 22 non-binary single nucleotide polymorphism (SNP) loci.</p><p><b>METHODS</b>One hundred ethnic Han Chinese individuals were recruited from Dongguan, Guangdong. The 22 loci were genotyped with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).</p><p><b>RESULTS</b>Nine loci were found with a single allele, 4 loci were found to be biallelic, whilst 9 loci were found to have 3 alleles. For 13 polymorphic loci, the combined discrimination power and power of exclusion were 0.999 98 and 0.9330, respectively. For the 9 non-biallelic loci, the combined discrimination power and power of exclusion were 0.9998 and 0.8956, respectively. For motherless cases, the combined power of exclusion was 0.6405 for 13 polymorphic SNPs and 0.6405 for 9 non-binary SNPs.</p><p><b>CONCLUSION</b>Non-binary loci have a greater discrimination power and exclusion power per SNP.</p>

Genetic polymorphisms of fifteen short tandem repeat loci in Guilin Han population / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To investigate the genetic polymorphisms of 15 short tandem repeat (STRs) in Guilin Han population.</p><p><b>METHODS</b>DNA was extracted by Chelex method, and 15 STRs were analyzed using AmpFISTR Identifiler kit.</p><p><b>RESULTS</b>Four rare alleles, namely FGA * 10, D2S1338 * 10, D3S1358 * 16.2 and D3S1358 * 17.2, were observed. The combined match probability and exclusion probability for the 15 STRs were 2.89 x 10(-17) and 0.9999993, respectively.</p><p><b>CONCLUSION</b>These STRs have good discrimination power and exclusion probability in Guilin Han population.</p>

Distributions of haplotypes for three Y-STR loci in a Tibetan ethnic group of Chinese population by using Y-STR multiplexes / 法医学杂志

OBJECTIVE@#One multiplex genotyping system was developed in using silver staining with allelic ladders for three Y-chromosome STR markers (DYS434, DYS443, and DYS456), with a view towards the application of rapid and simple genotyping assay methods for DNA profiling. The distributions of haplotypes for three Y-STR loci(DYS434, DYS443, and DYS456) was investigated in a Tibetan ethnic group of Chinese population.@*METHODS@#Allele and haplotype frequencies at these Y-STRs loci(DYS434, DYS443, and DYS456) were analysed by PCR amplification using Y-STR multiplexes, followed by horizontal non-denaturing polyacrylamide gelelec-trophoresis in 101 unrelated males of Tibetan ethnic group in Lasa of China.@*RESULTS@#A total of 31 different haplotypes were found, 16 of them being unique. The haplotype diversity value (which is the same as the discrimination index) calculated from all three loci combined was 0.9481, which is informative.@*CONCLUSION@#The Y-STR multiplexes provide useful information for forensic analysis and paternity tests and can also be of great benefit for providing information not normally available from autosomal DNA systems.