ABSTRACT
Objective: To investigate the correlation between genetic polymorphisms of cystathionine β-synthase and serum homocysteine in patients with cerebral infarction. Methods: CBS T833C polymorphisms were detected by ARMS and serum homocysteine were measured with HPLC in 67 patients with cerebral infarction and 31 controls. Results: In patients group, CBS genetic homozygous and heterozygous mutations were found in 9 cases and 28 cases respectively. However, there were 3 and 5 cases respectively in the control group. There was significant difference in the frequencies of genotypes and alleles between the two groups (P < 0.01). The levels of serum homocysteine were markedly higher in the patients with CBS genetic heterozygous mutation than that of other patients (t = 4.612, P < 0.01). The mean serum homocysteine level was higher in patients with cerebral infarction than that in controls (23 ± 7 versus 13 ± 4 μmol/L, t = 8.826, P < 0.01). Conclusion: Hyperhomocysteinemia is an independent risk factor for cerebrovascular diseases. Point mutation of CBS gene T833C may play important part in the mechanism of hyperhomocysteinemia and cerbral infarction.
ABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa.</p><p><b>METHOD</b>The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm.</p><p><b>RESULT</b>The assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration.</p><p><b>CONCLUSION</b>The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.</p>
