Effects of preseason training on body composition, running performance, biochemical markers and workload variation in professional rugby union players.

Few studies have examined the impact of a preseason training intervention through systematic measures in Pro D2 rugby union (RU). Therefore, this study aimed to describe the effects of 12 weeks of preseason training (three blocks) on body composition, running performance, biochemical markers, and workload (WL) variation in professional RU players. Physiological (physical and biochemical) responses to preseason WL were analyzed by examining changes in anthropometric characteristics, Yo-Yo intermittent recovery level 1 (Yo-Yo IR1) test, blood samples (BS), Hooper index (1-7), the 10-Hz global positioning system (GPS), and session rating of perceived exertion (s-RPE) in nineteen elite male players. Changes throughout the preseason were analyzed using the one-way and mixed-model analysis of variance. Significant (p < 0.01) improvements occurred in anthropometry and Yo-Yo IR1 running performance in forwards and backs. Total distance (p < 0.01) and impact (p < 0.05) during the second block were meaningfully higher than the other two blocks, with backs showing higher values than forwards. As expected, WL decreased significantly (p < 0.01) during the last training block. The WL variations were correlated with changes in biochemical markers over the preseason period. The collected data can be used for i) profiling French Pro D2 rugby championships players, ii) establishing effective training strategies, and iii) setting preseason WL expectations.

Insights into the relationship between denitrification and organic carbon release of solid-phase denitrification systems: Mechanism and microbial characteristics.

Solid-phase denitrification is a promising alternative denitrification technology when facing a shortage of carbon sources. Nevertheless, it is still unclear whether there is a certain interaction between the denitrification process and the carbon release process in a solid-phase denitrification system. In this study, the concept of "Self-adaptation" was proposed for the relationship between denitrification and carbon release. At various influent nitrate loads, the PCL-supported denitrification system achieved an average nitrate removal rate of over 90.59 ± 7.01 % and a maximum denitrification rate of 0.67 ± 0.06 gN/(L·d). Microorganisms can spontaneously regulate the carbon release rate of PCL in response to changes in influent nitrate load, demonstrating "self-adaptation" of the PCL-supported solid-phase denitrification system. Regulation of carbon release rate via the "Self-adaptation" was achieved by changes in extracellular depolymerase activity. Acidovorax_sp. played a key role in "Self-adaptation", for its function of both denitrification and PCL degradation.