Dendron-Functionalized Surface: Efficient Strategy for Enhancing the Capture of Microvesicles.

Microvesicles (MVs) are used by various types of cells in the human body for intercellular communication, making them biomarkers of great potential for the early and non-evasive diagnosis of a spectrum of diseases. An integrated analysis including morphological, quantitative, and compositional studies is most desirable for the clinical application of MV detection; however, such integration is limited by the currently available analysis techniques. In this context, exploiting the phosphatidylserine (PS) exposure of MVs, we synthesized a series of dendritic molecules with PS-binding sites at the periphery. PS-dendron binding was studied at the molecular level using NMR approaches, whereas PS-containing membrane-dendron interaction was investigated in an aqueous environment using plasmon waveguide resonance spectroscopy. As a proof of concept, polyethylene terephthalate surface was functionalized with the synthetic dendrons, forming devices that can capture MVs to facilitate their subsequent analyses.

Ultrabright organic fluorescent microparticles for in vivo tracing applications.

We report the in vivo distribution, toxicity and metabolism of micro-sized fluorescent organic particles and their applications in cerebral blood flow tracing. The fluorescent microparticles exhibit bright fluorescence, good photo-stability and low toxicity; therefore, they are ideal for long-term non-invasive in vivo tracing. In contrast to conventional fluorescent labeling agents, which stain the entire blood vessel, the tracer microparticles can be easily tracked individually and provide vital information about blood flow behavior. Furthermore, we observed stimulated emission from these microparticles in living animals. These microparticles can provide unprecedented contrast for simultaneous observation of the distribution of blood vessels and the dynamics of microcirculation. Pathological examination revealed that the injected microparticles eventually collected in the spleen and liver. We found no observable toxicity of the microparticles to cells or mouse organs. We demonstrate that these fluorescent microparticles are suitable for applications in the field of non-intrusive blood flow tracing and could play a complementary role to traditional imaging agents.

Rational design of small indolic squaraine dyes with large two-photon absorption cross section.

Small organic dyes with large two-photon absorption (TPA) cross sections (δ) are more desirable in many applications compared with large molecules. Herein, we proposed a facile theoretical method for the fast screening of small organic molecules as potential TPA dyes. This method is based on a theoretical analysis to the natural transition orbitals (NTOs) directly associated with the TPA transition. Experimental results on the small indolic squaraine dyes (ISD) confirmed that their TPA cross sections is strongly correlated to the delocalization degree of the NTOs of the S2 excited states. Aided by this simple and intuitive method, we have successfully designed and synthesized a small indolic squaraine dye (ISD) with a remarkable δ value above 8000 GM at 780 nm. The ISD dye also exhibits a high singlet oxygen generation quantum yield about 0.90. The rationally designed TPA dye was successfully applied in both two-photon excited fluorescence cell imaging and in vivo cerebrovascular blood fluid tracing.

New molecular probe for the selective detection of zinc ion.

A new multisignaling molecular probe DFDB was designed for the selective detection of Zn(2+). DFDB can be synthesized by a simple one-step reaction in high yield. Theoretical calculation suggests a novel sandwich structure of the DFDB·Zn(2+) complex.