Purification of inorganic nitrogen from the mariculture tail water by anaerobic/anoxic/oxic (A2O) process.

This study aims to address the suboptimal performance of conventional denitrifying strains in treating mariculture tail water (MTW) containing inorganic nitrogen (IN). The concentration of inorganic nitrogen in the mariculture tail water is about 5-20 mg·L-1. A biofilm treatment process was developed and evaluated using an anoxic-anoxic-aerobic biofilter composite system inoculated with the denitrifying strain Meyerozyma guilliermondii Y8. The removal effect of total nitrogen (TN), IN, and Chemical Oxygen Demand (CODMn) from MTW was investigated. The results indicate that the A2O composite biological filter has excellent pollutant removal efficiency within 25 days of operation, after the acclimation of the denitrifying microorganisms. The initial concentrations of TN, IN, and CODMn ranged between 10.24 and 12.89 mg·L-1, 7.84-10.49 mg·L-1, and 9.44-11.52 mg·L-1, respectively, and the removal rates of these indexes reached 38-68 %, 45-70 %, and 55-70 %, respectively. The experiments with different hydraulic retention times (HRT = 6 h, 8 h, 10 h) demonstrated that longer HRT was more conducive to the removal of inorganic nitrogen. Moreover, scanning electron microscopy observations revealed that the target strain successfully grew and attached to the filler in large quantities. The findings of this study provide practical guidance for the development of efficient biofilm processes for the treatment of MTW.

Aerobic denitrifying bacterial-fungal consortium mediating nitrate removal: Dynamics, network patterns and interactions.

In recent years, nitrogen removal by mixed microbial cultures has received increasing attention owing to cooperative metabolism. A natural bacterial-fungal consortium was isolated from mariculture, which exhibited an excellent aerobic denitrification capacity. Under aerobic conditions, nitrate removal and denitrification efficiencies were up to 100% and 44.27%, respectively. High-throughput sequencing and network analysis suggested that aerobic denitrification was potentially driven by the co-occurrence of the following bacterial and fungal genera: Vibrio, Fusarium, Gibberella, Meyerozyma, Exophiala and Pseudoalteromonas, with the dominance of Vibrio and Fusarium in bacterial and fungal communities, respectively. In addition, the isolated consortium had a high steady aerobic denitrification performance in our sub-culturing experiments. Our results provide new insights on the dynamics, network patterns and interactions of aerobic denitrifying microbial consortia with a high potential for new biotechnology applications.

A novel adsorbent based on aptamer prepared via "thiol-ene" click for specific recognition of phthalic acid esters.

In this paper, a novel adsorbent based on aptamer was prepared via "thiol-ene" click chemistry reaction and used for selective adsorbing the trace phthalic acid esters (PAEs) from drinking water and juice samples, which depended on the group selectivity of aptamers to the ester and the benzoyl groups of PAEs. The morphological structures of the obtained adsorbents were characterized by Fourier Transform infrared spectroscopy (FT-IR), fluorescence spectra, Energy Dispersive Spectrometer (EDS), Brunauer-Emmett-Teller (BET). The selectivity of the prepared adsorbent was evaluated and the results showed that the recovery of the adsorbent with aptamer for PAEs was 66.10-108.90%, while the recovery of adsorbent without aptamer was only 32.41-37.59%. The limit of detection (LOD) (S/N = 3) and limit of quantitation (LOQ) (S/N = 10) of PAEs coupled with HPLC-UV were obtained in the range of 0.11-0.88 µg L-1 and 0.22-1.33 µg L-1, respectively. This work gave a facile and efficient approach to for specific enrichment and highly sensitivity detection of PAEs.

Microplastic pollution and ecological risk assessment in an estuarine environment: The Dongshan Bay of China.

Microplastic (MP) pollution has spurred a wide range of concerns due to its ubiquity and potential hazards to humans and ecosystems, yet studies on MP abundance, distribution, and ecological impacts on the small-scale local estuarine systems are insufficient. We conducted the first study of MP pollution in surface water of Dongshan Bay in southern China. A total of six water samples were collected using a Manta trawl (length = 3 m, width = 1 m, height = 0.6 m, and mesh size = âˆ¼330 µm). The abundance, type, shape, color, and size, were measured using light microscopy and micro-Raman spectroscopy. Our results showed that MPs spanned from 0.23 to 4.01 particles m-3 with an average of 1.66 particles m-3. 75% of the MPs were PP, PE, and PS that may be explained by the widespread application of PE, PP, and PS foam in local fishing and aquaculture within the bay. Foam, white, and 1.0-2.5 mm were dominant shape, color, and size of MPs, respectively. Both indices of MPs-induced risk (Hestuary = 13.7) and pollution load (PLIestuary = 14.2) yielded a Hazard Level II for MPs pollution in the Dongshan Bay. The potential ecological risk from combined MPs polymers (RIestuary = 21.5) ended up at a minor risk. Our findings established the first set of baseline data on MPs pollution in Dongshan Bay and provided preliminary quantitative measures on the scale of ecological risk, which would improve the understanding of MP fate, transport, and ecological impacts in the estuarine environment.