ABSTRACT
The purpose of this study was to investigate the antimicrobial activity and mechanisms of Nα-lauroyl arginate ethyl ester (LAE) against Penicillium digitatum and Pectobacterium carotovorum subsp. carotovorum. The minim inhibitory concentrations of LAE against P. digitatum and P. carotovorum were found to be 400 and 25 µg/ml, respectively. Loss of intracellular protein and nucleic acid increased significantly, and membrane permeability reached 76.28, 54.29 and 85.20%, respectively, when 400 µg/ml of LAE was applied to the hyphae and spores of P. digitatum and to P. carotovorum. Flow cytometry showed that LAE reduced the membrane potential, and the depolarization ratios of P. digitatum and P. carotovorum were 98.19 and 97.25% (P < 0.05), respectively. Transmission electron microscopy photos revealed that LAE caused a rough surface, irregular cellular organelles, protoplast shrinkage, intracytoplasmic coagulation and empty cavities in all three cell types. These results showed that LAE had notable ability to damage the structure of fungal and bacterial cells, making it a possible alternative chemical for use in the preservation of fruits and vegetables.
ABSTRACT
The objective of this study was to evaluate in vitro antioxidant capacities of polysaccharides extracted from Moso Bamboo-Leaf (BLP). The molecular weight of the polysaccharides were obtained by SEC-LLS and their molecular masses were 13.4×10(4)Da, 2.8×10(4)Da and 1.9×10(4)Da, respectively. Available data obtained with in vitro models suggested WB1 (precipitated by 30% ethanol) showed significant inhibitory effects on superoxide radical, hydroxyl radical, DPPH radical and ferrous metal-chelating capacities. These results clearly establish the possibility that polysaccharides extracted from Moso Bamboo-Leaf could be effectively employed as ingredient in health or functional food, to alleviate oxidative stress.
