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1.
ACS Omega ; 8(46): 43903-43919, 2023 Nov 21.
Article in English | MEDLINE | ID: mdl-38027315

ABSTRACT

Characterizing the pore and fluid distribution is critical for evaluating the reservoir potential of new areas. Nuclear magnetic resonance (NMR) is considered as an experimental method capable of full-scale characterization of pore characteristics. However, the T2 spectrum of a saturated sample is affected by a combination of sample and experimental parameters, and it is important to confirm whether the T2 spectrum fully reflects the sample pore information. Eight tight sandstone samples from the Julu area were selected for thin section identification, mercury intrusion porosimetry (MIP), NMR, NMR cryoporometry (NMRC), and centrifugation experiments to critically analyze the applicability of the NMR results. Two methods, the similarity method and Kozeny's equation method, were used to calculate surface relaxivity, a critical parameter for converting NMR T2 signals into pore information. The discussion focuses on the applicability of the calculated surface relaxivity and the phenomenon of T2 signal changes in a short relaxation range after centrifugation. The main results are as follows: The surface relaxivity values calculated using the different methods differed significantly. The surface relaxivity calculated using the same method reflected the relative magnitude of the true surface relaxivity of the samples. For the samples with large surface relaxivity, there may be partial misses of the short relaxation signal, the NMR porosity was smaller than the gas-measured porosity, there was a variation in the T2 spectrum in the short relaxation range after centrifugation, and the calculated surface relaxivity was small. The surface relaxivity calculated using Kozeny's equation was nearly accurate, but perhaps smaller than the true value. The T2 spectra mainly reflected macropore information. This study suggests that PSDs converted from T2 spectra of saturated samples should be judged with relative caution rather than solely based on the peak or range correspondence between the two curves, and the minimum centrifugal radius can be used as a constraint.

2.
Forensic Sci Res ; 8(3): 230-240, 2023 Sep.
Article in English | MEDLINE | ID: mdl-38221964

ABSTRACT

MicroRNAs (miRNAs) are a class of small non-coding RNAs that exert their biological functions as negative regulators of gene expression. They are involved in the skin wound healing process with a dynamic expression pattern and can therefore potentially serve as biomarkers for skin wound age estimation. However, no reports have described any miRNAs as suitable reference genes (RGs) for miRNA quantification in wounded skin or samples with post-mortem changes. Here, we aimed to identify specific miRNAs as RGs for miRNA quantification to support further studies of skin wound age estimation. Overall, nine miRNAs stably expressed in mouse skin at certain posttraumatic intervals (PTIs) were preselected by next-generation sequencing as candidate RGs. These nine miRNAs and the commonly used reference genes (comRGs: U6, GAPDH, ACTB, 18S, 5S, LC-Ogdh) were quantitatively examined using quantitative real-time reverse-transcription polymerase chain reaction at different PTIs during skin wound healing in mice. The stabilities of these genes were evaluated using four independent algorithms: GeNorm, NormFinder, BestKeeper, and comparative Delta Ct. Stability was further evaluated in mice with different post-mortem intervals (PMIs). Overall, mmu-miR-26a-5p, mmu-miR-30d-5p, and mmu-miR-152-3p were identified as the most stable genes at both different PTIs and PMIs. These three miRNA RGs were additionally validated and compared with the comRGs in human samples. After assessing using one, two, or three miRNAs in combination for stability at different PTIs, PMIs, or in human samples, the set of miR-26a/30d/152 was approved as the best normalizer. In conclusion, our data suggest that the combination of miR-26a/30d/152 is recommended as the normalization strategy for miRNA qRT-PCR quantification in skin wound age estimation. Key points: The small size of miRNAs makes them less susceptible to post-mortem autolysis or putrefaction, leading to their potential use in wound age estimation.Studying miRNAs as biological indicators of skin wound age estimation requires the selection and validation of stable reference genes because commonly used reference genes, such as U6, ACTB, GAPDH, 5S, 18S, and LC-Ogdh, are not stable.miR-26a/30d/152 are stable and reliable as reference genes and their use in combination is a recommended normalization strategy for miRNA quantitative analysis in wounded skin.

3.
Global Spine J ; : 21925682221110180, 2022 Jun 18.
Article in English | MEDLINE | ID: mdl-35719094

ABSTRACT

STUDY DESIGN: Retrospective cohort study. OBJECTIVE: To investigate the effect of HRVA on the intrapedicular accuracy of C2PS placement through the freehand method in patients with BI and analyse the possible risk factors for C2PS malpositioning. METHOD: A total of 91 consecutive patients with BI who received 174 unilateral C2PS placements through the freehand method were retrospectively included. The unilateral pedicles were assigned to the HRVA and non-HRVA groups. The primary outcome was the intrapedicular accuracy of C2PS placement in accordance with the Gertzbein-Robbins scale. Moreover, the risk factors that possibly affected intrapedicular accuracy were assessed. RESULTS: The rate of intrapedicular accuracy in C2PS placement in patients with BI was 23.6%. Results showed that the non-HRVA group had remarkably higher rates of optimal and clinically acceptable C2PS placement than the HRVA group. Nevertheless, the HRVA group exhibited similar results for grade B classification as the non-HRVA group. Moreover, in the HRVA and non-HRVA groups, the most common direction of screw deviations was the lateral direction. Furthermore, the multivariate analyses showed that the obliquity of the lateral atlantoaxial joint in the sagittal plane ≥15°, and that in the coronal plane ≥ 20°, isthmus height < 4.3 mm, and distance from the skin to the spinous process ≥ 2.8 cm independently contributed to a high rate of screw malpositioning in BI patients. CONCLUSION: The presence of HRVA in BI patients contributed to the high rate of malpositioning in C2PS placement via the freehand method. However, the rates of intrapedicular accuracy in patients with BI with and without HRVA were considerably low.

4.
Spine J ; 22(8): 1281-1291, 2022 08.
Article in English | MEDLINE | ID: mdl-35508287

ABSTRACT

BACKGROUND: C2 pedicle screw placement in patients with basilar invagination (BI) is fraught with risks because of a high incidence of anatomical variations and high-riding vertebral artery (HRVA). However, no study can be found in the literature that attempted to identify the ideal entry point and trajectory through the C2 pedicle in BI patients with HRVA. PURPOSE: To investigate the parameters of ideal entry point and trajectory for C2 pedicle screw placement in BI patients with HRVA and compare them with those in BI patients without HRVA and patients without BI as control. These parameters would serve as a guide to pedicle screw placement. STUDY DESIGN: A retrospective comparative study. PATIENT SAMPLE: A total of 396 patients (198 consecutive BI patients and 198 matched patients without BI as control) and 792 unilateral pedicles from April 2017 to October 2021 at two medical centers were included. OUTCOME MEASURES: The insertion parameters of mediolateral angle, surface distance, cephalad angle, and vertical distance from the superior border of the lamina were the primary outcome measures for the reference of C2 pedicle screw placement. Furthermore, factors that affect the primary insertion parameters were assessed via multiple linear regression analyses. METHOD: According to the diagnosis of BI and HRVA, the unilateral pedicles were assigned into HRVA of BI, non-HRVA of BI, HRVA of control, and non-HRVA of control groups. Subgroup analyses based on Goel types A and B were also performed. Moreover, vertebral artery (VA) anomalies that might result in potentially serious complications were identified and systematically compared. RESULTS: The measurements of insertion parameters in BI patients with HRVA indicated a mean mediolateral angle of 27.42°, a mean cephalad angle of 43.02°, a mean surface distance of 9.74 mm, and a mean vertical distance from the superior border of the lamina of 3.85 mm. Compared with that in BI patients without HRVA, the measurements suggested that the entry point in BI patients with HRVA should be shifted upward by 0.38 mm and the trajectory should be angled cephalad by 6.05° and medially by 4.78°. In the control group, changes in the insertion parameters between HRVA and non-HRVA showed a similar trend to the BI group. Multiple linear regression showed that mediolateral angle was significantly associated with the male gender (B=-0.930, p=.017) and the diagnoses of HRVA (B=6.964, p<.001), Goel type A (B=-1.656, p=.003), and Goel type B (B=0.981, p=.030). Moreover, cephalad angle was significantly associated with the length of lateral mass (B=-0.319, p=.001) and the diagnoses of HRVA (B=3.254, p<.001) and Goel type A (B=6.924, p<.001). The VA anomalies were significantly higher in the BI group than in the control group. CONCLUSIONS: The insertion parameters of the ideal entry point and trajectory for C2 screw placement in BI patients with HRVA were remarkably different from those of non-HRVA of BI, HRVA of control, and non-HRVA of control cohorts. Preoperative 3D computed tomography (CT) and CT angiography are highly recommended in such patients to improve intraoperative safety and reduce postoperative complications.


Subject(s)
Pedicle Screws , Spinal Fusion , Cervical Vertebrae/diagnostic imaging , Cervical Vertebrae/surgery , Humans , Male , Retrospective Studies , Spinal Fusion/methods , Tomography, X-Ray Computed , Vertebral Artery/diagnostic imaging , Vertebral Artery/surgery
5.
Neurospine ; 19(4): 899-911, 2022 Dec.
Article in English | MEDLINE | ID: mdl-36597627

ABSTRACT

OBJECTIVE: Patients with basilar invagination (BI) had high incidences of vertebral variations and high-riding vertebral artery (HRVA) that might restrict the use of pedicle or pars screw and increase the use of translaminar screw on axis. Here, we conducted a radiographic study to investigate the feasibility of translaminar screws and the bone quality of C2 laminae in patients with BI, which were compared with those without BI as control to provide guidelines for safe placement. METHODS: In this study, a total of 410 patients (205 consecutive patients with BI and 205 matched patients without BI) and 820 unilateral laminae of the axis were included at a 1:1 ratio. Comparisons with regard to insertion parameters (laminar length, thickness, angle, and height) for C2 translaminar screw placement and Hounsfield unit (HU) values for the assessment of the appropriate bone mineral density of C2 laminae between BI and control groups were performed. Besides, the subgroup analyses based on the Goel A and B classification of BI, HRVA, atlas occipitalization, and C2/3 assimilation were also carried out. Furthermore, the factors that might affect the insertion parameters and HU values were explored through multiple linear regression analyses. RESULTS: The BI group showed a significantly smaller laminar length, thickness, height, and HU value than the control group, whereas no significant difference was observed regarding the laminar angle. By contrast, the control group showed significantly higher rates of acceptability for unilateral and bilateral translaminar screw fixations than the BI group. Subgroup analyses showed that the classification of Goel A and B, HRVA, atlas occipitalization, and C2/3 assimilation affected the insertion parameters except the HU values. Multiple linear regression indicated that the laminar length was significantly associated with the male gender (B = 0.190, p < 0.001), diagnoses of HRVA (B = -0.109, p < 0.001), Goel A (B = -0.167, p < 0.001), and C2/3 assimilation (B = -0.079, p = 0.029); the laminar thickness was significantly associated with the male gender (B = 0.353, p < 0.001), diagnoses of HRVA (B = -0.430, p < 0.001), Goel B (B = -0.249, p = 0.026), and distance from the top of odontoid to the Chamberlain line (B = -0.025, p = 0.003); laminar HU values were significantly associated with age (B = -2.517, p < 0.001), Goel A (B = -44.205, p < 0.001), Goel B (B = -25.704, p = 0.014), and laminar thickness (B = -11.706, p = 0.001). CONCLUSION: Patients with BI had narrower and smaller laminae with lower HU values and lower unilateral and bilateral acceptability for translaminar screws than patients without BI. Preoperative 3-dimensional computed tomography (CT) and CT angiography were needed for BI patients.

6.
BMC Pregnancy Childbirth ; 21(1): 445, 2021 Jun 25.
Article in English | MEDLINE | ID: mdl-34172031

ABSTRACT

OBJECTIVE: To explore the risk factors for intrapartum fever and to develop a nomogram to predict the incidence of intrapartum fever. METHODS: The general demographic characteristics and perinatal factors of 696 parturients who underwent vaginal birth at the Affiliated Hospital of Xuzhou Medical University from May 2019 to April 2020 were retrospectively analysed. Data was collected from May 2019 to October 2019 on 487 pregnant women who formed a training cohort. A multivariate logistic regression model was used to identify the independent risk factors associated with intrapartum fever during vaginal birth, and a nomogram was developed to predict the occurrence. To verify the nomogram, data was collected from January 2020 to April in 2020 from 209 pregnant women who formed a validation cohort. RESULTS: The incidence of intrapartum fever in the training cohort was found in 72 of the 487 parturients (14.8%), and the incidence of intrapartum fever in the validation cohort was 31 of the 209 parturients (14.8%). Multivariate logistic regression analysis showed that the following factors were significantly related to intrapartum fever: primiparas (odds ratio [OR] 2.43; 95% confidence interval [CI] 1.15-5.15), epidural labour analgesia (OR 2.89; 95% CI 1.23-6.82), premature rupture of membranes (OR 2.37; 95% CI 1.13-4.95), second stage of labour ≥ 120 min (OR 4.36; 95% CI 1.42-13.41), amniotic fluid pollution degree III (OR 10.39; 95% CI 3.30-32.73), and foetal weight ≥ 4000 g (OR 7.49; 95% CI 2.12-26.54). Based on clinical experience and previous studies, the duration of epidural labour analgesia also appeared to be a meaningful factor for intrapartum fever; therefore, these seven variables were used to develop a nomogram to predict intrapartum fever in parturients. The nomogram achieved a good area under the ROC curve of 0.86 and 0.81 in the training and in the validation cohorts, respectively. Additionally, the nomogram had a well-fitted calibration curve, which also showed excellent diagnostic performance. CONCLUSION: We constructed a model to predict the occurrence of fever during childbirth and developed an accessible nomogram to help doctors assess the risk of fever during childbirth. Such assessment may be helpful in implementing reasonable treatment measures. TRIAL REGISTRATION: Clinical Trial Registration: ( www.chictr.org.cn ChiCTR2000035593 ).


Subject(s)
Fever/diagnosis , Nomograms , Obstetric Labor Complications/diagnosis , Prenatal Diagnosis/methods , Risk Assessment/methods , Adult , Analgesia, Epidural/adverse effects , Case-Control Studies , Delivery, Obstetric/adverse effects , Delivery, Obstetric/methods , Female , Fever/epidemiology , Fever/etiology , Humans , Incidence , Logistic Models , Obstetric Labor Complications/epidemiology , Obstetric Labor Complications/etiology , Odds Ratio , Parity , Parturition , Predictive Value of Tests , Pregnancy , Prenatal Diagnosis/standards , Retrospective Studies , Risk Factors , Young Adult
7.
Eur J Anaesthesiol ; 38(3): 285-293, 2021 03 01.
Article in English | MEDLINE | ID: mdl-33186312

ABSTRACT

BACKGROUND: Sore throat is a common complication after Laryngeal Mask Airway Supreme (SLMA) insertion. OBJECTIVE: The aim of this study was to determine whether a new SLMA insertion technique (not removing the pilot tube blocker before insertion) lowers the incidence of sore throat in the postanaesthesia care unit (PACU). DESIGN: A prospective, single-centre, parallel randomised controlled trial. SETTING: Operating room and PACU at a hospital in China from June to September 2019. PATIENTS: Four hundred and eight patients aged 18 to 65 years with American Society of Anaesthesiologists physical status class I or II who were scheduled for elective surgery requiring anaesthesia and SLMA insertion. INTERVENTIONS: Leaving the blocker at the end of the pilot tube in situ (this blocker keeps the valve open and the balloon remains partially inflated but will deflate with pressure) or removing the blocker and actively deflating the cuff before SLMA insertion. MAIN OUTCOME MEASURES: The primary outcome was the incidence of postoperative sore throat in the PACU. The secondary outcomes included sore throat severity (Prince Henry Hospital Pain Score), first-attempt success rate, ease of insertion, time to successful SLMA insertion, oropharyngeal leak pressure, grade of view on fibreoptic bronchoscopy (indicating the accuracy of SLMA positioning) and adverse events. RESULTS: The incidence of sore throat was 33/204 (16.2%) in the nonremoval group, and 65/204 (31.9%) in the removal group (P < 0.001). The first-attempt success rate was 174/204 (85.3%) in the nonremoval group and 150/204 (73.76%) in the removal group (P = 0.003; relative risk 1.160, 95% CI 1.049 to 1.282). The Kaplan--Meier curves showed that the insertion time in the nonremoval group was shorter (log-rank P = 0.01). CONCLUSION: The new insertion technique, leaving the blocker attached to the end of the pilot balloon, resulted in a reduced incidence and severity of postoperative sore throat in the PACU, and an improved first-attempt success rate and the accuracy of SLMA positioning. TRIAL REGISTRATION: Chinese Clinical Trial Registry identifier: ChiCTR1900023022.


Subject(s)
Laryngeal Masks , Pharyngitis , Adolescent , Adult , Aged , China/epidemiology , Humans , Incidence , Laryngeal Masks/adverse effects , Middle Aged , Pharyngitis/diagnosis , Pharyngitis/epidemiology , Pharyngitis/etiology , Prospective Studies , Young Adult
8.
Drug Des Devel Ther ; 14: 3509-3518, 2020.
Article in English | MEDLINE | ID: mdl-32943843

ABSTRACT

OBJECTIVE: Propofol for procedural sedation and analgesia (PSA) for colonoscopy can result in a high prevalence of severe respiratory depression. Studies have shown that intravenous (IV) infusion of lidocaine can reduce propofol requirements significantly and increase the ventilatory response to carbon dioxide in humans. We tested the hypothesis that IV lidocaine could improve propofol-induced respiratory depression in obese patients during colonoscopy. METHODS: Ninety obese patients scheduled for painless colonoscopy were randomized to receive lidocaine (1.5 mg/kg, then 2 mg/kg/h, IV) or the same volume of 0.9% saline. Intraoperative sedation was provided by propofol. The primary outcome was the number of oxygen-desaturation episodes. Secondary outcomes were: the number of apnea episodes; total propofol consumption; time to the first hypoxia episode; time to consciousness loss; intraoperative hemodynamic parameters; awakening time; adverse events; duration of post-anesthesia care unit (PACU) stay; satisfaction of endoscopists and patients. RESULTS: Demographic characteristics between the two groups were comparable. The number of oxygen-desaturation episodes in group L (1.49±1.12) decreased by 0.622 (P=0.018) compared with that in group N (2.11±1.32), and the number of apnea episodes in group L decreased by 0.533 (P<0.001). Kaplan-Meier curves showed that the median time to the first hypoxia episode was longer in group L (86.78 s) than that in group N (63.83 s) (Log rank P=0.0008). The total propofol consumption, awakening time, and duration of PACU stay were reduced in group L. There was no significant difference in the prevalence of adverse events (P>0.05 for all). Satisfaction scores for endoscopists and patients in group L were higher than that in group N (P<0.001). CONCLUSION: Intravenous infusion of lidocaine could significantly reduce the number of oxygen-desaturation and apnea episodes in obese patients during painless colonoscopy. This method is worthy of clinical promotion. CLINICAL TRIALS REGISTRATION: ChiCTR2000028937.


Subject(s)
Anesthetics, Intravenous/pharmacology , Lidocaine/pharmacology , Obesity/drug therapy , Respiratory Insufficiency/drug therapy , Voltage-Gated Sodium Channel Blockers/pharmacology , Adolescent , Adult , Aged , Anesthetics, Intravenous/administration & dosage , Colonoscopy , Double-Blind Method , Female , Humans , Lidocaine/administration & dosage , Male , Middle Aged , Obesity/surgery , Prospective Studies , Respiratory Insufficiency/surgery , Voltage-Gated Sodium Channel Blockers/administration & dosage , Young Adult
9.
Sci Rep ; 10(1): 12867, 2020 07 30.
Article in English | MEDLINE | ID: mdl-32733059

ABSTRACT

Lacquer sap is a water-in-oil natural emulsion with high viscosity. In nature, it exudes from the phloem of lacquer tree to repair its wounds in the presence of O2. So far, it is unclear how rapid and smooth polymerization of urushiol is achieved in such a viscous sap. Here, we find that there is a diffuse interface layer with 2.43 nm of thickness between two phases. The interface layer consists of urushiol, urushiol-laccase complex, urushiol-stellacyanin complex and water-insoluble glycoprotein. Polymerization of urushiol is realized by multicomponent synergistic effect. Radicals are first formed by laccase-catalyzed oxidation of urushiol at the interface layer, then are transferred to the urushiol oil phase via wate-insoluble glycoprotein and initiate the polymerization of urushiol there. Stellacyanin inhibits the formation of certain radicals and controls the concentration of phenoxy radicals at the interface layer. Through the inhibition of radicals by stellacyanin and the electron transfer mediated by water-insoluble glycoprotein, the polymerization of urushiol at the interface layer is inhibited. This ensures that O2 can continuously penetrate into the aqueous phase to oxidize the reduced laccase so that the urushiol polymerization can continue smoothly. This polymerization mechanism provides an idea for developing new chemical reaction systems.

10.
J Opt Soc Am A Opt Image Sci Vis ; 36(6): 1033-1038, 2019 Jun 01.
Article in English | MEDLINE | ID: mdl-31158134

ABSTRACT

The degree of paraxiality (DOP) of an electromagnetic multi-Gaussian Schell-model (EM-MGSM) beam was discussed, and the dependence of DOP on the characteristics of beam sources was investigated. It is shown that the parameters of beam sources, including the rms widths of the auto-correlation functions, the degree of polarization, and the boundary characteristics, play an important role in the DOP of the beam. To explain these phenomena, the far-zone divergence angle of the EM-MGSM beam was further discussed.

11.
J Opt Soc Am A Opt Image Sci Vis ; 36(3): 443-449, 2019 Mar 01.
Article in English | MEDLINE | ID: mdl-30874181

ABSTRACT

The far-zone behavior of polychromatic light waves on scattering from an isotropic semi-soft-boundary medium with spectral dependence is considered, and both spectral shifts and spectral switches of the scattered field have been discussed. It is shown that the spectral behavior of the far-zone scattered field is influenced by the structural function width of the dielectric susceptibility of the scattering medium. In particular, the structural function width of the dielectric susceptibility of the scattering medium plays an important role in the direction at which the spectral switch occurs. These results may have potential applications in areas such as the inverse scattering problem.

12.
Eur J Neurosci ; 50(2): 1981-1993, 2019 07.
Article in English | MEDLINE | ID: mdl-30828870

ABSTRACT

Nrf2 plays a pivotal role in antioxidant response and anti-inflammation after traumatic brain injury (TBI), and its deletion aggravates TBI-induced brain damage. Previous studies have demonstrated that Nrf2 is activated post TBI, but dynamic changes in expression and cell type-specific characteristics remain unclear. In this study, the Feeney weight-drop contusion model was conducted to mimic TBI, and the ipsilateral cerebral cortex was collected at 1, 3, 7 and 14 days post TBI (dpi). Nrf2 protein levels were observed by western blot. Cell type-specific localization of Nrf2 after TBI was detected at different time intervals by double immunofluorescence staining. NeuN, GFAP, IBA1 and NG2 were used as cell type-specific markers to neurons, astrocytes, microglia and NG2 glia, respectively. After TBI, Nrf2 protein levels peaked at 1 dpi. Robust transient Nrf2 accumulation was co-localized with neurons, which was predominant at 1 dpi. Continuous weak Nrf2 expression was detected in activated astrocytes, and the number of double positive cells peaked at 7 dpi. Inducible widespread immunostaining of Nrf2 was observed in the nucleus of the microglia, and the number of Nrf2+ microglia peaked at 7 dpi. In addition, we also explored colocalization of Nrf2 in NG2 glia, in which the percentage of Nrf2+ in NG2 glia reached a climax at 3 dpi. This study reveals that the accumulation of endogenous Nrf2 might mediate different pathophysical roles in neurons and glias after TBI, the cell-type specific and time-dependent expression provide insights to explain the roles of Nrf2 in different neural cells.


Subject(s)
Brain Contusion/metabolism , Cerebral Cortex/metabolism , NF-E2-Related Factor 2/metabolism , Neuroglia/metabolism , Neurons/metabolism , Animals , Disease Models, Animal , Male , Mice , Mice, Inbred C57BL
13.
J Opt Soc Am A Opt Image Sci Vis ; 35(6): 1034-1038, 2018 Jun 01.
Article in English | MEDLINE | ID: mdl-29877349

ABSTRACT

The degree of paraxiality of an anisotropic generalized multi-Gaussian Schell-model (GMGSM) beam is discussed and shown to be affected by the anisotropy and boundary characteristics of the source. Numerical results are presented to show the influence of these factors.

14.
Toxicol Appl Pharmacol ; 346: 28-36, 2018 05 01.
Article in English | MEDLINE | ID: mdl-29571711

ABSTRACT

Traumatic brain injury (TBI), which leads to high mortality and morbidity, is a prominent public health problem worldwide with no effective treatment. Curcumin has been shown to be beneficial for neuroprotection in vivo and in vitro, but the underlying mechanism remains unclear. This study determined whether the neuroprotective role of curcumin in mouse TBI is dependent on the NF-E2-related factor (Nrf2) pathway. The Feeney weight-drop contusion model was used to mimic TBI. Curcumin was administered intraperitoneally 15 min after TBI induction, and brains were collected at 24 h after TBI. The levels of Nrf2 and its downstream genes (Hmox-1, Nqo1, Gclm, and Gclc) were detected by Western blot and qRT-PCR at 24 h after TBI. In addition, edema, oxidative damage, cell apoptosis and inflammatory reactions were evaluated in wild type (WT) and Nrf2-knockout (Nrf2-KO) mice to explore the role of Nrf2 signaling after curcumin treatment. In wild type mice, curcumin treatment resulted in reduced ipsilateral cortex injury, neutrophil infiltration, and microglia activation, improving neuron survival against TBI-induced apoptosis and degeneration. These effects were accompanied by increased expression and nuclear translocation of Nrf2, and enhanced expression of antioxidant enzymes. However, Nrf2 deletion attenuated the neuroprotective effects of curcumin in Nrf2-KO mice after TBI. These findings demonstrated that curcumin effects on TBI are associated with the activation the Nrf2 pathway, providing novel insights into the neuroprotective role of Nrf2 and the potential therapeutic use of curcumin for TBI.


Subject(s)
Brain Injuries, Traumatic/drug therapy , Curcumin/pharmacology , NF-E2-Related Factor 2/metabolism , Neuroprotective Agents/pharmacology , Signal Transduction/drug effects , Animals , Antioxidants/metabolism , Apoptosis/drug effects , Brain/drug effects , Brain/metabolism , Brain Injuries, Traumatic/metabolism , Disease Models, Animal , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Oxidative Stress/drug effects
15.
Inflammation ; 41(2): 474-484, 2018 Mar.
Article in English | MEDLINE | ID: mdl-29196961

ABSTRACT

The α7 nicotinic acetylcholine receptor (α7-nAChR) is associated with inflammation, re-epithelialization, and angiogenesis in wound healing process. A recent study demonstrated that PNU-282987, a selective agonist of α7-nAChR, accelerates the repair of diabetic excisional wounds. Whether α7-nAChR activation promotes non-diabetic wounds healing is unknown. The aim of this study was to evaluate the effects of α7-nAChR activation on non-diabetic wound healing. The effects were evaluated in two wound models. In the first model, the wound was covered with a semi-permeable transparent dressing. In the second model, the wound was left uncovered. In both models, the mice were randomly assigned to two treatment groups: saline or PNU282987 (25 mice in each group). In covered wounds, we found that α7-nAChR activation inhibited re-epithelialization, angiogenesis, and epithelial cells proliferation, promoted neo-epithelial detachment, and suppressed neutrophil infiltration and the expression of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF). However, in uncovered wounds, we observed that α7-nAChR activation promoted re-epithelialization and angiogenesis, inhibited neutrophil infiltration and the expression of high mobility group box (HMGB)-1, epidermal growth factor (EGF), and VEGF. In conclusion, this data demonstrated that α7-nAChR activation inhibited wound healing in covered wounds but played an opposite role in uncovered wounds. The opposite effect might be primarily due to inhibition of inflammation.


Subject(s)
Benzamides/pharmacology , Bridged Bicyclo Compounds/pharmacology , alpha7 Nicotinic Acetylcholine Receptor/therapeutic use , Animals , Bandages/adverse effects , Mice , Neovascularization, Physiologic/drug effects , Re-Epithelialization/drug effects , Wound Healing/drug effects , alpha7 Nicotinic Acetylcholine Receptor/agonists
16.
J Opt Soc Am A Opt Image Sci Vis ; 34(8): 1463-1468, 2017 Aug 01.
Article in English | MEDLINE | ID: mdl-29036113

ABSTRACT

A model of anisotropic quasi-homogeneous media with adjustable boundaries is presented with the help of the three-dimensional multi-Gaussian function. After that, the behaviors of the far-zone field of light waves on scattering from an anisotropic semisoft-boundary quasi-homogeneous medium are illustrated. It is shown that the spectral density of the scattered field is flattened-Gaussian-centered, owing to the semisoft boundary of the scatterer, while the spectral degree of coherence is Gaussian-centered, which is independent of the boundary of scatterer. It is also shown that both the spectral density and the spectral degree of coherence are anisotropic because of the anisotropy of the scattering medium.

17.
J Opt Soc Am A Opt Image Sci Vis ; 33(4): 625-9, 2016 04 01.
Article in English | MEDLINE | ID: mdl-27140772

ABSTRACT

Spectral shifts and spectral switches of a polychromatic electromagnetic light wave on scattering from an anisotropic semisoft boundary medium are discussed. It is shown that both the property of the incident field and the character of the scattering medium play roles in the change of the spectrum of the far-zone scattered field. It is also shown that the distribution of the far-zone scattered spectrum, including the magnitude of the spectral shift and the direction at which the spectral switch occurs, is rotationally nonsymmetric.

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