ABSTRACT
Objective: To explore the impact of non-valvular atrial fibrillation (AF) on the global cognitive function and executive function of patients without dementia, and to observe the differences between different types of AF. Methods: This research is a prospective and cross-sectional study. Non-dementia patients admitted to the department of neurology in the third people's hospital of Chengdu from July 2018 to July 2019 were included. Patients with non-valvular AF were included in the AF group and those with sinus rhythm were included in the control group. General clinical data and compared global cognitive function (mini-mental state examination (MMSE) and montreal cognitive assessment (MOCA)) and executive function (shape trails test (STT) and stroop color and word test (SCWT)) data were obtained and compared between 2 groups, and between different AF type groups. Results: A total of 386 participants were included, including 203 in AF group (52.6%), age was 68 (63, 71) years old, 119 were male (58.6%) and 183 in control group, age was 68 (63, 71) years old, 101 were male (55.2%). MMSE(28 (27, 29)) and MOCA (25 (22, 26)) scores were lower in AF group than those in control group (P<0.05), while STT-A time (84 (64, 140) s), STT-B time (248 (184, 351) s), STT time difference((159 (106, 245) s), SCWT-A time (50 (50, 50) s), SCWT-B time (55 (46, 63) s), SCWT-C time (100 (86, 120) s) and SCWT time interference (46 (34, 65) s) were higher than those in control group (P<0.05). Moreover, there was no difference in above indexes between paroxysmal AF and non-paroxysmal AF. Conclusion: The global cognitive function and executive function of patients with non-valvular AF are both decreased, while there is no obvious difference of the global cognitive function and executive function between paroxysmal AF and non-paroxysmal AF patients.
Subject(s)
Humans , Male , Female , Atrial Fibrillation/diagnosis , Executive Function , Prospective Studies , Cross-Sectional Studies , Cognition Disorders/diagnosis , CognitionABSTRACT
In the present study, the complete mitochondrial genome of Trachypithecus francoisi was determined using PCR reactions. The mitochondrial genome is 16,544 bp in length, consisting of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. The structural organization and gene order of T. francoisi were equivalent to that of most other vertebrates. The T. francoisi mtDNA could provide useful data for further studies on phylogenetics and conservation genetics of this species.
Subject(s)
Colobinae/genetics , DNA, Mitochondrial/genetics , Genome, Mitochondrial , Animals , Base Sequence , Phylogeny , Polymerase Chain Reaction , Sequence Analysis, DNA/veterinaryABSTRACT
In this study, the complete mitochondrial genome of Trachypithecus cristatus was determined using PCR method. The mitochondrial genome is 16,557 bp in length, it containing 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. The structural organization and gene order of T. cristatus mtDNA were similar to that of most other vertebrates. The results could provide useful information for further studies on phylogenetics of T. cristatus.
Subject(s)
Colobinae/genetics , Genes, Mitochondrial/physiology , Genome, Mitochondrial/physiology , Animals , Base Sequence , DNA, Mitochondrial/genetics , Mitochondrial Proteins/genetics , Molecular Sequence Data , RNA/genetics , RNA, Mitochondrial , RNA, Ribosomal/genetics , RNA, Transfer/geneticsABSTRACT
To investigate the functions of U6 and 7SK of Bama mini-pig and produce Bama mini-pig with silenced GGTA1 gene, the siRNA promoters U6 and 7SK were cloned, ligated into pMD18-shEGFP, and co-transfected with PEGFP- N1 into PK-15 kidney cells of pigs to be used in RNAi experiments. The functions of the two promoters in pig cells were verified using pMD18-hU6-shEGFP as the positive control, pMD18-shEGFP vector without promoter as the negative control, PEGFP-N1 as the first blank control, ddH2O in replacement of the plasmid as the second blank control. The results showed that the lengths of U6 and 7SK in Bama mini-pig were 553 bp and 437 bp, respectively. Vectors pMD18-pU6- shEGFP and pMD18-p7SK-shEGFP were constructed and transfected into PK-15 cells from pigs. Promoters pU6 and p7SK proved to express high levels of siRNA activity and can be used in the experiment of silencing α-1,3galactosyltransferase gene.
Subject(s)
Promoter Regions, Genetic/physiology , RNA Polymerase III/genetics , RNA, Small Interfering/genetics , Swine, Miniature/genetics , Animals , Base Sequence , Cloning, Molecular , Molecular Sequence Data , Sequence Analysis, DNA , SwineABSTRACT
The mitochondrial COI gene was PCR amplified and sequenced from 17 samples obtained from three populations of Macrobrachium rosenbergii (F1 of the Burma wildtype population, Jiangsu cultured population and F2 of the Guangxi breeding population). A 498-bp long partial gene segment was acquired and used to study the genetic diversity among the three populations. Results indicated that the COI gene locus was relatively more polymorphic in the F1 of Burma wildtype population, while the polymorphism in Jiangsu cultured population and Guangxi breeding population were very poor. A total of 10 polymorphic sites and 5 haplotypes were found in the sequences of the 17 samples. The average nucleotide divergence in the three populations was 0.88%0.07% and 0 respectively. The UPGMA phylogenetic tree suggested that F2 of the Guangxi breeding population and Jiangsu cultured population were closest genetically, and their haplotypes could be gathered together to a genetic branch while F1 of the Burma wildtype population diverged and could form another relatively independent branch. For these distinct nucleotide differences, COI gene could be suitable as a genetic marker for distinguishing these two branches of the Macrobrachium rosenbergii population.
