A structure-functionality insight into the bioactivity of microbial polysaccharides toward biomedical applications: A review.

Microbial polysaccharides (MPs) are biopolymers secreted by microorganisms such as bacteria and fungi during their metabolic processes. Compared to polysaccharides derived from plants and animals, MPs have advantages such as wide sources, high production efficiency, and less susceptibility to natural environmental influences. The most attractive feature of MPs lies in their diverse biological activities, such as antioxidative, anti-tumor, antibacterial, and immunomodulatory activities, which have demonstrated immense potential for applications in functional foods, cosmetics, and biomedicine. These bioactivities are precisely regulated by their sophisticated molecular structure. However, the mechanisms underlying this precise regulation are not yet fully understood and continue to evolve. This article presents a comprehensive review of the most representative species of MPs, including their fermentation and purification processes and their biomedical applications in recent years. In particular, this work presents an in-depth analysis into the structure-activity relationships of MPs across multiple molecular levels. Additionally, this review discusses the challenges and prospects of investigating the structure-activity relationships, providing valuable insights into the broad and high-value utilization of MPs.

Exploring the potential of selective oxidation in bioconjugation of collagen with xyloglucan carboxylates.

Xyloglucan (XG), as a natural biopolymer, possesses a sound biocompatibility and an impressive biodegradability, which are usually featured with abundant hydroxyl groups available for the bioconjugation with a bioactive moiety, suggesting a promising or unique value possibly applied in the field of biomedicine. In this study, XG was extracted from Tamarind seeds and subjected to four regioselective oxidation methods to introduce carboxyl groups onto the XG molecules for a bioconjugation with collagen. Galactose oxidase and reducing end aldehyde group oxidation mainly resulted in a low carboxylate content at ∼0.34 mmol/g, whereas the primary and secondary hydroxyl group oxidations would lead to a high carboxyl content at ∼0.84 mmol/g. The number-average molar mass (Mn) and weight-average molar mass (Mw) of XG were 8.8 × 105 g/mol and 1.1 × 106 g/mol, respectively. The oxidized XGs were then subjected to a further biofunctionalization with the collagen through EDC/NHS coupling, which exhibited a degree of conjugation rate, ranged from 50 % to 72 %. The collagen-conjugated at the C6 position of XGs exhibited the highest cell viability recorded at 168 % in promoting cell growth and proliferation after 72 h of culture, surpassing that of pure collagen recorded at 138 %, which may indeed suggest a promising value in a biomedical application.

Biosafety consideration of nanocellulose in biomedical applications: A review.

Nanocellulose-based biomaterials have gained significant attention in various fields, especially in medical and pharmaceutical areas, due to their unique properties, including non-toxicity, high specific surface area, biodegradability, biocompatibility, and abundant feasible and sophisticated strategies for functional modification. The biosafety of nanocellulose itself is a prerequisite to ensure the safe and effective application of biomaterials as they interact with living cells, tissues, and organs at the nanoscale. Potential residual endogenous impurities and exogenous contaminants could lead to the failure of the intended functionalities or even serious health complications if they are not adequately removed and assessed before use. This review summarizes the sources of impurities in nanocellulose that may pose potential hazards to their biosafety, including endogenous impurities that co-exist in the cellulosic raw materials themselves and exogenous contaminants caused by external exposure. Strategies to reduce or completely remove these impurities are outlined and classified as chemical, physical, biological, and combined methods. Additionally, key points that require careful consideration in the interpretation of the biosafety evaluation outcomes were discussed to ensure the safety and effectiveness of the nanocellulose-based biomaterials in medical applications.

A critical review on plastic waste life cycle assessment and management: Challenges, research gaps, and future perspectives.

The global production and consumption of plastics, as well as their deposition in the environment, are experiencing exponential growth. In addition, mismanaged plastic waste (PW) losses into drainage channels are a growing source of microplastic (MP) pollution concern. However, the complete understanding of their environmental implications throughout their life cycle is yet to be fully understood. Determining the potential extent to which MPs contribute to overall ecotoxicity is possible through the monitoring of PW release and MP removal during remediation. Life cycle assessments (LCAs) have been extensively utilized in many comparative analyses, such as comparing petroleum-based plastics with biomass and single-use plastics with multi-use alternatives. These assessments typically yield unexpected or paradoxical results. Nevertheless, there is still a paucity of reliable data and tools for conducting LCAs on plastics. On the other hand, the release and impact of MP have so far not been considered in LCA studies. This is due to the absence of inventory-related data regarding MP releases and the characterization factors necessary to quantify the effects of MP. Therefore, this review paper conducts a comprehensive literature review in order to assess the current state of knowledge and data regarding the environmental impacts that occur throughout the life cycle of plastics, along with strategies for plastic management through LCA.

Recent advances in sustainable hydrogen production from microalgae: Mechanisms, challenges, and future perspectives.

The depletion of fossil fuel reserves has resulted from their application in the industrial and energy sectors. As a result, substantial efforts have been dedicated to fostering the shift from fossil fuels to renewable energy sources via technological advancements in industrial processes. Microalgae can be used to produce biofuels such as biodiesel, hydrogen, and bioethanol. Microalgae are particularly suitable for hydrogen production due to their rapid growth rate, ability to thrive in diverse habitats, ability to resolve conflicts between fuel and food production, and capacity to capture and utilize atmospheric carbon dioxide. Therefore, microalgae-based biohydrogen production has attracted significant attention as a clean and sustainable fuel to achieve carbon neutrality and sustainability in nature. To this end, the review paper emphasizes recent information related to microalgae-based biohydrogen production, mechanisms of sustainable hydrogen production, factors affecting biohydrogen production by microalgae, bioreactor design and hydrogen production, advanced strategies to improve efficiency of biohydrogen production by microalgae, along with bottlenecks and perspectives to overcome the challenges. This review aims to collate advances and new knowledge emerged in recent years for microalgae-based biohydrogen production and promote the adoption of biohydrogen as an alternative to conventional hydrocarbon biofuels, thereby expediting the carbon neutrality target that is most advantageous to the environment.

Biodegradation of willow sawdust by novel cellulase-producing bacterial consortium from wood-feeding termites for enhancing methane production.

This study was designed to develop a cellulase-producing bacterial consortium (CBC) from wood-feeding termites that could effectively degrade willow sawdust (WSD) and consequently enhance methane production. The bacterial strains Shewanella sp. SSA-1557, Bacillus cereus SSA-1558, and Pseudomonas mosselii SSA-1568 exhibited significant cellulolytic activity. Their CBC consortium showed positive effects on cellulose bioconversion, resulting in accelerated WSD degradation. After nine days of pretreatment, the WSD had lost 63%, 50%, and 28% of its cellulose, hemicellulose, and lignin, respectively. The hydrolysis rate of treated WSD (352 mg/g) was much higher than that of untreated WSD (15.2 mg/g). The highest biogas production (66.1 NL/kg VS) with 66% methane was observed in the anaerobic digester M-2, which contained a combination of pretreated WSD and cattle dung in a 50/50 ratio. The findings will enrich knowledge for the development of cellulolytic bacterial consortia from termite guts for biological wood pretreatment in lignocellulosic anaerobic digestion biorefineries.

Fabrication of bio-inspired anisotropic structures from biopolymers for biomedical applications: A review.

The anisotropic features play indispensable roles in regulating various life activities in different organisms. Increasing efforts have been made to learn and mimic various tissues' intrinsic anisotropic structure or functionality for broad applications in different areas, especially in biomedicine and pharmacy. This paper discusses the strategies for fabricating biomaterials using biopolymers for biomedical applications with the case study analysis. Biopolymers, including different polysaccharides, proteins, and their derivates, that have been confirmed with sound biocompatibility for different biomedical applications are summarized, with a special focus on nanocellulose. Advanced analytical techniques for understanding and characterizing the biopolymer-based anisotropic structures for various biomedical applications are also summarized. Challenges still exist in precisely constructing biopolymers-based biomaterials with anisotropic structures from molecular to macroscopic levels and fitting the dynamic processes in native tissue. It is foreseeable that with the advancement of biopolymers' molecular functionalization, biopolymer building block orientation manipulation strategies, and structural characterization techniques, developing anisotropic biopolymer-based biomaterials for different biomedical applications would significantly contribute to a friendly disease-curing and healthcare experience.

Environmental and Human Health Impact of Disposable Face Masks During the COVID-19 Pandemic: Wood-Feeding Termites as a Model for Plastic Biodegradation.

The ongoing COVID-19 pandemic has resulted in an unprecedented form of plastic pollution: personal protective equipment (PPE). On the eve of the COVID-19 pandemic, there is a tremendous increase in the production of plastic-based PPE. To control the spread of the virus, face masks (FMs) are used as primary PPE. Thus, the production and usage of FM significantly increased as the COVID-19 pandemic was still escalating. The primary raw materials for the manufacturing of FMs are non-biodegradable synthetic polymers derived from petrochemicals. This calls for an urgent need to develop novel strategies for the efficient degradation of plastics. Furthermore, most of these masks contain plastic or other derivatives of plastic. The extensive usage of FM generates millions of tons of plastic waste for the environment in a short span of time. However, their degradation in the environment and consequences are poorly understood. Therefore, the potential impacts of disposable FM on the environment and human health during the COVID-19 pandemic are clarified in the present study. Despite structural and recalcitrance variations, lignocellulose and plastic polymers have physicochemical features, including carbon skeletons with comparable chemical bonds as well as hydrophobic properties in amorphous and crystalline regions. In this review, we argue that there is much to be learned from termites by transferring knowledge from research on lignocellulose degradation by termites to that on plastic waste.

Functionalization of nanocellulose applied with biological molecules for biomedical application: A review.

Nanocellulose has great potential in the biomedical field due to its biocompatibility, large specific surface area, and customizable surface chemistry. However, due to the bioinert nature and mismatch of the mechanical strength, nanocellulose itself has no cell adhesion ability and cannot directly promote cell growth and reproduction. Recently, surface functionalization of nanocellulose has been reported as an indispensable strategy for improving its bioactivities or other physic-chemical properties. In this paper, functionalization strategies of nanocellulose based on its inherent hydroxyl, aldehyde, carboxyl, and sulfate group reactions are reviewed. Biomacromolecules, such as peptides, proteins, and DNA that are commonly used in functionalization for different biomedical applications are summarized. Prospects and ongoing challenges of nanocellulose-based biomaterials application, as well as these advanced processing technologies such as additive manufacturing, nanomanufacturing, and bio-manufacturing are also discussed. This review is supposed to serve as a guideline for the development of nanocellulose-based biomaterials in biomedical applications.

Development of Cellulose Nanofibril/Casein-Based 3D Composite Hemostasis Scaffold for Potential Wound-Healing Application.

Excessive bleeding in traumatic hemorrhage is the primary concern for natural wound healing and the main reason for trauma deaths. The three-dimensional (3D) bioprinting of bioinks offers the desired structural complexity vital for hemostasis activity and targeted cell proliferation in rapid and controlled wound healing. However, it is challenging to develop suitable bioinks to fabricate specific 3D scaffolds desirable in wound healing. In this work, a 3D composite scaffold is designed using bioprinting technology and synergistic hemostasis mechanisms of cellulose nanofibrils (TCNFs), chitosan, and casein to control blood loss in traumatic hemorrhage. Bioinks that consist of casein bioconjugated TCNF (with a casein content of 104.5 ± 34.1 mg/g) using the carbodiimide cross-linker chemistry were subjected to bioprinting for customizable 3D scaffold fabrication. Further, the 3D composite scaffolds were in situ cross-linked using a green ionic complexation approach. The covalent conjugation among TCNF, casein, and chitosan was confirmed by Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), X-ray photoelectron spectroscopy (XPS), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and X-ray diffraction (XRD) studies. The in vitro hemostasis activity of the 3D composite scaffold was analyzed by a human thrombin-antithrombin (TAT) assay and adsorption of red blood cells (RBCs) and platelets. The 3D composite scaffold had a better swelling behavior and a faster whole blood clotting rate at each time point than the 3D TCNF scaffold and commercial cellulose-based dressings. The TAT assay demonstrated that the 3D composite scaffold could form a higher content of thrombin (663.29 pg/mL) and stable blood clot compared to a cellulosic pad (580.35 pg/mL), 3D TCNF (457.78 pg/mL), and cellulosic gauze (328.92 pg/mL), which are essential for faster blood coagulation. In addition, the 3D composite scaffold had a lower blood clotting index (23.34%) than the 3D TCNF scaffold (41.93%), suggesting higher efficiencies for RBC entrapping to induce blood clotting. The in vivo cytocompatibility was evaluated by a 3D cell culture study, and results showed that the 3D composite scaffold could promote growth and proliferation of NIH 3T3 fibroblast cells, which is vital for wound healing. Cellulase-based in vitro deconstruction of the 3D composite scaffold showed significant weight loss (80 ± 5%) compared to the lysozyme hydrolysis (22 ± 5%) after 28 days of incubation, suggesting the biodegradation potential of the composite scaffold. In conclusion, this study proposes efficient prospects to develop a 3D composite scaffold from bioprinting of TCNF-based bioinks that can accelerate blood clotting and wound healing, suggesting its potential application in reducing blood loss during traumatic hemorrhage.

A critical review on the treatment of dye-containing wastewater: Ecotoxicological and health concerns of textile dyes and possible remediation approaches for environmental safety.

The synthetic dyes used in the textile industry pollute a large amount of water. Textile dyes do not bind tightly to the fabric and are discharged as effluent into the aquatic environment. As a result, the continuous discharge of wastewater from a large number of textile industries without prior treatment has significant negative consequences on the environment and human health. Textile dyes contaminate aquatic habitats and have the potential to be toxic to aquatic organisms, which may enter the food chain. This review will discuss the effects of textile dyes on water bodies, aquatic flora, and human health. Textile dyes degrade the esthetic quality of bodies of water by increasing biochemical and chemical oxygen demand, impairing photosynthesis, inhibiting plant growth, entering the food chain, providing recalcitrance and bioaccumulation, and potentially promoting toxicity, mutagenicity, and carcinogenicity. Therefore, dye-containing wastewater should be effectively treated using eco-friendly technologies to avoid negative effects on the environment, human health, and natural water resources. This review compares the most recent technologies which are commonly used to remove dye from textile wastewater, with a focus on the advantages and drawbacks of these various approaches. This review is expected to spark great interest among the research community who wish to combat the widespread risk of toxic organic pollutants generated by the textile industries.

Construction of a novel microbial consortium valued for the effective degradation and detoxification of creosote-treated sawdust along with enhanced methane production.

Lignocellulosic biomass represents an unlimited and ubiquitous energy source, which can effectively address current global challenges, including climate change, greenhouse gas emissions, and increased energy demand. However, lignocellulose recalcitrance hinders microbial degradation, especially in case of contaminated materials such as creosote (CRO)-treated wood, which necessitates appropriate processing in order to eliminate pollution. This study might be the first to explore a novel bacterial consortium SST-4, for decomposing birchwood sawdust, capable of concurrently degrading lignocellulose and CRO compounds. Afterwards, SST-4 which stands for molecularly identified bacterial strains Acinetobacter calcoaceticus BSW-11, Shewanella putrefaciens BSW-18, Bacillus cereus BSW-23, and Novosphingobium taihuense BSW-25 was evaluated in terms of biological sawdust pre-treatment, resulting in effective lignocellulose degradation and 100% removal of phenol and naphthalene. Subsequently, the maximum biogas production observed was 18.7 L/kg VS, while cumulative methane production was 162.8 L/kg VS, compared to 88.5 without microbial pre-treatment. The cumulative energy production from AD-I and AD-II through biomethanation was calculated as 3177.1 and 5843.6 KJ/kg, respectively. The pretreatment process exhibited a significant increase in the energy yield by 83.9%. Lastly, effective CRO detoxification was achieved with EC50 values exceeding 90%, showing the potential for an integrated process of effective contaminated wood management and bioenergy production.

Nanobiotechnological advancements in agriculture and food industry: Applications, nanotoxicity, and future perspectives.

The high demand for sufficient and safe food, and continuous damage of environment by conventional agriculture are major challenges facing the globe. The necessity of smart alternatives and more sustainable practices in food production is crucial to confront the steady increase in human population and careless depletion of global resources. Nanotechnology implementation in agriculture offers smart delivery systems of nutrients, pesticides, and genetic materials for enhanced soil fertility and protection, along with improved traits for better stress tolerance. Additionally, nano-based sensors are the ideal approach towards precision farming for monitoring all factors that impact on agricultural productivity. Furthermore, nanotechnology can play a significant role in post-harvest food processing and packaging to reduce food contamination and wastage. In this review, nanotechnology applications in the agriculture and food sector are reviewed. Implementations of nanotechnology in agriculture have included nano- remediation of wastewater for land irrigation, nanofertilizers, nanopesticides, and nanosensors, while the beneficial effects of nanomaterials (NMs) in promoting genetic traits, germination, and stress tolerance of plants are discussed. Furthermore, the article highlights the efficiency of nanoparticles (NPs) and nanozymes in food processing and packaging. To this end, the potential risks and impacts of NMs on soil, plants, and human tissues and organs are emphasized in order to unravel the complex bio-nano interactions. Finally, the strengths, weaknesses, opportunities, and threats of nanotechnology are evaluated and discussed to provide a broad and clear view of the nanotechnology potentials, as well as future directions for nano-based agri-food applications towards sustainability.