ABSTRACT
In this work, a novel sensitive electrochemiluminescence immunosensor based on Ru@SiO2-Au NPs and Co(OH)2 two-dimensional nanosheets (2D Co(OH)2) is constructed for the detection of enrofloxacin (ENR). Ruthenium bipyridine silica spheres and modified gold nanoparticles were synthesized as immune probe materials, which were combined with ENR antibodies (Abs) to form the immune probe part. 2D Co(OH)2 with a large specific surface area and good catalytic effect was firstly used as an immune substrate material, and at the same time, it was conjugated with the coating antigen (Ae) of ENR to form an immune substrate. Based on the principle of competitive immunity, ENR and ENR coated antigen could jointly compete for the specific binding sites on the ENR antibody, so as to achieve efficient detection of ENR. Under optimal conditions, the prepared immunosensor exhibited high sensitivity with a wide linear range from 0.0001 to 1000 ng mL-1 and a low detection limit (LOD) of 0.063 pg mL-1. The proposed immunosensor has been successfully applied to the detection of ENR residues in poultry, aquatic products and lake water.
Subject(s)
Biosensing Techniques , Metal Nanoparticles , Electrochemical Techniques , Enrofloxacin , Gold , Immunoassay , Limit of Detection , Luminescent Measurements , Silicon DioxideABSTRACT
Invited for the cover of this issue is X. Zeng and co-workers at Soochow University, University of Stuttgart, and Max-Planck Institute for Solid State Research. The image depicts the fast tunneling transformation of the highly elusive metaphosphorous acid (HOPO). Read the full text of the article at 10.1002/chem.202000844.
ABSTRACT
Metaphosphorous acid (HOPO), a key intermediate in phosphorus chemistry, has been generated in syn- and anti-conformations in the gas phase by high-vacuum flash pyrolysis (HVFP) of a molecular precursor ethoxyphosphinidene oxide (EtOPOâC2 H4 +HOPO) at ca. 1000â K and subsequently trapped in an N2 -matrix at 2.8â K. Unlike the two conformers of the nitrogen analogue HONO, the anti-conformer of HOPO undergoes spontaneous rotamerization at 2.8â K via hydrogen-atom tunneling (HAT) with noticeable kinetic isotope effects for H/D (>104 for DOPO) and 16 O/18 O (1.19 for H18 OPO and 1.06 for HOP18 O) in N2 -matrices.
