Elucidating carbon conversion and bacterial succession by amending Fenon-like systems during co-composting of pig manure and branch.

The essential point of current study was to investigate the effect of a Fenton-like system established by oxalic acid and Fe(II) on gas emission, organic matter decomposition and humification during composting. Branches were pretreated with Fenton reagents (0.02 M FeCl2·4H2O + 1.5 M H2O2) and then adding 10 % oxalic acid (OA). The treatments were marked as B1 (control), B2 (Fenton reagent), B3 (10% OA) and B4 (Fenton-like reagent). The results collected from 80 d of composting showed that adding Fenton-like reagent benefited the degradation of organic substances, as reflected by the total organic carbon and dissolved organic carbon, and the maximum decomposition rate was observed in B4. In addition, the Fenton-like reagent could improve the synthesis of humus characterized by complex and stable compounds, which was consistent with the spectral parameters (SUVA254, SUVA280, E253/E203 and Fourier transform-infrared indicators) of DOC. Furthermore, the functional microbial succession performance and linear discriminant effect size analyses provided microbial evidence of humification improvement. Notably, compared with the control, the minimum value of CH4 cumulation was reported in B4, which decreased by 30.44 %. Concluded together, the addition of a Fenton-like reagent composed by OA and Fe(II) is a practical way to improve the humification. Furthermore, the mechanisms related to the promotion of humification should be investigated from free radicals, functional genes, and metabolic pathways.

Humification and fungal community succession during pig manure composting: Membrane covering and mature compost addition.

The objective of this study was to elucidate the combined effect of a semi-permeable membrane (M) and mature compost (MC) on humification and fungal community succession in pig manure composting. Compared with the control, the concentrations of humic substances (HSs) increased by 44.54 % (M + 15 % MC) and 43.90 % (M). During the thermophilic phase, Aspergillus (67.26 %) was the dominant genus in the M + 15 % MC treatment. Membrane covering increased the relative abundance (RA) of other phyla (except for Ascomycetes and Basidiomycetes) on the 14th day and Basidiomycetes on the 80th day in M treatment. Humic acid, HSs were positively correlated with the RA of genera Myceliophthora, Kernia, and Mycothermus. Myceliophthora was the key genus in the M + 15 % MC treatment on the 80th day. The results showed that 15 % MC addition under membrane covering optimizes the quality of composting products.

The efficient solution to decline the greenhouses emission and enrich the bacterial community during pig manure composting: Regulating the particle size of cornstalk.

In present study, four lengths of chopped cornstalks were amended with pig manure respectively for 100 days aerobic fermentation, which aimed to evaluate the impact of different length of agricultural solid wastes on gaseous emission and dominating bacterial community succession and connection. The result revealed that the maximum ammonia volatilization was observed in 5 cm of straw samples attributed to the prominent mineralization, which was opposite to the emission of CH4 and N2O. As for global warming potential, the minimum value was detected in 5 cm of straw samples, which decreased by 5.03-24.75% compared with other samples. Additionally, the strongest correlation and complexity of bacterial community could be detected in 5 cm of straw treatment, representing the most vigorous bacterial metabolic ability could be recorded by optimizing the microbial habitat. Therefore, in order to decline the greenhouse effect in livestock manure composting, the 5 cm of corn straw was recommended.

Exploring gaseous emissions and pivotal enzymatic activity during co-composting of branch and pig manure: The effect of particle size of bulking agents.

The purpose of current study was to probe the effect of various length of branch on gaseous emissions and vital enzymatic activity. Four lengths (< 2 cm, 2 cm, 5 cm, and > 5 cm) of clipped branch were mingled with collected pig manure for 100 days aerobic fermentation. The consequence demonstrated that the amendment of 2 cm of branch showed conducive to decline the greenhouse gas emissions, which the CH4 emissions decreased by 1.62-40.10%, and the N2O emissions decreased by 21.91-34.04% contrasted with other treatments. Furthermore, the peak degree of enzymatic activities was also observed in 2 cm of branch treatment by the optimizing living condition for microbes. In view of microbiological indicators, the most abundant and complex bacterial community could be monitor in 2 cm of branch composting pile, which verified the microbial facilitation. Summing up, the strategy of 2 cm branch amendment would be recommended.

Humification improvement by optimizing particle size of bulking agent and relevant mechanisms during swine manure composting.

For purpose of clarifying the impact on particle size of bulking agents on humification and relevant mechanisms, different length (<2 cm, 2 cm, 5 cm, 10 cm) of branch and straw were blended with swine manure individually for 100 days aerobic composting. Results demonstrated that, 2 cm and 5 cm of branch and straw promoted the highest degradation of DOC by 41.49 % and 58.42 %, and increased the humic substances by 23.81 % and 55.82 % in maturity stage, respectively, compared with other treatments. As shown in microbial consequence, the maximum relative abundance of humus funguses increased by 99.55 % and 99.92 % at phylum, and 98.95 % and 99.24 % at genus in 2 cm and 5 cm of branch and straw treatment, thus verifying the result in variation of humus content. In a word, particle size could result in obvious impact on humification, and the optimized size were about 2 cm and 5 cm of branch and straw.

Magnesite driven the complementary effects of core fungi by optimizing the physicochemical parameters in pig manure composting.

The effects of magnesite (MS) on fungi communities and the core fungi complementarity during pig manure (PM) composting were explored. Different dosage of MS [0% (T1), 2.5% (T2), 5% (T3), 7.5% (T4) and 10% (T5)] as amendments mixed with PM for 42 days composting. The results showed the dominant of phyla were Ascomycota (78.87%), Neocallimastigomycota (41.40%), Basidiomycota (30.81%) and Aphelidiomycota (29.44%). From day 7 to 42, the abundance of Ascomycota and Aphelidiomycota were increased from 7.75% to 42.41% to 57.27%-78.87% and 0-0.70% to 11.73%-29.44% among all treatments. Nevertheless, the phyla abundance of Neocallimastigomycota and Basidiomycota decreased from day 7 to 42. The co-occurrence network indicated that the high additive amendment could enhance the core fungi complementarity effects capacity. The 10% MS addition was a promisable candidate to optimum fungal communities, and causing a better compost quality. This study illustrated the potential and fungi communities changing of MS as additives in composting.

Effects of the triple therapy of carnosine glycoside, edaravone, and Xueshuantong in hemorrhagic cerebral infarction.

OBJECTIVE: This study was designed to evaluate the effects of the triple therapy of Muscular Amino Acid and Peptides and Nucleosides (MAAPN), edaravone, and Xueshuantong on neurological function, tumor volume, and adverse reactions in patients with hemorrhagic cerebral infarction. METHODS: In this retrospective study, a total of 115 patients with hemorrhagic cerebral infarction admitted to the hospital from January 2020 to January 2021 were enrolled and assigned to the observation group (n=57) or the control group (n=58) according to different treatment methods. The two groups were both treated with a conventional treatment regimen, and the observation group was additionally given carnosine, edaravone, and Xueshuantong, with a course of treatment spanning 14 days. The neurological and motor functions and changes in cerebral edema and cerebral infarct lesion size in patients were evaluated. The levels of inflammatory factors, blood lipids, neuron-specific enolase (NSE), S-100ß, and matrix metalloproteinase-9 (MMP-9) of the two groups were determined and compared. The adverse effects and rebleeding of patients were recorded. The Barthel index (BI) was used to evaluate the quality of life of patients. RESULTS: The treatment efficiency in the observation group was significantly higher than that in the control group (P<0.05). After treatment, the observation group obtained more favorable outcomes in terms of the neurological and motor functions, lesions of brain edema and cerebral infarction, and BI scores, than those of the control group (all P<0.05). In addition, after treatment, the levels of inflammatory factors, blood lipids, NSE, S-100ß, MMP-9, plasma viscosity, and whole blood viscosity of the two groups of patients all decreased remarkably, with better outcomes in the observation group when compared with the control group (all P<0.05). The observation group showed a markedly lower rebleeding rate than the control group (P<0.05). CONCLUSION: For patients with hemorrhagic cerebral infarction, the triple therapy of carnosine glycoside, edaravone, and Xueshuantong effectively enhances the neurological and motor function, reduces cerebral edema and cerebral infarction, and improves the quality of life, with high safety.

Effects of further composting black soldier fly larvae manure on toxic metals and resistant bacteria communities by cornstalk amendment.

Rapid composting by black soldier fly larvae (BSFL) may be insufficient to maturation and humification of composting and further composting is necessary. The purpose of this study was to explore cornstalk addition on toxic metals (Cu, Zn, Pb and Cd), toxic metals resistance bacterial (TMRB) destiny and their relationship with physicochemical factors during BSFL manure composting. High-throughput sequencing was performed by six treatments, namely T1 to T6, where T1 to T3 were BSFL manures from chicken, pig and dairy manure, respectively, and T4 to T6 were same manures and utilized cornstalk to adjust C/N to 25. The results showed that cornstalk amendment could enhance the toxic metals immobilization rate compared to control treatments in the ultimate product. TMRB indicated that the major potential hosts bacteria were Firmicutes, Bacteroidota, Proteobacteria, Acidobacteriota and Actinobacteriota, and the sum relative abundance were 63.33%, 90.62%, 83.62%, 69.38%, 50.66% and 90.52% in T1 to T6 at the end of composting. Bacteria diversity and heat map revealed composting micro-ecology with additive cornstalk to remarkably effect main resistant bacterial distribution via adjusting environmental factors and potential hosts bacterial. Finally, T5 treatment was able to greatly decrease the TMRB abundance, and improve the ability of composting and ultimate product quality.

Potential of Forsythoside I as a therapeutic approach for acute lung injury: Involvement of TXNIP/NLRP3 inflammasome.

OBJECTIVE: To explore the role of Forsythoside I (FI) in acute lung injury (ALI) mouse and its underling mechanism. METHODS: The cell models of ALI are constructed by LPS induction. After pretreatment with different concentrations of FI, the lung injury is assessed by pathological changes of lung tissues and cell apoptosis. The cell viability, levels of pro-inflammatory cytokines, and the activation of TXNIP/NLRP3 pathway are inspected to investigate whether the effect of FI on inflammatory response is exerted by regulating the TXNIP/NLRP3 pathway. RESULTS: LPS induces inflammatory cell infiltration, tissue necrosis and pulmonary interstitial edema of mouse tissues, and LPS increases the protein concentration and levels of pro-inflammatory factors in mouse BALF. Additionally, enhanced cell apoptotic level, increased W/D ratio and MPO activity, as well as suppressed SOD activity are observed in LPS-induced mouse models. Those inflammation response, oxidative stress and lung injury can be attenuated by FI (12.5 mg/kg, 25 mg/kg, 50 mg/kg) in a dose-dependent manner. Meanwhile, both in vitro and in vivo studies reveal that FI can lead to suppressed TXNIP expression and inactivated NLRP3 inflammasomes. TXNIP is an upstream target of NLRP3, and FI mitigates ALI by decreasing TXNIP to block NLRP3 inflammasomes. CONCLUSION: FI protects against ALI through the mediation of TXNIP/NLRP3 inflammasome axis and therefore has a certain potential for ALI treatment.

Conversion food waste and sawdust into compost employing black soldier fly larvae (diptera: Stratiomyidae) under the optimized condition.

The increasing number of food waste (FW) had led to an urgent methods to recycle, black soldier fly larvae (BSFL) was a potential quick waste manager. To assess the impact of BSFL on conversion FW and sawdust into compost via the parameters of maturity, nutrient transformation and volatile fatty acids (VFAs). Meanwhile, the artificial of FW contained noodles, cabbages, rice and pork. FW and sawdust were employed by BSFL (6.5:0.5:1.2 ratio on fresh weight basis) as T1 and without BSFL called control (T2), while moisture content for FW and sawdust was 86.57% and 5.98%. Results illustrated that BSFL declined the composting time and only 9 d. Compared with initial mixture materials, T1 decreased organic matter, total kjeldahl nitrogen and VFAs from 97.41 to 85.96%, 23.01 to 17.77 g kg-1 and 3.25 to 1.69 g kg-1, respectively. However, T1 increased the total phosphorous and total potassium in 3.8 folds and 5 folds. The value of pH and EC reached at 4.27 and 1100 µS cm-1, and the germination index attained to 70.69%. In addition, redundancy analysis was used to analysis the correlation between factors under composting employed with different additive ratio of BSFL. Therefore, BSFL played a vital role in FW and sawdust recycling, especially reduced composting time and made the final separation of larvae and substrate easily, saving labor costs.

Changes of fungal diversity in fine coal gasification slag amendment pig manure composting.

The purpose of this study was to investigate fungal diversity and relative abundance (RA) during pig manure composting via high-throughput sequencing approach. Fine coal gasification slag (FCGS) (0%, 2%, 4%, 6%, 8% and 10%) were added into composting raw materials as additive and performed 42 days. Adjust C/N and moisture to 30 and 65%. Results showed that dominant phyla were Ascomycota (99.62%) and Basidiomycota (0.38%). The main genera were Epicoccum (1.26%), Alternaria (83.35%), Aspergillus (12.08%) and Gibberella (1.69%). 10% treatment got the higher abundance and operational taxonomic units number from rank abundance curve and petals diagram. Compared with control, FCGS amendment composting could increase the sanitary time (3-7 d) and total nitrogen (0.05-12.03%). The principal component analysis was considered that FCGS treatments and control had significantly difference. The RA of fungi varied among all treatments. Therefore, 10% treatment was a potential candidate to enhance fungal diversity and composting quality.