Establishment of risk stratification model for developing cirrhosis in patients with HBeAg-negative chronic hepatitis B virus infection / 中华检验医学杂志

Objective:To explore the independent predictive factors of cirrhosis in patients with hepatitis B e antigen (HBeAg)-negative chronic hepatitis B virus (HBV) infection, and establish a nomogram model based on clinical laboratory data and analyze the predictive value of this model.Methods:The laboratory data of 596 patients with HBeAg-negative chronic HBV infection and 677 patients with hepatitis B cirrhosis, who were hospitalized in the First Hospital of China Medical University from 2011 to 2021, were retrospectively analyzed. Patients were randomly divided into training group ( n=892) and validation group ( n=381) at the ratio of 7∶3. The independent predictive factors of cirrhosis were analyzed by univariate logistic regression, multiple collinearity test and multivariate logistic regression. The nomogram model was established and the prediction value of this model was evaluated. Results:According to multivariate logistic regression analysis, hepatitis B core antibody ( OR=1.492, 95% CI 1.316-1.706), glutamine transpeptidase ( OR=1.015, 95% CI 1.010-1.022), platelet ( OR=0.986, 95% CI 0.982-0.988) and albumin ( OR=0.853, 95% CI 0.824-0.882) were independent predictors of cirrhosis ( P<0.05), and the nomogram was established based on the four indicators. Receiver operating characteristic curves showed that area under the curve of the nomogram was 0.933 (95% CI 0.916-0.950), and that of the validation group was 0.931 (95% CI 0.905-0.956). The calibration curves indicated the nomogram model was highly consistent with the actual outcome. Decision curves and clinical impact curves confirmed that the model had high net benefit and good clinical application performance. Conclusions:Hepatitis B core antibody, glutamine transpeptidase, platelet and albumin are independent predictors of cirrhosis among patients with HBeAg-negative chronic HBV infection. The newly developed nomogram model based on these factors could be used to predict cirrhosis risk in these patients.

Experimental Study on Separation of Fetal Nucleated Red Blood Cell from the Whole Blood Using Microfluidic Chip / 医用生物力学

Objective To seperate fetal nucleated red blood cells (fNRBCs) from the whole maternal peripheral blood effectively by designing a circular channel microfluidic chip. Methods A microfluidic chip is designed by utilizing the margination in blood flow and the specific adhesion characteristics of immuno-agent anti-CD147. With the whole umbilical cord blood, the effects of different shear forces on the enrichment of fNRBCs was studied by immunofluorescence counting. Results Increasing shear rate in microfluidic chip could improve the number of captured fNRBCs compared with the static adhesion. With the increase of shear rate of blood flow, the number of the captured cells increased at first, and then decreased. Conclusions The use of microfluid chip can effectively seperate fNRBCs from the whole blood. The results provide an experimental reference for the non-invasive prenatal diagnosis research and the exploration on the mechanism of fetal cell migration.

Spontaneous neural activity in different frequency bands in Parkinson 's disease：a fMRI study / 中华行为医学与脑科学杂志

Objective To explore the pattern of spontaneous neural activity in different frequency bands in Parkinson's disease(PD).Methods The blood oxygen level-dependent signals in 39 PD patients and 37 normal controls were acquired and the amplitude of low-frequency fluctuation ( ALFF) in five differ-ent frequecy bands ( slow-2:0.198-0.25 Hz;slow-3:0.073-0.198 Hz;slow-4:0.027-0.073 Hz;slow-5:0.01-0.027 Hz;slow-6:0-0.01Hz) was cacaulated.A repeated-measures analysis of variance and Post hoc t-test were performed to detect differences of spontaneous activity between two groups in different frequency bands. A correlation analysis was examined between Unified Parkinson’ s Disease Rating Scale ( UPDRS) motor score and ALFF in patients with PD in each frequency band to discover the relationship between disease se-verity and spontaneous neural activity.Results Compared with the normal controls,patients with PD had decreased ALFF in the putamen(-24,-3,-9) (-30,3,0) ( -21,9,6) in the slow-3,slow-4 and slow-5, and had decreased ALFF in the left precentral gyrus (-21,-24,75) (-21,-24,72) (-27,-21,72) (-15,-15,72) and right supplementary motor area (21,-24,75) (6,-3,72) (12,-6,60) (15,-24,72) in all frequency bands except for the slow-5.Increased ALFF in the cerebellum was found in all frequency bands.The ALFF in the putamen was negatively correlated with the UPDRS motor score in the slow-3,slow-5 and slow-6.Conclusion This study shows that the abnormal activities in the striatum are significant in the slow-3,slow-4and slow-5.Most frequency bands except slow-5 are helpful in detecting neural changes in mo-tor cortex.The increased activity in the cerebellum is the common findings in all frequency bands.Multiple frequency analysis may provide insight into the pathophysiological mechanisms of PD.

Aire promotes the self-renewal of embryonic stem cells through Lin28.

Abstract Autoimmune regulator (Aire) is one of the most well-characterized molecules in autoimmunity, but its function outside the immune system is largely unknown. The recent discovery of Aire expression in stem cells and early embryonic cells and its function in the self-renewal of embryonic stem (ES) cells highlight the importance of Aire in these cells. In this study, we present evidence that Aire promotes the expression of the pluripotent factor Lin28 and the self-renewal of ES cells. We presented the first evidence that the let-7 microRNA family contributed to the self-renewal promoting effect of Aire on ES cells. Moreover, we showed that Aire and Lin28 are co-expressed in the genital ridge, oocytes, and cleavage-stage embryos, and the expression level of Lin28 is correlated with the expression level of Aire. Although it is widely considered to be a promiscuous gene expression activator, these results indicated that Aire promotes the self-renewal of ES cells through a specific pathway (i.e., the activation of Lin28 and the inhibition of the let-7 microRNA family). The correlation between Aire and Lin28 expression in germ cells and early embryos indicated an in vivo function for Aire in toti- and pluripotent stem cells. This study presents the first molecular pathway that incorporates Aire into the pluripotency network. Moreover, it presents the first evidence that microRNAs contribute to the regulatory function of Aire and highlights a novel function of Aire in stem cell biology and reproduction. These functions reveal novel perspectives for studying the molecular mechanisms behind the establishment and sustenance of pluripotent identity.

Clinical Analysis of Seven Cases with Hemolysis,Elevated Liver Enzymes and Low Platelets Syndrome / 医学研究杂志

Objective To investigate the diagnosis and treatment of HELLP syndrome through analyzing the clinical data of seven patients of HELLP syndrome.Methods We reviewed the study.There were three patients in the absolute HELLP group,four patients in the partial HELLP group.The laboratory data,clinical symptom,delivery method and the outcome of the pregnant women and infants of the two groups were analyzed.Results All the seven patients had clinical presentations of PIH.Total bilirubin(TB) value in absolute HELLP group was significantly higher than that in partial HELLP group(P