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J Appl Microbiol ; 126(3): 864-871, 2019 Mar.
Article in English | MEDLINE | ID: mdl-30520218

ABSTRACT

AIMS: We developed a new rapid and reliable method for identifying bacteria using a combination of Fourier transform infrared (FT-IR) spectroscopy of bacterial genomic DNA and multivariate analysis. METHODS AND RESULTS: FT-IR spectra of genomic DNA from four type strains of Pseudomonas spp., three type strains of Escherichia spp. and two type strains of Bacillus spp. were analysed in the 4000-400 cm-1 region. Spectral differences were found in the frequency regions of N-H stretching (amide I), C=O stretching vibrations (amide II) and PO2 - ionized asymmetric and symmetric stretching. Partial least squares discriminant analysis of the FT-IR spectra showed that the microbial strains could be discriminated by hierarchical clustering analysis. CONCLUSIONS: FT-IR spectral analysis of bacterial genomic DNA has potential for the rapid identification of bacteria at the genus and species levels. SIGNIFICANCE AND IMPACT OF THE STUDY: This study reports a new bacterial identification method using multivariate analysis of FT-IR spectra of bacterial genomic DNA.


Subject(s)
Bacteria , Bacterial Typing Techniques/methods , DNA, Bacterial , Spectroscopy, Fourier Transform Infrared/methods , Bacteria/classification , Bacteria/genetics , DNA, Bacterial/analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics
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