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Objective @#To investigate the effect of ginsenoside Rg1 (GRg1) on human retinal microvascular endothelial cells (HRMECs) glycolysis by regulating pyruvate kinase M2 ( PKM2) expression.@*Methods @#HRMECs were cultured in vitro and divided into normal control (NC) group, high glucose (HG) group, high glucose + ginsenoside Rg1 (HG + GRg1) group, high glucose + ginsenoside Rg1 + low expression PKM2 ( HG + GRg1 + si-PKM2) group, and high glucose + ginsenoside Rg1 + overexpression PKM2 (HG + GRg1 + OE⁃PKM2) group. si-PKM2 and OE⁃PKM2 were transfected into HRMECs cells by cell transfection. The expression of PKM2 mRNA in HRMECs was detected by qRT⁃PCR. The expression levels of related proteins in HRMECs were detected by Western blot. The number of lumen formation in vitro was observed under an inverted microscope to quantify the angiogenesis ability. Cell culture medium of each group was collected, and glucose intake, lactate production and adenosine triphosphate(ATP)content were detected by glucose detection kit, lactate detection kit and ATP detection kit,re spectively. @*Results @#HG induced HRMECs significantly increased the number of blood vessel formation, glycolysis and PKM2 expression, while GRg1 treatment significantly reduced the number of blood vessel formation, glycolysis and PKM2 expression; transfection of si⁃PKM2 assisted the inhibitory effect of GRg1 on glycolysis and angiogenesis while transfection of OE⁃PKM2 interfered with the function of GRg1 . @*Conclusion @#GRg1 inhibits angiogenesis by inhibiting PKM2 to reduce glycolysis of HRMECs.
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BackgroundThe outbreak of coronavirus disease 2019 (COVID-19) in Wuhan City, China has spreads rapidly since December, 2019. Most patients show mild symptoms, but some of them develop into severe disease. There is currently no specific medication. The purpose of this study is to explore changes of markers in peripheral blood of severe COVID-19 patients, which may be of value in disease monitoring. MethodsClinical data of patients with nonsevere and severe type COVID-19 diagnosed by laboratory test in our institution were collected. The relationship between peripheral blood cells and cytokines, clinical manifestation and outcome was analyzed. ResultsA total of 69 severe type COVID-19 patients were included. On admission, the median age of severe cases was 56-year old, with 52.17% female patient. The most common symptoms were fever (79.72%), cough (63.77%), shortness of breath (57.97%) and fatigue (50.72%). Diarrhea is less common. The most common comorbidity is hypertension. Upon admission, the proportion of bilateral pulmonary involvement and interstitial abnormalities evidenced by chest computed tomography (CT) imaging in severe cases was 60.87% and 27.54%, respectively. Compared with patients with nonsevere disease, those with severe disease showed obvious lymphocytopenia. Elevated level of lactate dehydrogenase (LDH), C-reactive protein (CRP), ferritin and D-dimer was found in most cases. Two patients (2.9%) needed transfer to the intensive care unit. Baseline immunological parameters and most of the inflammatory parameters were basically within the normal range. However, baseline interleukin-6 (IL-6) was significantly increased in severe type, which was closely related to the maximal body temperature during hospitalization and to CT findings. Baseline IL-6 was also significantly related to the increase of baseline level of CRP, LDH, ferritin and D-dimer. The increase of baseline IL-6 level suggests that it may positively correlate with the severity of COVID-19. Among the 30 severe type patients whose IL-6 was assessed before and after treatment, significant decrease in IL-6 and improved CT assessment was found in 25 patients after treatment. Whereas the IL-6 level was further increased in 3 cases, which was closely related to disease progression. It is suggested that IL-6 may be used as a marker for disease monitoring in severe COVID-19 patients. ConclusionsOn admission, the baseline level of IL-6, CRP, LDH and ferritin was closely related to the severity of COVID-19, and the elevated IL-6 was significantly related to the clinical manifestation of severe type patients. The decrease of IL-6 was closely related to treatment effectiveness, while the increase of IL-6 indicated disease exacerbation. Collectively, the dynamic change of IL-6 level can be used as a marker for disease monitoring in patients with severe COVID-19.
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Objective@#To investigate the imaging appearance of CT and MRI in retroperitoneal dedifferentiated liposarcoma (DDL) based on pathological findings.@*Methods@#Twelve patients with retroperitoneal DDL (13 lesions) who were surgically and pathologically confirmed were retrospectively collected in the Cancer Hospital of Chinese Academy of Medical Sciences. The correlation of CT and MRI features with histopathologic findings was analyzed.@*Results@#The CT and MRI images of retroperitoneal DDLs were large, heterogeneous soft-tissue masses, mostly lobulated (30.8%, 4/13) or multinodular (46.2%, 6/13), invading adjacent anatomic structures (46.2%, 6/13). The lesions contained different proportions of fatty and non-fatty components, and usually with clear boundaries. The CT images of dedifferentiated components showed non-fatty masses of soft tissue density or mixed density, among which ground-glass nodules may be related to mucinous components. Occasionally calcification or ossification was seen (45.5%, 5/11). The contrast-enhanced CT and MRI images of non-fatty components commonly showed intense heterogeneous enhancement (84.6%, 11/13), central cystic changes and necrosis (61.5%, 8/13), pathologically corresponding to multiple types of soft tissue sarcomas without significant specificity. The well-differentiated components were fatty masses with irregular fibrous septa or soft tissue nodules, which is pathologically corresponding to well differentiated liposarcoma. Lymph node or distant metastasis was rare.@*Conclusions@#The imaging manifestations of retroperitoneal DDLs are diverse and closely related to the proportion and distribution of different components. CT, MRI and contrast-enhanced imaging has a certain diagnostic value for retroperitoneal DDLs.
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To demonstrate the effect of AB serum on terminal erythroid differentiation ex vivo. Methods: After separation of CD34+ cells from cord blood, the cells were cultured and divided into a control group and an experimental group. The effects of AB serum were examined by the expressions of different markers (GPA, Band3 and α4-integrin) for erythroblast differentiation and enucleation by flow cytometry. Results: The CD34+ cells were successfully differentiated to enucleated red blood cells. There were evident differences among the expressions of GPA, Band3 and α4-integrin between the 2 groups. The percentage of GPA positive cells in the experimental group was bigger than that in the control group in every time point. The expression of Band3 in the experimental group was higher than that in the control group. The expression of α4-integrin in the experimental group was lower than that in the control group. In addition, the enucleation rate in the experimental group was higher than that in the control group. Conclusion: AB serum can promote the cell differentiation and enucleation during terminal erythroid differentiation in vitro.
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Humans , ABO Blood-Group System , Blood , Physiology , Anion Exchange Protein 1, Erythrocyte , Metabolism , Antigens, CD34 , Blood , Cell Differentiation , Genetics , Physiology , Cell Nucleus , Cells, Cultured , Erythrocytes , Physiology , Erythropoiesis , Genetics , Physiology , Fetal Blood , Cell Biology , Physiology , Flow Cytometry , Glycophorins , Metabolism , Integrin alpha4beta1 , MetabolismABSTRACT
The vectors play an important role in gene therapy by transporting the therapy genes into target tumor cells. At present there are mainly two kinds of gene vectors, including biological and non-biological vectors. Biological vectors, including virus, bacteria and stem cells, have good target activity and can efficiently delivery the genes into target tumor cells, but have potential genetic hazards.However, non-biological vectors, including liposomes and nanoparticles, can be simplely prepared with high genetic safety, but they can not transport the genes efficiently. Therefore, establishing safe and effective malignant glioma gene treatment system and finding gene vectors with good target activity become the research focus.
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Objective:To explore the role of MVD, bFGF and VEGF in OMFS and the relationship between them. Methods: Immunochemistry streptavidin peroxidase method was used to detect the MVD and the bFGF, VEGF expressions in 57 cases of OMFS samples. Results: MVD, bFGF and VEGF expressions were different between OMFS and benign. The close correlations were found among the expressions of VEGF, bFGF and MVD. Conclusion: The expressions of VEGF and bFGF are associated with MVD which indicated that VEGF and bFGF may play important roles in angiogenesis.
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Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.
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Objective To investigate the situation of pulmonary tuberculosis among the in-school students and to provide the data for improvement of pulmonary tuberculosis control and prevention.Methods All the pulmonary tuberculosis cases of in-school student's registration cards registered during 2008 in Hefei antituberculosis station were analyzed,and the PPD and X ray were used for disease screening.Results There were 168 student patients with pulmonary tuberculosis,physical examination was the main way to find patients.The positive of mycobacterium tuberculosis examination in tuberculosis patients was highest in junior high school.The results of PPD test showed the rate of strongly positive in close contactors was 13.8%,and four new patients were diagnosed.Conclusions Study shows the pulmonary tuberculosis epidemiological and prevention condition among students sub-population are still serious,so stricter control measures should be improved in the future.
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<p><b>OBJECTIVE</b>Research was done on the possible roles of beta-catenin, p53 and proliferating cell nuclear antigen (PCNA) in the carcinogenesis of colorectal adenoma (CRA).</p><p><b>METHODS</b>beta-catenin and p53 and PCNA expressions were studied with immunohistochemical stain in 77 specimens of CRA together with mild epithelial dysplasia (CRA-MD), CRA with moderate/severe epithelial dysplasia (CRA-D/SD) and CRA with cancerous changes (CRA-C).</p><p><b>RESULTS</b>The percentage of abnormal expression of beta-catenin increased during the transition from CRA-MD to CRA-D/SD to CRA-C (P < 0.01). The nuclear expressions of beta-catenin in CRA-D/SD and CRA-C were all significantly higher than that in CRA-MD. Expression of p53 and PCNA were increased from CRA-MD to CRA-D/SD to CRA-C, with the positive rates in these three groups of 10.3%, 43.8%, 75.0% for p53 and 17.2%, 62.5%, 87.5% for PCNA, respectively. 69.7% of cases with positive nuclear beta-catenin expression showed strong PCNA positivity which was much higher than the 36.4% of cases without nuclear beta-catenin expression (P < 0.05). The percentage of strong PCNA expression in the p53 positive cases was significant higher than that in cases with negative p53 expression (72.4% vs 37.5%, P < 0.05). Nuclear beta-catenin and p53 co-expression rates in CRA-C reached 50%.</p><p><b>CONCLUSION</b>beta-catenin, p53 and PCNA may play important roles in the carcinogenesis of colorectal adenoma.</p>
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Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Biomarkers, Tumor , Colorectal Neoplasms , Diagnosis , Metabolism , Cytoskeletal Proteins , Immunohistochemistry , Prognosis , Proliferating Cell Nuclear Antigen , Trans-Activators , Tumor Suppressor Protein p53 , beta CateninABSTRACT
0.05),that of AR 84.21% and 50.00% (P0.05). Conclusions: The pathogenesis of SS salivary glands appears to be linked to decrease or loss of topical action of androgens.Sex hormones act on epithelial cells of salivary glands through corresponding receptors.
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Objective To study the expressions of fragile histidine triad (FHIT) and hMLH_1 in oral squamous cell carcinoma (OSCC) and their correlativity. Methods Immunohistochemistry was used to detect the expressions of FHIT and hMLH_1 in 69 OSCC and 40 normal oral mucosa. The data was analyzed by Chi-square test. Results The positive rate of FHIT and hMLH_1 in OSCC (46.4% and 47.8%) was lower than that in normal oral mucosa (77.5% and 77.5%), with statistically significant difference (P