ABSTRACT
Since cytokine--mobilization for granulocyte collections in random donors--is not licensed in Austria, we investigated in a prospective analysis the effects of prednisolone or dexamethasone (DXM) in 153 donors (35 women/118 men) and in the collected products. Either prednisolone (50 mg) intravenously 4 h before granulocyte collection or DXM (8 mg) orally 12 h before collection was given. Collections were performed with the Cobe Spectra (CardianBCT, Lakewood). We recorded a significantly higher percentage of granulocytes in the peripheral blood of the DXM. However, the collected number of granulocytes was equal in both groups.
Subject(s)
Blood Component Removal/methods , Dexamethasone/administration & dosage , Granulocytes/cytology , Prednisolone/administration & dosage , Administration, Oral , Adult , Austria , Blood Donors , Female , Granulocytes/drug effects , Granulocytes/metabolism , Humans , Injections, Intraventricular , Male , Middle Aged , Prospective Studies , Young AdultABSTRACT
BACKGROUND: Currently only indirect measures are required for monitoring the function of platelets in platelet concentrates (PC). METHODS: This is an overview on currently available commercialized methods that have been used to determine platelet function in donors, concentrates and after transfusion. We show examples for the application of the no/low shear methods light-transmission aggregometry, flow cytometry, multiple electrode aggregometry, thrombelastography and dynamic light scattering, and those applying high shear, the platelet function analyzer-100, and the cone and plate analyzer. Advantages and disadvantages of the various methods to screen donors, evaluate the haemostatic properties maintained in the PC and after transfusion are discussed, based on considerations of platelet physiology, and the feasibility of the various procedures. This survey focuses on reports from the last 10 years, as the technology for the production of PCs has advanced significantly during the last few years. CONCLUSION: Specific aspects of platelet function can be assessed by the no/low shear methods, while the high shear methods provide more general analysis of platelet haemostatic competence. Yet, there is no strong evidence that the in vitro data correspond with the clinical outcome.
