ABSTRACT
Current point cloud extraction methods based on photogrammetry generate large amounts of spurious detections that hamper useful 3D mesh reconstructions or, even worse, the possibility of adequate measurements. Moreover, noise removal methods for point clouds are complex, slow and incapable to cope with semantic noise. In this work, we present body2vec, a model-based body segmentation tool that uses a specifically trained Neural Network architecture. Body2vec is capable to perform human body point cloud reconstruction from videos taken on hand-held devices (smartphones or tablets), achieving high quality anthropometric measurements. The main contribution of the proposed workflow is to perform a background removal step, thus avoiding the spurious points generation that is usual in photogrammetric reconstruction. A group of 60 persons were taped with a smartphone, and the corresponding point clouds were obtained automatically with standard photogrammetric methods. We used as a 3D silver standard the clean meshes obtained at the same time with LiDAR sensors post-processed and noise-filtered by expert anthropological biologists. Finally, we used as gold standard anthropometric measurements of the waist and hip of the same people, taken by expert anthropometrists. Applying our method to the raw videos significantly enhanced the quality of the results of the point cloud as compared with the LiDAR-based mesh, and of the anthropometric measurements as compared with the actual hip and waist perimeter measured by the anthropometrists. In both contexts, the resulting quality of body2vec is equivalent to the LiDAR reconstruction.
ABSTRACT
The Commission for Accreditation and Surveillance of Laboratories Practicing DNA Paternity Tests (created by the Colombian Law 721/2001) set up sub-commission to review the current basic Colombian standards required for paternity testing laboratories and make specific recommendations re the pertinent technical aspects in Colombia, taking ISO 17025 as current reference. This document contains such recommendations for Colombia.
Subject(s)
DNA Fingerprinting/standards , Laboratories/standards , Paternity , Colombia , HumansABSTRACT
La "Comisión de Acreditación y Vigilancia de los Laboratorios que practican las pruebas de paternidad o maternidad con marcadores genéticos de ADN", creada por la Ley 721 de 2001, constituyó una Sub-comisión para revisar las normas colombianas sobre los Estándares Básicos Requeridos en los Laboratorios de Pruebas de Paternidad, para que específicamente recomendara los aspectos técnicos pertinentes en Colombia, tomando como referente permanente la Norma ISO 17025. En este documento se consignan estas recomendaciones para Colombia.
Subject(s)
Humans , DNA Fingerprinting/standards , Laboratories/standards , Paternity , ColombiaABSTRACT
We report here a review of the seventh mitochondrial DNA (mtDNA) exercise undertaken by the Spanish and Portuguese working group (GEP) of the International Society for Forensic Genetics (ISFG) corresponding to the period 2003-2004. Five reference bloodstains from five donors (M1-M5), a mixed stain of saliva and semen (M6), and a hair sample (M7) were submitted to each participating laboratory for nuclear DNA (nDNA; autosomal STR and Y-STR) and mtDNA analysis. Laboratories were asked to investigate the contributors of samples M6 and M7 among the reference donors (M1-M5). A total of 34 laboratories reported total or partial mtDNA sequence data from both, the reference bloodstains (M1-M5) and the hair sample (M7) concluding a match between mtDNA profiles of M5 and M7. Autosomal STR and Y-STR profiling was the preferred strategy to investigate the contributors of the semen/saliva mixture (M6). Nuclear DNA profiles were consistent with a mixture of saliva from the donor (female) of M4 and semen from donor M5, being the semen (XY) profile the dominant component of the mixture. Strikingly, and in contradiction to the nuclear DNA analysis, mtDNA sequencing results yield a more simple result: only the saliva contribution (M4) was detected, either after preferential lysis or after complete DNA digestion. Some labs provided with several explanations for this finding and carried out additional experiments to explain this apparent contradictory result. The results pointed to the existence of different relative amounts of nuclear and mtDNAs in saliva and semen. We conclude that this circumstance could strongly influence the interpretation of the mtDNA evidence in unbalanced mixtures and in consequence lead to false exclusions. During the GEP-ISFG annual conference a validation study was planned to progress in the interpretation of mtDNA from different mixtures.
