Fast FRAP-SIA method to determine antioxidant capacity.

One of the most used methods to measure antioxidant capacity in food is the ferric reducing antioxidant power (FRAP) test, which is simple, sensitive, and economical, nevertheless has long analysis times, causing measurement errors due to the instability of the FRAP reagent due to its precipitation sequential injection analysis (SIA) is a flow technique that can correct these disadvantages because it is more quickly. So, a novel FRAP-SIA method was developed to evaluate the antioxidant capacity. The system was optimized using a central composite design for hydrodynamic and chemical factors, resulting in a flow rate of 35 µL s-1, and aspirate volumes of 33 µL-38 µL-33 µL for the sequence (FRAP-Antioxidant-FRAP). FRAP reagent was prepared with an HCl solution at 0.005 mol L-1, improving its stability 24 times, concerning when it is in acetate buffer at pH 3.6. The method showed excellent accuracy (RSD <3%) with a LOD of 1.0 µmol L-1 of Trolox for a linear range of 5-120 µmol L-1. The reaction time was diminished by 96% concerning the FRAP-microplate assay (from 30 min to 1.2 min). The method was applied in beverages and extracts, obtaining recovery values ranging from 91.24 to 114.22%.

Fast ORAC-SIA method for antioxidant capacity determination in food samples.

A novel fluorescent ORAC-SIA method to determine antioxidant capacity in several food samples using fluorescein as the probe was developed. The optimization of the method was through a multivariable design, decreasing the analysis time to 5 min and the AAPH concentration to 67% compared with 90 min in the standard 96-well microplate method. The aspiration order was AAPH-sample/standard-fluorescein injected into a stream of a water-based carrier. The calculation of the antioxidant capacity was done from the fluoresceine peak heigh, so neither delay time nor area measurement was necessary. The proposed method showed excellent precision (RDS < 3%) with a LOD of 3.13 µmol L-1 and recoveries from 90% to 107%. The results from the ORAC-SIA method did not show a significant difference from the microplate method.