Influence of Short and Medium Distance Road Transport on the Acute Phase Proteins in Horses.

Horse transport is a common practice and is usually associated as a cause of stress in animals, with consequences for their well-being. There are several of evidence that stress can increase an acute phase response. The aim of this study was to verify whether the road transport of horses over distances of 50 and 300 kilometers induces changes in the values of acute phase proteins. To do this, the serum SDS-PAGE was performed and the bands obtained were identified by mass spectrometry (MALDI-TOF). The blood samples were collected in tubes without anticoagulant to obtain the serum, and the evaluations occurred before the road transportation (T0), immediately after the journey (T1), six hours later (T2), and 24 hours (T3), 48 hours (T4), 72 hours (T5), 96 hours (T6), 120 hours (T7) and 144 hours (T8) after the end of the trip. All analyzes were performed using the Minitab 17 statistical package, and significance was considered when P<0.05. The APPs found through SDS-PAGE and properly identified were α2-macroglobulin, ceruloplasmin, transferrin, albumin, α1-antitrypsin, haptoglobin, apolipoprotein alpha 1, and α1-acid glycoprotein. No differences were observed in the concentration values between 50 and 300 km or between the moments after each route. The distances covered with the horses were not challenging enough to provoke an acute phase response reflected in changes in APPs.

Profile of Acute-Phase Proteins of Horses Submitted to Low-Level Show Jumping Classes.

The objective of this study was to evaluate the serum proteinogram, identifying and quantifying the acute-phase proteins (APPs) of horses used in show jumping activity with obstacles of a meter in height. As it is an equestrian sport that involves high intensity and excessive impact, the possibility of injury is relevant. The serum of 10 horses was evaluated in a competition for beginners. The material was collected at rest (T0), immediately after exercise (T1), 30 minutes after the effort (T2), 1 hour after the effort (T3), and 24 hours after the effort. Acute-phase proteins were separated by polyacrylamide gel electrophoresis and their concentrations determined by computerized densitometry. Protein identification was performed using mass spectrometry. The data were evaluated using analysis of variance for repeated measures, considering the level of significance of P < .05. Eight APPs were identified: α2-macroglobulin (α2-macro), ceruloplasmin (Cp), transferrin (Trf), albumin (Alb), α1-antitrypsin (α1-atp), haptoglobin (Hp), acid glycoprotein (AGP), and apolipoprotein A1 (Apo A1). There was a difference in Cp, AGP, and Apo A1 between moments. As the other proteins were not influenced by exercise, they were established as a valuable resource in the monitoring of inflammatory processes and an important complementary element in controlling the impact of training on the animals, thus guaranteeing their welfare.