ABSTRACT
OBJECTIVE: The objective of this study was to use high-throughput sequencing to describe the vaginal eukaryotic DNA virome in patients undergoing in vitro fertilisation (IVF) to examine associations between the vaginal virome, antibiotic exposure and IVF outcomes. DESIGN: Prospective exploratory study. SETTING: Single academic fertility centre. POPULATION: Subfertile women age 18-43 years undergoing their first IVF cycle with a fresh embryo transfer. METHODS: The primary exposure was prophylactic azithromycin or no azithromycin before IVF. A mid-vaginal swab was obtained at the time of embryo transfer for virome analysis. MAIN OUTCOME MEASURES: The primary outcomes compared between exposure groups were characteristics of vaginal virome and clinical pregnancy rates. Secondary outcomes were virome associations with number of oocytes retrieved, number of blastocysts and implantation rate. RESULTS: Twenty-six women contributed a vaginal swab before embryo transfer. There were no significant differences in IVF outcomes between azithromycin groups. There was no association between viral diversity and clinical pregnancy overall. A higher diversity of herpesviruses and α-papillomaviruses was observed in samples from the azithromycin-treated group compared with the no azithromycin group (P = 0.04). In women that received azithromycin, viral diversity was higher in the group that did not achieve clinical pregnancy compared with those who did (P = 0.06). CONCLUSIONS: We demonstrate that the vaginal eukaryotic virome in women undergoing IVF is associated with antibiotic exposure. Additionally, we demonstrate an inverse trend between viral diversity and pregnancy, with a higher number of viruses detected associated with failure to achieve clinical pregnancy in the azithromycin group. TWEETABLE ABSTRACT: Higher viral diversity is associated with prophylactic antibiotic exposure in subfertile women undergoing IVF.
Subject(s)
Eukaryota/physiology , Fertilization in Vitro , Infertility/therapy , Microbiota , Vagina/virology , Adult , Anti-Bacterial Agents/therapeutic use , DNA, Viral/physiology , Embryo Transfer , Female , Herpesviridae , Humans , Microbiota/genetics , Microbiota/immunology , Papillomaviridae , Pregnancy , Prospective Studies , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 16S/immunology , Sequence Analysis, DNA , Vagina/microbiologyABSTRACT
A study evaluating use, compliance and activities of a child health care program at a health center in Granada. The data, taken over a period of five years, showed that 75.2% of one month old infants and 94% of three month old infants used the program. The three month old group showed the highest rate of compliance, where as the group of two year old infants had the lowest. Clinical visits demonstrated a high level of use, between 84-100%. Data about lactating mothers and vaccinations showed a low number of patients recorded, which is similar to the data published in other journals.
