[A susceptibility study of Mycobacterium avium-intracellulare strains using the broth macrodilution method]. / Estudio de la susceptibilidad en cepas de Mycobacterium avium-intracellulare empleando el método de macrodilución en caldo.

The macrobroth dilution method was used for determining the resistance and/or sensitivity of 10 nontuberculous mycobacteria strains from Mycobacterium avium-intracellulare (MAI) complex isolated from patients presenting with respiratory symptoms. Drugs employed were as follows: isoniazid, ethambutol, rifampicin, streptomycin, gentamicin and amikacin. With respect to susceptibility of the strains studied, the greater sensitivity was found to be against ethambutol, amikacin, and gentamicin. The possibility of replacing the Middlebrook 7H9 liquid culture medium by the UIT-L liquid culture medium was analyzed. Normalization of the method was attained and a shortening of the reading time when using the UIT-L culture medium was also obtained.

[Sensitivity of Mycobacterium fortuitum by the broth microdilution method determining the inhibitory minimal concentration]. / Estudio de la sensibilidad de Mycobacterium fortuitum por el método de la microdilución en caldo determinando la concentración mínima inhibitoria.

A study was conducted on 40 Mycobacterium fortuitum strains isolated from symptomatic patients suffering from respiratory diseases and skin lesions. Their susceptibility to different antimicrobial agents was determined by the broth microdilution method, measuring minimal inhibitory concentration. There was sensitivity of the strains to gentamicin and tetracycline as well as resistance to the tuberculostatic drugs used (isoniazid and ethambutol) and cefalotin.

[Phage typing of Mycobacterium tuberculosis using the overlay technique. Preliminary study]. / Fagotipaje de Mycobacterium tuberculosis por la técnica de la doble capa (overlay). Estudio preliminar.

One hundred and fifty strains of Mycobacterium tuberculosis were classified by the phagotyping technique, using the double layer method described by Adams and modified by Jones. The efficacy of this method was studies in comparison with the method claimed by Redmond and Ward and traditionally employed for Mycobacteria typing. Preliminary Results obtained have allowed to prove its advantages. A 96% of coincidence with respect to the traditional method was reported.

[Non-tuberculous mycobacteria isolated in Cuba during the 1985-1989 period. Preliminary report]. / Estudio de micobacterias no tuberculosas aisladas en Cuba durante el período 1985-1989. Reporte preliminar.

A number of 1,061 strains of nontuberculous mycobacteria referred to "Pedro Kouri" Institute of Tropical Medicine during the period of 1985-1989 were studied. Strains were from Provincial Centers of Hygiene and Epidemiology of the country. According to the results obtained, most of the strains classified are found in groups III and IV according to the criteria of Runyon (54, 76, and 36%, respectively). Species with a greater frequency belong to the complex MAI and M. fortuitum.

[Phage typing of Mycobacterium tuberculosis strains coming from Nicaragua (preliminary report)]. / Fagotipaje de cepas de Mycobacterium tuberculosis procedentes de Nicaragua (reporte preliminar).

Fifty Nicaraguan strains of Mycobacterium tuberculosis were classified with a scheme of 9 specific phages. A preliminary distribution of characteristic phagotypes of the area is reported, with prevalence of phagotype I (intermedial) (66%), presence of phagotype A (34%) and absence of the rest of the types. Inclusions of phagotypes Bo4 and F-o-WJ within the classification scheme, as well as practical usefulness of the technique in studies directed to disease control, are valued.

[Study using phagetype markers of Mycobacterium tuberculosis strains from Ethiopia. A preliminary study]. / Estudio mediante marcadores fagotipicos de cepas de Mycobacterium tuberculosis procedentes de Etiopia. Estudio preliminar.

Twenty strains of Mycobacterium tuberculosis from Addis Ababa (Ethiopia) are studied by means of the phage typing technique in mycobacteria. The results obtained show the preliminary distribution of phage types belonging to type I (intermediate) and absence of the remaining types. The lysis pattern of autochthonous M. tuberculosis is characterized by a prevalence of sensitivity to phages DS6A, GS4E, BG1, and D34. The early results of sensitivity obtained with phage Bo4 and its assessment within the current scheme for M. tuberculosis classification.

Micobacteriofagos III. (Articulo de revision). / Mycobacteriophages III.(Review article)

Se continua con el estudio y analisis de tecnicas de fagotipaje en micobacterias (micobacteriofagos), a traves de la revision de una serie de articulos publicados al respecto