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1.
BMC Genomics ; 14: 160, 2013 Mar 09.
Article in English | MEDLINE | ID: mdl-23497397

ABSTRACT

BACKGROUND: The brown planthopper (Nilaparvata lugens) is one of the most serious rice plant pests in Asia. N. lugens causes extensive rice damage by sucking rice phloem sap, which results in stunted plant growth and the transmission of plant viruses. Despite the importance of this insect pest, little is known about the immunological mechanisms occurring in this hemimetabolous insect species. RESULTS: In this study, we performed a genome- and transcriptome-wide analysis aiming at the immune-related genes. The transcriptome datasets include the N. lugens intestine, the developmental stage, wing formation, and sex-specific expression information that provided useful gene expression sequence data for the genome-wide analysis. As a result, we identified a large number of genes encoding N. lugens pattern recognition proteins, modulation proteins in the prophenoloxidase (proPO) activating cascade, immune effectors, and the signal transduction molecules involved in the immune pathways, including the Toll, Immune deficiency (Imd) and Janus kinase signal transducers and activators of transcription (JAK-STAT) pathways. The genome scale analysis revealed detailed information of the gene structure, distribution and transcription orientations in scaffolds. A comparison of the genome-available hemimetabolous and metabolous insect species indicate the differences in the immune-related gene constitution. We investigated the gene expression profiles with regards to how they responded to bacterial infections and tissue, as well as development and sex expression specificity. CONCLUSIONS: The genome- and transcriptome-wide analysis of immune-related genes including pattern recognition and modulation molecules, immune effectors, and the signal transduction molecules involved in the immune pathways is an important step in determining the overall architecture and functional network of the immune components in N. lugens. Our findings provide the comprehensive gene sequence resource and expression profiles of the immune-related genes of N. lugens, which could facilitate the understanding of the innate immune mechanisms in the hemimetabolous insect species. These data give insight into clarifying the potential functional roles of the immune-related genes involved in the biological processes of development, reproduction, and virus transmission in N. lugens.


Subject(s)
Genome, Insect , Hemiptera/genetics , Immunity, Innate/genetics , Transcriptome , Amino Acid Sequence , Animals , Carrier Proteins/chemistry , Carrier Proteins/classification , Carrier Proteins/genetics , Hemiptera/growth & development , Hemiptera/metabolism , Insect Proteins/chemistry , Insect Proteins/classification , Insect Proteins/genetics , Molecular Sequence Data , Phylogeny , Sequence Alignment , Signal Transduction , Toll-Like Receptors/genetics , Toll-Like Receptors/metabolism
2.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-297046

ABSTRACT

<p><b>OBJECTIVE</b>To scientifically assess effectiveness and safety of mono-use fu-needle.</p><p><b>METHODS</b>Sixty cases of periarthritis of shoulder were randomly divided into a fu-needle group and a routine acupuncture group. The fu-needle group were treated with fu-needle, and the routine acupuncture group were treated with a needle of 0.38 mm in diameter and 40 mm in length. The articular mobility of the most limited direction, soft tissue pain self-rating score and self-rating score of shoulder tenderness before and after treatment, stabbing times and degree at inserting the needle, scattering and during retention of the needle, and bleeding at withdrawing the needle were compared in the two groups.</p><p><b>RESULTS</b>The fu-needle group was better than the routine acupuncture group in improvement of mobility-related pain, tenderness, and the articular mobility of the most limited direction, indicating that the transient effect in the fu-needle group was better than the routine acupuncture group, and the stabbing times and degree at insertion of the needle were less than the routine acupuncture group. And there was no significant difference between the two groups in stabbing times and degree at scattering and retaining the needle and bleeding times in withdrawing the needle.</p><p><b>CONCLUSION</b>The superficial needling therapy with mono-use fu-needle is more effective and more safe than the routine acupuncture for treatment of periarthritis of shoulder.</p>


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Acupuncture Therapy , Methods , Periarthritis , Therapeutics , Range of Motion, Articular , Shoulder Joint , Shoulder Pain , Therapeutics
3.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-267315

ABSTRACT

<p><b>OBJECTIVE</b>To observe changes of plasma peptide YY (PYY) in acupuncture for slimming.</p><p><b>METHODS</b>Thirty-four cases of simple obesity were treated with acupuncture for 3 courses. Body mass index (BMI), and fat percent (F%), and insulin, PYY, blood fat, and blood sugar levels before and after treatment were determined, with 20 normal persons used as controls.</p><p><b>RESULTS</b>The clinical total effective rate was 88.23%. Acupuncture could increase the decreased insulin sensitive index and PYY level (both P < 0.01), with no correlativity between them (P > 0.05).</p><p><b>CONCLUSION</b>Regulation of PYY is possibly one of the mechanisms of acupuncture in slimming, but the relation of PPY with insulin resistance remains to be studied.</p>


Subject(s)
Humans , Body Mass Index , Insulin , Blood , Insulin Resistance , Obesity , Therapeutics , Peptide YY
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