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1.
Guang Pu Xue Yu Guang Pu Fen Xi ; 27(11): 2313-6, 2007 Nov.
Article in Chinese | MEDLINE | ID: mdl-18260420

ABSTRACT

According to the principle that Zn(OH)+ produced by ZrOCl2 upon hydrolysis in water can react to form a fluorescent derivative with fructose, a method for the quantitative determination of fructose has been proposed. The fluorescence of the derivative enhanced at 408 nm in NH4Cl medium has a direct ratio with fructose in the linear range of 0.5-25 microg x mL(-1). When reaction conditions such as a temperature of 70 degrees C and a time of 15 min are used, the detection limits is 0.071 mg x mL(-1). The proposed method was also applied successfully to the determination of fruit, satisfaction with high accuracy and good reproduction.


Subject(s)
Fructose/analysis , Glucose/analysis , Spectrometry, Fluorescence/methods , Ammonium Chloride/chemistry , Fruit/chemistry
2.
Guang Pu Xue Yu Guang Pu Fen Xi ; 23(3): 597-9, 2003 Jun.
Article in Chinese | MEDLINE | ID: mdl-12953552

ABSTRACT

The optimum conditions of the colour reaction of Cobalt, Nickel and Zinc with 5-Br-PADAP were established in the presence of the sensitizer/solubilizer CPB. The absorbances of the reaction system Co-Ni-Zn- (5-Br-PADAP) -CPB were measured in the spectral range of 530-590 nm at every 2 nm intervals. A chemometric method of data treatment was studied. Through the comparative study of data treatment by PLS method and by PLS after wavelet transformation, it was found that the recovery of determination is significantly improved by using the latter method.


Subject(s)
Cobalt/analysis , Nickel/analysis , Signal Processing, Computer-Assisted , Zinc/analysis , Algorithms , Fourier Analysis , Least-Squares Analysis , Spectrophotometry/methods
3.
Microbiology ; (12)1992.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-686213

ABSTRACT

To investigate the bioactivity of Nocardia rubra Cell (NC), the mice were used to assay the toxicity, the effects on immune organs, phagocytes of peritoneal macrophage and the antitumor activity by perfusion of NC to the stomach of mice. Results indicated that NC could obviously stimulate in vitro the phagocytosis of peritoneal macrophage from mice, and remarkably inhibit the growth of S180 in mice, and its LD50 was more than 10 g/kg. In conclusion, NC had low toxicity, it could significantly enhance the organism immunologic function and had obvious antitumor effect and the anti-infection effect against a pathogenic microorganism.

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