ABSTRACT
OBJECTIVE: This study aimed to analyze the clinical efficacy of the Jianpi Shengxue tablet for treating renal anemia. METHODS: A total of 200 patients with renal anemia from December 2020 to December 2022 were enrolled and randomly divided into two groups. Patients in the control group were treated with polysaccharide-iron complex, and those in the experimental group were administered Jianpi Shengxue tablet. After 8 weeks of continuous treatment, the therapeutic outcomes regarding anemia were compared between the two groups. RESULTS: After treatment, the red blood cell (RBC) count, hematocrit (HCT), reticulocyte percentage (RET), ferritin (SF), serum iron (SI), transferrin saturation (TSAT), and serum albumin (ALB) all increased (P<0.01), and the clinical symptom score and total iron binding capacity decreased (P<0.01) in the experimental group. Moreover, the improvements in RBC, HCT, RET, SF, SI, TAST, ALB, and clinical symptoms (fatigue, anorexia, dull skin complexion, numbness of hands and feet) in the experimental group were significantly greater than those in the control group (P<0.05). The total effective rate for treating renal anemia was significantly higher in the experimental group than in the control group (P<0.01). CONCLUSION: The Jianpi Shengxue tablet demonstrates efficacy in treating renal anemia, leading to significant improvements in the laboratory examination results and clinical symptoms of patients with renal anemia.
Subject(s)
Drugs, Chinese Herbal , Iron , Nutritional Status , Humans , Male , Female , Iron/metabolism , Iron/blood , Middle Aged , Drugs, Chinese Herbal/administration & dosage , Drugs, Chinese Herbal/pharmacology , Drugs, Chinese Herbal/therapeutic use , Prospective Studies , Nutritional Status/drug effects , Tablets , Adult , Anemia/drug therapy , Anemia/metabolism , Anemia/blood , Aged , Treatment Outcome , Hematocrit , Ferritins/blood , Erythrocyte CountABSTRACT
OBJECTIVE: To observe the effect of electroacupuncture(EA)on renal function and autophagy-related protein expression in diabetic nephropathy (DN) ratsï¼so as to explore its mechanisms underlying improvement of DN. METHODS: Male Wistar rats were randomly divided into normal controlï¼DN model and EA groups (n=8 rats in each group). The DN model was established by high-fat diet (for 4 weeks) + streptozotocin (STZï¼35 mg/kgï¼i.p.). EA (2 Hz/15 Hzï¼1 mA) was applied to bilateral "Shenshu"(BL23)and "Zusanli"(ST36)for 20 minï¼5 times a week for 6 consecutive weeks. The urine volume in 24 h (24 h-UV) was recordedï¼the levels of urine protein in 24 h (24 h-UP)ï¼blood urea nitrogen (BUN) and serum creatinine (Scr) were detected by using an automatic biochemical analyzerï¼and the fasting blood glucose (FBG) was detected using rapid blood glucose paper test. The histopathological changes of glomerulus of the left kidney were observed after periodic acid-Schiff stain (PAS) and the ultra-microstructural changesï¼the numbers of autophagosomes and autophagic vacuoles of the right kidney were observed by transmission electronic microscope (TEM). The expression and localization of podocyte marker protein Nephrin was detected by using immunofluorescence assayï¼and the expression levels of autophagy-related proteins microtubule associated protein 1 (LC3)ï¼Beclin-1ï¼p62 and Nephrin in the kidney tissue measured by Western blot. RESULTS: Before treatment,compared with the normal control groupï¼the levels of 24 h-UVï¼24 h-UP and FBG were significantly increased (P<0.01) in the model and EA groups. After treatment, compared with the normal groupï¼the levels of 24 h-UVï¼24 h-UPï¼FBGï¼BUNï¼Scr and p62 protein expression were signi-ficantly increased (P<0.01)ï¼and the expression levels of LC3â ¡ï¼Beclin-1 and Nephrin proteins and ratio of LC3â ¡/â were significantly decreased (P<0.01) in the model group. Compared with the model groupï¼the levels of 24 h-UVï¼24 h-UPï¼FBGï¼BUN and p62 were apparently decreased (P<0.01ï¼P<0.05)ï¼while the expression levels of LC3â ¡ï¼Beclin-1 and Nephrin proteins and ratio of LC3â ¡/â were obviously up-regulated (P<0.01ï¼P<0.05) in the EA group. No significant changes were found in the level of Scr after EA (P>0.05) and in the expression levels of LC3 â after modeling and EA (P>0.05). Outcomes of PAS showed blurred structure of the glomerulus with obvious proliferation of glomerular mesangial cellsï¼increase of cell matrixï¼and thickening of basement membrane of the glomerulus and renal tubuleï¼atrophy of epithelia cells of renal tubules and infiltration of inflammatory cells in the renal interstitum; and TEM revealed fusion and exfoliation of some foot processesï¼bareness or thickening of some basement membraneï¼and reduction of number of autophagosomes or autophagobubbles in podocytes in the model groupï¼which was relatively milder in the EA group. CONCLUSION: EA can effectively alleviate kidney damage in DN ratsï¼which may be related to its function in facilitating autophagy in the kidney.